Bone Marrow Mesenchymal Stem Cells

Shi, Songtao; Gronthos, Stan

doi:10.1002/9781118498026.ch11

Cited by 3 publications

(3 citation statements)

References 102 publications

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“…The SEM images of PLA/BFO (line) have more loaded cells, and cell morphologies with cell‐cell contacts are seen exactly (Figure 6C). It is known that the changes in cell morphology indicate cell activity, and this promoted the cellular migration behavior of the cells 58 . As a result, it can be concluded from the images that the observed strong adhesion and proliferation promoted the biocompatibility of all scaffolds.…”

Section: Resultsmentioning

confidence: 86%

Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D‐printed scaffolds

Bedir

Ulag

Aydogan

et al. 2020

Polymers for Advanced Techs

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Electrical stimulation has shown great potential for nerve regeneration processes. This makes it attractive to use electrically active materials in the neural scaffold. In this paper, bismuth ferrite (BFO) nanoparticles were synthesized via co‐precipitation method and incorporated to 10 wt% polylactic acid (PLA) in chloroform to obtain 3D‐printed PLA/BFO biocomposites. The crystallinity of BFO nanoparticles was confirmed by XRD, and we studied its chemical structure with FTIR, as well as the mechanical properties of the 3D‐printed composites. in vitro studies show that 3D‐printed scaffolds have no cytotoxicity and support the proliferation of human adipose‐derived mesenchymal stem cells (hADMSCs). Furthermore, 3D scaffolds embedded with BFO shows the highest cell viability relative to pristine PLA and BFO‐lined PLA scaffolds. A 48 hours electrical stimulation on the hADMSC cultured inside the 3D‐printed BFO‐lined PLA scaffolds indicates that stimulated cells are aligned toward the BFO line. These results could indicate the potential of BFO for directing cells toward damaged tissues.

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Section: Resultsmentioning

confidence: 86%

Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D‐printed scaffolds

Bedir

Ulag

Aydogan

et al. 2020

Polymers for Advanced Techs

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“…Cells with multipotency can also be transformed into many cell types, while those with oligopotency can be transformed into a few cell types and those with unipotency into one cell type (1,2). Mesenchymal stem cells (MSCs) are derived from several organs including the lungs, heart, adipose tissue, bone marrow, and liver (3)(4)(5)(6)(7)(8). MSCs are capable of generating multiple mesenchymal cell lineages under specific culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Investigation of gene expressions in differentiated cell derived bone marrow stem cells during bone morphogenetic protein-4 treatments with Fourier transform infrared spectroscopy

Zafari

Jouni

Ahmadvand

et al. 2017

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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A model was set up to predict the differentiation patterns based on the data extracted from FTIR spectroscopy. For this reason, bone marrow stem cells (BMSCs) were differentiated to primordial germ cells (PGCs). Changes in cellular macromolecules in the time of 0, 24, 48, 72, and 96h of differentiation, as different steps of the differentiation procedure were investigated by using FTIR spectroscopy. Also, the expression of pluripotency (Oct-4, Nanog and c-Myc) and specific genes (Mvh, Stella and Fragilis) were investigated by real-time PCR. However, the expression of genes in five steps of differentiation was predicted by FTIR spectroscopy. FTIR spectra showed changes in the template of band intensities at different differentiation steps. There are increasing changes in the stepwise differentiation procedure for the ratio area of CH2, which is symmetric to CH2 asymmetric stretching. An ensemble of expert methods, including regression tree (RT), boosting algorithm (BA), and generalized regression neural network (GRNN), was the best method to predict the gene expression by FTIR spectroscopy. In conclusion, the model was able to distinguish the pattern of different steps from cell differentiation by using some useful features extracted from FTIR spectra.

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“…STRO-1 and CD146 have been associated with MSC stemness [ 20 , 21 ]. STRO-1 + or CD146 + subpopulation from human dental pulp stem cells (DPSCs) form more colony-forming units-fibroblastic (CFUs-F) than the heterogeneous pool of DPSCs [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Properties of Human ALP+ Periodontal Ligament Stem Cells vs Their ALP- Counterparts

Gauthier

Tran

et al. 2015

J Stem Cell Res Ther

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Characterizing subpopulations of stem cells is important to understand stem cell properties. Tissue-nonspecific alkaline phosphatase (ALP) is associated with mineral tissue forming cells as well as stem cells. Information regarding ALP subpopulation of human periodontal ligament stem cells (hPDLSCs) is limited. In the present study, we examined ALP+ and ALP− hPDLSC subpopulations, their surface markers STRO-1 and CD146, and the expression of stemness genes at various cell passages. We found that ALP+ subpopulation had higher levels of STRO-1 (30.6 ± 5.6%) and CD146 (90.4 ± 3.3%) compared to ALP− (STRO-1: 0.5 ± 0.1%; CD146: 75.3 ± 7.2%). ALP+ cells expressed significantly higher levels of stemness associated genes, NANOG, OCT4 and SOX than ALP− cells at low cell passages of 2-3 (p<0.05). ALP+ and ALP− cells had similar osteogenic, chondrogenic and neurogenic potential while ALP−, not ALP+ cells, lacked adipogenic potential. Upon continuous culturing and passaging, ALP+ continued to express higher stemness genes and STRO-1 and CD146 than ALP− cells at ≥passage 19. Under conditions (over-confluence and vitamin C treatment) when ALP+ subpopulation was increased, the stemness gene levels of ALP+ was no longer significantly higher than those in ALP− cells. In conclusion, ALP+ hPDLSCs possess differential properties from their ALP− counterparts.

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Bone Marrow Mesenchymal Stem Cells

Cited by 3 publications

References 102 publications

Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D‐printed scaffolds

Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D‐printed scaffolds

Investigation of gene expressions in differentiated cell derived bone marrow stem cells during bone morphogenetic protein-4 treatments with Fourier transform infrared spectroscopy

Differential Properties of Human ALP+ Periodontal Ligament Stem Cells vs Their ALP- Counterparts

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