Bone Marrow Seeded Bone Graft Versus Bone Graft; Compact Bone Critical Sized Defect Healing Pattern in Rabbit

Kaveh, Kamran; Ibrahim, Rashid; Ibrahim, Tengku Azmi Tengku; Zuki, A. B. Mohd

doi:10.3923/javaa.2010.1588.1596

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…In group C, intense new bone formation was found in the edge of defect area as compared to group B, where the osteogenesis was noticed in the middle of defect. This results correlated with the work of Kaveh (2010), in which they found that bone marrow seeded corticocancellous graft showed osteogenesis at the centre of graft, as compared to non seeded graft. Another study also suggested that bone synthesis in the middle region of implant was more pronounced in osteoblast containing processed bovine cancellous matrix in comparison to acellular (Kneser et al, 2006).…”

Section: Histological Observationssupporting

confidence: 88%

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Rashmi¹,

Pathak²,

Tamilmahan³

et al. 2020

IJAR

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The present work was assessed the restoration of segmental bone gap defect in rabbits by application of composite bone grafts. Composite bone grafts were prepared by seeding of rabbit foetal osteoblast on the bovine acellular cancellous bone matrix and evaluated in the segmental gap defect of 20 mm in rabbits. Thirty six adult New Zealand White rabbits of either sex were allotted to three groups of 12 each. Autograft (group A), Acellular cancellous bone matrix (group B) and composite graft seeded with fetal osteoblasts (group C) were implanted in the defects. Radiography, gross observations and histopathology at different intervals were done to evaluate healing. It was concluded that the seeded foetal osteoblasts in the composite grafts augment regeneration of the new bone leading to better integration of graft in host in comparison to bovine acellular cancellous bone matrix graft.

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Section: Histological Observationssupporting

confidence: 88%

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Rashmi¹,

Pathak²,

Tamilmahan³

et al. 2020

IJAR

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Mesenchymal Stem Cells of Different Origin-Seeded Bioceramic Construct in Regeneration of Bone Defect in Rabbit

Maiti

Shivakumar

Mohan

et al. 2018

Tissue Eng Regen Med

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Bone marrow derived mesenchymal stem cells (BMSC) represent an attractive cell population for tissue engineering purpose. The objective of this study was to determine whether the addition of recombinant human bone morphogenetic protein (rhBMP-2) and insulin-like growth factor (IGF-1) to a silica-coated calcium hydroxyapatite (HASi) - rabbit bone marrow derived mesenchymal stem cell (rBMSC) construct promoted bone healing in a large segmental bone defect beyond standard critical -size radial defects (15mm) in rabbits. An extensively large 30mm long radial ostectomy was performed unilaterally in thirty rabbits divided equally in five groups. Defects were filled with a HASi scaffold only (group B); HASi scaffold seeded with rBMSC (group C); HASi scaffold seeded with rBMSC along with rhBMP-2 and IGF-1 in groups D and E respectively. The same number of rBMSC (five million cells) and concentration of growth factors rhBMP-2 (50µg) and IGF-1 (50µg) was again injected at the site of bone defect after 15 days of surgery in their respective groups. An empty defect served as the control group (group A). Radiographically, bone healing was evaluated at 7, 15, 30, 45, 60 and 90 days post implantation. Histological qualitative analysis with microCT (µ-CT), haematoxylin and eosin (H & E) and Masson’s trichrome staining were performed 90 days after implantation. All rhBMP-2-added constructs induced the formation of well-differentiated mineralized woven bone surrounding the HASi scaffolds and bridging bone/implant interfaces as early as eight weeks after surgery. Bone regeneration appeared to develop earlier with the rhBMP-2 constructs than with the IGF-1 added construct. Constructs without any rhBMP-2 or IGF-1 showed osteoconductive properties limited to the bone junctions without bone ingrowths within the implantation site. In conclusion, the addition of rhBMP-2 to a HASi scaffold could promote bone generation in a large critical-size-defect.

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Engineered Fe(OH)₃ nanoparticle-coated and rhBMP-2-releasing PLGA microsphere scaffolds for promoting bone regeneration by facilitating cell homing and osteogenic differentiation

Zhang

Lin

et al. 2018

J. Mater. Chem. B

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Bone Marrow Seeded Bone Graft Versus Bone Graft; Compact Bone Critical Sized Defect Healing Pattern in Rabbit

Cited by 3 publications

References 12 publications

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Mesenchymal Stem Cells of Different Origin-Seeded Bioceramic Construct in Regeneration of Bone Defect in Rabbit

Engineered Fe(OH)₃ nanoparticle-coated and rhBMP-2-releasing PLGA microsphere scaffolds for promoting bone regeneration by facilitating cell homing and osteogenic differentiation

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Bone Marrow Seeded Bone Graft Versus Bone Graft; Compact Bone Critical Sized Defect Healing Pattern in Rabbit

Cited by 3 publications

References 12 publications

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

Mesenchymal Stem Cells of Different Origin-Seeded Bioceramic Construct in Regeneration of Bone Defect in Rabbit

Engineered Fe(OH)3 nanoparticle-coated and rhBMP-2-releasing PLGA microsphere scaffolds for promoting bone regeneration by facilitating cell homing and osteogenic differentiation

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Engineered Fe(OH)₃ nanoparticle-coated and rhBMP-2-releasing PLGA microsphere scaffolds for promoting bone regeneration by facilitating cell homing and osteogenic differentiation