Bone Marrow Mesenchymal Stem Cells Stabilize Already-formed Aortic Aneurysms More Efficiently than Vascular Smooth Muscle Cells in a Rat Model

Schneider, Fabrice; Saucy, François; Blic, Romain de; Dai, Jianping; Mohand, F.; Rouard, Hélène; Ricco, Jean‐Baptiste; Becquemin, J P; Gervais, Marianne; Allaire, Éric

doi:10.1016/j.ejvs.2013.03.007

Cited by 43 publications

(37 citation statements)

References 24 publications

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“…It has been demonstrated that bone marrow mesenchymal stem cell and SMC transplantation can stabilize growing abdominal aortic aneurysms in rats 8,9 and promote healing of saccular venous pouch aneurysms in dogs. 19 In a rabbit model of elastaseinduced saccular aneurysms, endovascular seeding of bone marrow mesenchymal stem cells promoted thrombus organization and caused the aneurysm to shrink in size.…”

Section: Local Cell Therapy Heals Aneurysms With Degenerated/decellulmentioning

confidence: 99%

“…[5][6][7] Previously it has been shown in models of fusiform aortic aneurysms that restoration of the cell content of the aneurysm with local cell transplantation can reduce the progression of the aneurysm. 8,9 We investigated the hypothesis that loss of mural cells will promote degenerative remodeling of the saccular aneurysm wall in thrombosed aneurysms, and that this degenerative remodeling can be reduced or compensated by direct transplantation of smooth muscle cells (SMCs) into the luminal thrombus.…”

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confidence: 99%

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Intraluminal Cell Transplantation Prevents Growth and Rupture in a Model of Rupture-Prone Saccular Aneurysms

Marbacher

Frösén

Marjamaa

et al. 2014

Stroke

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Background and Purpose-Aneurysm occlusion by intraluminal thrombus formation is the desired effect of all endovascular treatments. Intraluminal thrombus may, however, recanalize and be absorbed, unless it is infiltrated by cells that turn it into fibrous tissue (neointima). Because ruptured aneurysm walls are characterized by loss of smooth muscle cells, we assessed the impact of mural cell loss on wall remodeling of thrombosed aneurysms and investigated whether neointima formation could be enhanced by direct transplantation of cells into the thrombus. Methods-Sidewall aneurysms were microsurgically created in rats (n=81). Certain aneurysms were decellularized.Thrombosis was induced using direct injection of a fibrin polymer into the aneurysm. CM-Dil-labeled smooth muscle cells were injected into 25 of 46 fibrin embolized aneurysms. Recanalization and aneurysm growth were monitored with magnetic resonance angiography. Endoscopy, optical projection tomography, histology, and immunohistochemistry were used to study the fate of transplanted cells, thrombus organization, and neointima formation.

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Section: Local Cell Therapy Heals Aneurysms With Degenerated/decellulmentioning

confidence: 99%

mentioning

confidence: 99%

Intraluminal Cell Transplantation Prevents Growth and Rupture in a Model of Rupture-Prone Saccular Aneurysms

Marbacher

Frösén

Marjamaa

et al. 2014

Stroke

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“…We have previously shown that increasing endothelial progenitors 36 and mesenchymal stem cells 37 by local cell therapy is able to slow the progression of experimental AAAs. In our study, we did not address whether CXCR4 + cell levels were increased into the peripheral blood after chronic AMD treatment.…”

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confidence: 99%

“…34 Over the past decade, our laboratory has promoted cell [35][36][37] or gene 38 therapy to stop further AAA expansion, but these 2 approaches are hampered by technical difficulties and potential high cost for a frequent disease. To this regard, 1 major result of our study is that the blockade of CXCR4 by AMD3100 limits AAA formation in mice but also stabilizes already-formed AAAs in 2 experimental models.…”

mentioning

confidence: 99%

Chemokine (C-X-C Motif) Receptor 4 Blockade by AMD3100 Inhibits Experimental Abdominal Aortic Aneurysm Expansion Through Anti-Inflammatory Effects

