Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model

Klein, Yehuda; Fleissig, Omer; Stabholz, Adam; Chaushu, Stella; Polak, David

doi:10.1002/jper.17-0550

Cited by 23 publications

(44 citation statements)

References 43 publications

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“…A total of 12 mice were divided randomly into 1) test group in which the sockets were filled with bovine bone (BB + , n = 6) and 2) control group in which the sockets remained without graft filling (BB – , n = 6). All mice were sacrificed after 7 wk based on our previous study (Klein, Fleissig, et al 2019), which showed full osseous healing at 4 wk after bone regeneration.…”

Section: Methodsmentioning

confidence: 99%

“…Bovine bone was pulverized coarsely with a sterile mortar and pestle as we previously described (Klein, Fleissig, et al 2019). The bovine bone particles size was measured under light microscopy with a mean diameter of 56.07 ± 29.46 µm and stored at room temperature until use.…”

Section: Methodsmentioning

confidence: 99%

“…All experimental protocols followed ARRIVE (Animal Research Reporting of In Vivo Experiments) guidelines and were approved by the Animal Care and Use Committees of the Hebrew University. The ABR model in mouse was performed as we previously described (Klein, Fleissig, et al 2019; Klein, Kunthawong, et al 2019). In brief, mice were generally anesthetized with intraperitoneal injection of ketamine (200 mg/kg) and xylazine (10 mg/kg) at a 9:1 ratio, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Maxillae samples underwent µCT scan at high resolution (10 µm isotropic nominal resolution), 70 kV energy, 114 µA intensity, and 1,000 projection at a 200-ms integration time as we previously described (Klein, Fleissig, et al 2019; Klein, Kunthawong, et al 2019). The borders of the regenerated bone sites were marked according to a cylindrical region of interest (ROI) in all the samples mesial to M2 with an axis depth/length of 350 µm (150 to 500 µm below the M2 root furcation) and a diameter of 700 µm.…”

Section: Methodsmentioning

confidence: 99%

“…Tovar et al (2014) demonstrated bone formation in critical defects filled with bovine bone in a rabbit calvaria model, exhibiting osseous healing with full osseous integration between the bovine bone particles and the newly formed bone with no adverse tissue response. A more recent study showed, in a mouse first molar extraction socket model, full histological and radiographical bone healing at 4 wk after bovine bone grafting (Klein, Fleissig, et al 2019). No significant changes in the bone parameters were subsequently observed, and resorption of bone particles was not significant during the whole (7-wk) experimental period.…”

Section: Introductionmentioning

confidence: 99%

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Bovine Bone Promotes Osseous Protection via Osteoclast Activation

et al. 2020

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The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model ( n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group ( n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group ( n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts’ function. Osteoclasts’ inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts’ inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine Bone Promotes Osseous Protection via Osteoclast Activation

et al. 2020

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Biomimetic Periodontal Ligament Transplantation Activated by Gold Nanoparticles Protects Alveolar Bone

Zong

Holm

Bronckaers

et al. 2023

Adv Healthcare Materials

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Stem cell therapy might be a promising method to stimulate alveolar bone regeneration, which is currently a major clinical challenge. However, its therapeutic features largely depend on pretreatment and transplantation preparation. Herein, a novel biomimetic periodontal ligament transplantation composed of human periodontal ligament stem cells (hPDLSCs) pretreated with gold nanocomplexes (AuNCs) and embedded in a type-I collagen hydrogel scaffold is developed to protect alveolar bone from resorption. AuNCs are readily absorbed by primary hPDLSCs, with limited cytotoxicity, and promote osteogenic differentiation of hPDLSCs effectively in vitro. In addition, the AuNCs-induced hPDLSCs are encapsulated with type-I collagen hydrogel scaffold to mimic their native physiological niche, and then are transplanted into a rat model of alveolar bone resorption. Both micro-computed tomography (micro-CT) and immunohistochemical assays demonstrate that alveolar bone loss is significantly prevented. Furthermore, the underlying therapeutic mechanism is elucidated, in which transplantation-activated osteogenesis is associated with autophagy, which enables bone remodeling and regeneration. This study provides critical insight into the role of PDLSCs in bone homeostasis and proposes an innovative AuNCs-based strategy for stem cell therapy in bone regeneration.

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Development and regeneration of periodontal supporting tissues

Guo

Bai

Wang

et al. 2022

Genesis

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Summary Periodontal tissues, including gingiva, cementum, periodontal ligament, and alveolar bone, play important roles in oral health. Under physiological conditions, periodontal tissues surround and support the teeth, maintaining the stability of the teeth and distributing the chewing forces. However, under pathological conditions, with the actions of various pathogenic factors, the periodontal tissues gradually undergo some irreversible changes, that is, gingival recession, periodontal ligament rupture, periodontal pocket formation, alveolar bone resorption, eventually leading to the loosening and even loss of the teeth. Currently, the regenerations of the periodontal tissues are still challenging. Therefore, it is necessary to study the development of the periodontal tissues, the principles and processes of which can be used to develop new strategies for the regeneration of periodontal tissues. This review summarizes the development of periodontal tissues and current strategies for periodontal healing and regeneration.

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Bone regeneration with bovine bone impairs orthodontic tooth movement despite proper osseous wound healing in a novel mouse model

Abstract: The two novel mouse models show that the lack of resorption of BB xenografts renders them inadequate for proper orthodontic tooth movement at a later stage.

Cited by 23 publications

References 43 publications

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

Biomimetic Periodontal Ligament Transplantation Activated by Gold Nanoparticles Protects Alveolar Bone

Development and regeneration of periodontal supporting tissues

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