Bone Remodeling Biomarkers of Periodontal Disease in Saliva

Frodge, Brandon D.; Ebersole, Jeffrey L.; Kryscio, Richard J.; Thomas, Mark V.; Miller, Craig S.

doi:10.1902/jop.2008.080070

Cited by 115 publications

(129 citation statements)

References 70 publications

(96 reference statements)

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“…The level of β-glucuronidase, which signals the influx of neutrophils, was confirmed in correlation with the increasing severity of periodontitis (28). Similarly, this correlation was confirmed in IL-1β, an important pro-inflammatory cytokine that is the predominant form of periodontitis (two forms: IL-1 alpha and IL-1 beta) and also in TNF-α (7,8,48). The newly identified anti-inflammatory interleukin IL-35, which is a member of the IL-12 family, is secreted by regulatory T-cells and suppresses the inflammatory response of immune cells.…”

Section: Markers Of Inflammationmentioning

confidence: 67%

“…For the purpose of threshold determination for periodontitis, several combinations of parameters were used; in one case the combination of MMP-8 and IL-1β showed an association with a significantly higher risk for periodontal disease. Combinations of these parameters also more often exhibit a positive predictive value for confirmation of the disease (36,8).…”

Section: Creating Of Diagnostic Panel For Periodontitismentioning

confidence: 99%

“…Longitudinal studies would be especially suitable for patients with aggressive periodontitis (36). The main problem with these biomarkers lies in their extremely low concentrations at the time of remission and at the time of exacerbation of premature degradation in saliva (8). The most important such biomarkers are alkaline phosphatase (ALP), C-terminal telopeptide (carboxy-terminal collagen crosslinks or CTX) and a recent receptor activator of nuclear factor kappa-B ligand (receptor activator of NF-κB or RANKL) and osteoprotegerin (OPG).…”

Section: Markers Of Bone Remodellingmentioning

confidence: 99%

“…2). All the reagents are present on the chip; the temperature is controlled 8 by thermal chamber, and the individual reagents are placed in a wax which melts during the initial heating and reagents are hydrated in the kit. The amplification process can be observed in real time, when using an appropriate colour, e.g.…”

Section: Recent Trends In the Diagnosis Of Periodontal Diseasesmentioning

confidence: 99%

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Trends in Laboratory Diagnostic Methods in Periodontology

Bolerázska

Mareková

Markovská

2016

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary: This work presents a summary of current knowledge on the laboratory diagnosis of periodontitis. It focuses on the theoretical foundations and is supplemented with new knowledge. It subsequently describes specifically the laboratory diagnosis methods of periodontitis: the protein expression of inflammation, oral microbiology and molecular diagnostics. Periodontitis is a serious disease worldwide and its confirmed association with systemic diseases means its severity is increasing. Its laboratory diagnosis has the potential to rise to the level of clinical and diagnostic imaging. The transfer of diagnostic methods from laboratory to clinical use is increasingly used in the prevention and monitoring of the exacerbation and treatment of periodontal disease, as well as of its impact on systemic disease.

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Section: Markers Of Inflammationmentioning

confidence: 67%

Section: Creating Of Diagnostic Panel For Periodontitismentioning

confidence: 99%

Section: Markers Of Bone Remodellingmentioning

confidence: 99%

Section: Recent Trends In the Diagnosis Of Periodontal Diseasesmentioning

confidence: 99%

See 2 more Smart Citations

Trends in Laboratory Diagnostic Methods in Periodontology

Bolerázska

Mareková

Markovská

2016

Acta Med. (Hradec Kralove, Czech Repub.)

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“…In a case-control study, Frodge et al evaluated 35 patients with moderate to severe periodontitis and 39 healthy controls to determine the correlation between periodontitis and bone remodeling biomarkers in saliva [13]. Tumor necrosis factoralpha (TNF-alpha), C-telopeptide pyridinoline cross-links of type I collagen (ICTP), and receptor activator of nuclear factor-kappa B ligand (RANKL) were evaluated, and it was determined that salivary levels of TNF-alpha only were significantly higher in individuals with periodontal disease.…”

Section: Diagnosis-salivary Biomarkersmentioning

confidence: 99%

Systemic Biomarkers for Periodontitis

Stathopoulou

Buduneli

Kinane

2015

Curr Oral Health Rep

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The interaction between serum amyloid A and Toll‐like receptor 2 pathway regulates inflammatory cytokine secretion in human gingival fibroblasts

Song

Lai

et al. 2019

Journal of Periodontology

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Background Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis Methods SAA mRNA and protein expression were detected in healthy and inflammatory gingival tissues using real‐time polymerase chain reaction (PCR) and immunohistochemistry. Human recombinant SAA (Apo‐SAA), Pam3CSK4 (a Toll‐like receptor (TLR) 2 ligand), siRNA‐SAA, or TLR2 neutralizing antibody was applied to treat human gingival fibroblasts, respectively, or combined. SAA, TLRs, and inflammatory cytokines interleukin (IL)‐6 and IL‐8 were analyzed by real‐time PCR, western blotting, or enzyme‐linked immunosorbent assay. Results SAA expression increased in human inflammatory gingival tissues from patients with periodontitis (P <0.05). Apo‐SAA could increase not only the mRNA expression of TLR2 (P <0.05), but also IL‐6 and IL‐8 mRNA and protein levels (P <0.05) which was suppressed by TLR2 antibody in human gingival fibroblasts. Pam3CSK4 increased SAA, IL‐6, and IL‐8 levels (P <0.05). However, the expression of SAA, IL‐6, and IL‐8 decreased after transfection of siRNA‐SAA (P <0.05). Conclusion SAA not only increases in inflammatory gingiva, but also triggers inflammatory cytokine secretion via interacting with TLR2 pathway in human gingival fibroblasts, which indicates that SAA is involved in periodontal inflammation.

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Bone Remodeling Biomarkers of Periodontal Disease in Saliva

Abstract: Salivary levels of TNF-alpha were elevated in patients who had clinical indicators of periodontitis, suggesting that this biomarker may serve in a panel of salivary biomarkers that could facilitate the screening, diagnosis, and management of periodontal disease.

Cited by 115 publications

References 70 publications

Trends in Laboratory Diagnostic Methods in Periodontology

Trends in Laboratory Diagnostic Methods in Periodontology

Systemic Biomarkers for Periodontitis

The interaction between serum amyloid A and Toll‐like receptor 2 pathway regulates inflammatory cytokine secretion in human gingival fibroblasts

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