Michineau

Franck

Wagner-Ballon

et al. 2014

ATVB

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Objective-Inflammation plays a critical role in the development of abdominal aortic aneurysms (AAAs). Because stromal cell-derived factor 1 (SDF-1) is known for its ability to attract inflammatory cells, we investigated whether SDF-1/ chemokine (C-X-C motif) receptor 4 (CXCR4) axis is expressed in aneurysmal aortic wall and plays a role in AAA physiopathology and asked whether its blockade modulates AAA formation and expansion. Approach and Results-Quantitative real-time polymerase chain reaction analysis showed that SDF-1α and CXCR4 mRNA levels are increased in both human and CaCl 2 -induced mouse AAA wall and are positively correlated to the aortic diameter in mice. ELISA quantification and immunostaining demonstrated that, in mice, aortic SDF-1α is rapidly induced during AAA formation, first by apoptotic vascular smooth muscle cells in the injured media and then by adventitial macrophages once AAA is fully established. Using green fluorescent protein-positive (GFP +/− ) bone marrow transplantation experiments, we demonstrated that aortic SDF-1 overexpression is implicated in the recruitment of bone marrow-derived macrophages within the AAA wall. Furthermore, in mice, blockade of CXCR4 by AMD3100 decreases the infiltration of adventitial macrophages, inhibits AAA formation, and prevents aortic wall destruction. AMD3100 reduces the mRNA levels of MMP-12 and MMP-14 as well as that of inflammatory effectors MCP-1, MIP-1β, MIP-2α, RANTES, IL-1β, IL-6, TNF-α, and E-selectin. Finally, AMD3100 stabilizes the diameter of formed, expanding AAAs in 2 experimental models.

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“…As an illustrative example, the xenograft rat model [6] has been investigated to study aneurysm evolution and expansion in the abdominal aorta. Thus, it has been proved that the injection of mesenchymal stem cells (MSCs) cultured in hydrogel based on hyaluronic acid (HA) can regenerate injured vascular tissue and particularly stabilize the AAA improving thereby the mechanical resistance of the weakened arterial wall [7][8][9]. In this context, authors [10] focused especially on the optimization of the hydrogel based on hyaluronic acid (HA) from a biological point of view through the investigation of the MSC differentiation potential, their phenotype and viability when encapsulated in a hydrogel.…”

Section: Introductionmentioning

confidence: 99%

Influence of viscoelastic properties of an hyaluronic acid-based hydrogel on viability of mesenchymal stem cells

Eddhahak

Zidi

2015

BME

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is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. Abstract. BACKGROUND:The present research is involved in the framework of the biotherapy using mesenchymal stem cells (MSCs). Here, MSC encapsulation in a hydrogel based on hyaluronic acid (HA) is investigated to optimize the composition of the biomaterial. METHODS: Several formulations candidates of the hydrogel (9 in total) are postulated as a scaffold for the 3D MSC culture in order to investigate their potential to mimic the in vivo cellular environment. Rheological measurements in oscillation mode of complex modulus and complex viscosity are performed on the different hydrogels. Biological tests are carried out for the measurement of the cell viability of MSC encapsulated in the hydrogels. RESULTS: Rheological and biological findings are correlated together in order to establish relationships between the viscoelastic properties of the hydrogel and the cellular viability of MSC. CONCLUSIONS:In the light of the viaility results, the composition of the hydrogel was related to the MSC proliferation. Thus, such relations are useful tools for scientists offering them more flexibility in the design of their hydrogels while ensuring an acceptable level of MSC viability.

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Bone Marrow Mesenchymal Stem Cells Stabilize Already-formed Aortic Aneurysms More Efficiently than Vascular Smooth Muscle Cells in a Rat Model

Cited by 43 publications

References 24 publications

Intraluminal Cell Transplantation Prevents Growth and Rupture in a Model of Rupture-Prone Saccular Aneurysms

Intraluminal Cell Transplantation Prevents Growth and Rupture in a Model of Rupture-Prone Saccular Aneurysms

Chemokine (C-X-C Motif) Receptor 4 Blockade by AMD3100 Inhibits Experimental Abdominal Aortic Aneurysm Expansion Through Anti-Inflammatory Effects

Influence of viscoelastic properties of an hyaluronic acid-based hydrogel on viability of mesenchymal stem cells

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