Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3,4-dihydroxy-L-phenylalanine (DOPA)

Abstract

The stability of graf t material and membrane is crucial for successf ul guided bone regeneration. As 3,4-dihydroxy-L-phenylalanine (DOPA) is known to contribute to the adhesiveness of natural mussel adhesive proteins, the aim of this study was to evaluate the biocompatibility and bone regeneration using collagenated synthetic bone graft attached to the collagen membrane with DOPA in the rabbit calvaria model. Materials and Methods:Four circular bone defects of 8 mm diameter were created in the rabbit calvarium. Each defect in the calvarium was randomly allocated to 1) sham control group, 2) bone graft using a particle-type synthetic bone substitute (OP) group, 3) bone graft using collagenated synthetic bone block (OC) group, or 4) bone graf t using the collagenated synthetic bone graf t attached to collagen membrane with DOPA (OC-DOPA) group. A total of 10 rabbits were sacrificed at either 2 or 8 weeks post-surgery.Results: During histometric analysis, new bone formation in the OC-DOPA group was significantly greater than that in sham control at 8 weeks. On the contrary, there was no statistically significant difference in new bone area (NBA) between OC-DOPA and OC. Histologic analysis revealed infiltrations of inflammatory cells in the OP, OC, and OC-DOPA groups at 2 weeks. At 8 weeks, there

Application of modified porcine xenograft by collagen coating in the veterinary field: pre-clinical and clinical evaluations

“…Bone graft materials usually have one or more components: an osteoconductive matrix to support new bone ingrowth, osteoinductive proteins to support mitogenesis of undifferentiated cells, and osteogenic cells (osteoblasts or osteoblast precursors) to form bone in the proper environment ( 11 ). In addition, materials that promote bone formation, such as membranes or recombinant human bone morphogenetic protein-2 (rhBMP-2), are continuously researched to improve bone grafting efficacy ( 12 , 13 ).…”

“…Due to this effect, methods of mixing collagen components with various bone graft materials have recently been continuously studied ( 25 ). In particular, several experimental studies have investigated mixing various bovine-derived bone graft materials or synthetic bone graft substitutes with collagen ( 12 , 21 , 26 ). Nevertheless, there has been relatively limited research on combining collagen with bone graft material derived from porcine bone ( 14 ), and its clinical applications are rare in veterinary medicine.…”