Bone tissue engineering

Ursino, Heather L.; James, Bryan D.; Ludtka, Christopher; Allen, Josephine B.

doi:10.1016/b978-0-12-820508-2.00018-0

Cited by 4 publications

(3 citation statements)

References 334 publications

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“…90 This response can be examined in the form of changes in bone structure such as osteoclast cell migration, accelerated bone formation by osteoblasts, and an increase in osteopontin concentration. 91 Since ABGs must convert mechanical pressure into electrical force in order to stimulate ossifying cells through the movement of bone fluid, the 3D protein network along with the integrated structures of the ABG bone channels must be protected from destruction. 92 As a result, a more accurate estimation of the tissue healing process can be provided by investigating the ability to convert mechanical pressure to chemical or electrical signals and its distribution in ABGs.…”

Section: Abgs Function Evaluation Indicatorsmentioning

confidence: 99%

“…Although there are some questions about how mechanical stimulation works and what role it plays in bone tissue, various reports suggest that bone cells receive mechanical force and respond to it via chemical or electrical signals . This response can be examined in the form of changes in bone structure such as osteoclast cell migration, accelerated bone formation by osteoblasts, and an increase in osteopontin concentration . Since ABGs must convert mechanical pressure into electrical force in order to stimulate ossifying cells through the movement of bone fluid, the 3D protein network along with the integrated structures of the ABG bone channels must be protected from destruction .…”

Section: Bone Tissue Regenerationmentioning

confidence: 99%

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Criteria, Challenges, and Opportunities for Acellularized Allogeneic/Xenogeneic Bone Grafts in Bone Repairing

Sharifi

Kheradmandi

Salehi

et al. 2022

ACS Biomater. Sci. Eng.

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As bone grafts become more commonly needed by patients and as donors become scarcer, acellularized bone grafts (ABGs) are becoming more popular for restorative purposes. While autogeneic grafts are reliable as a gold standard, allogeneic and xenogeneic ABGs have been shown to be of particular interest due to the limited availability of autogeneic resources and reduced patient well-being in long-term surgeries. Because of the complete similarity of their structures with native bone, excellent mechanical properties, high biocompatibility, and similarities of biological behaviors (osteoinductive and osteoconductive) with local bones, successful outcomes of allogeneic and xenogeneic ABGs in both in vitro and in vivo research have raised hopes of repairing patients' bone injuries in clinical applications. However, clinical trials have been delayed due to a lack of standardized protocols pertaining to acellularization, cell seeding, maintenance, and diversity of ABG evaluation criteria. This study sought to uncover these factors by exploring the bone structures, ossification properties of ABGs, sources, benefits, and challenges of acellularization approaches (physical, chemical, and enzymatic), cell loading, and type of cells used and effects of each of the above items on the regenerative technologies. To gain a perspective on the repair and commercialization of products before implementing new research activities, this study describes the differences between ABGs created by various techniques and methods applied to them. With a comprehensive understanding of ABG behavior, future research focused on treating bone defects could provide a better way to combine the treatment approaches needed to treat bone defects.

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Section: Abgs Function Evaluation Indicatorsmentioning

confidence: 99%

Section: Bone Tissue Regenerationmentioning

confidence: 99%

Criteria, Challenges, and Opportunities for Acellularized Allogeneic/Xenogeneic Bone Grafts in Bone Repairing

Sharifi

Kheradmandi

Salehi

et al. 2022

ACS Biomater. Sci. Eng.

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“…Biomimetic collagen‐based scaffolds that are compositionally similar to native bone have demonstrated immense promise for BTE applications 8,9 . However, weak mechanical properties and susceptibility to expedited enzymatic degradation are major concerns with the use of collagen‐based scaffolds 10 .…”

Section: Introductionmentioning

confidence: 99%

A comparative study of bone bioactivity and osteogenic potential of different bioceramics in methacrylated collagen hydrogels

Patrawalla

Kajave

Kishore

2022

J Biomedical Materials Res

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Biomimetic scaffolds composed of bioactive ceramic‐based materials incorporated within a polymeric framework have shown immense promise for use in bone tissue engineering (BTE) applications. However, studies on direct comparison of the efficacy of different bioceramics on bone bioactivity and osteogenic differentiation are lacking. Herein, we performed an in vitro direct comparison of three different bioceramics—Bioglass 45S5 (BG), Laponite XLG (LAP), and β‐Tricalcium Phosphate (TCP)—on the physical properties and bone bioactivity of methacrylated collagen (CMA) hydrogels (10% w/w bioceramic:CMA). In addition, human MSCs (hMSCs) were encapsulated in bioceramic‐laden CMA hydrogels and the effect of different bioceramics on osteogenic differentiation of hMSCs was investigated in two different culture medium—osteoconductive (without dexamethasone [DEX]) and osteoinductive (with DEX). Results showed that the stability of CMA hydrogels was maintained upon bioceramic addition. Compression testing revealed that BG incorporation significantly decreased (p < 0.05) the modulus of photochemically crosslinked CMA hydrogels. Incubation of TCP‐CMA and LAP‐CMA hydrogels in simulated body fluid showed deposition of hydroxycarbonate apatite layer on the surface indicating that these hydrogels may be more bone bioactive than BG‐CMA and CMA only hydrogels. Cell cytoskeleton staining results showed greater cell spreading in TCP‐CMA hydrogels. Furthermore, TCP incorporation significantly increased alkaline phosphatase activity (ALP; p < 0.05) in hMSCs. Together, these results indicate that TCP has superior osteogenic potential compared with BG and LAP and hence should be considered as a bioceramic of preferred choice for use in the biomimetic design of cell‐laden hydrogels for BTE applications.

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Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation

Pipis,

Duraivel,

Subramaniam

et al. 2024

Regen. Eng. Transl. Med.

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Purpose Nucleic acid-collagen complexes (NACCs) are unique biomaterials formed by binding short, monodisperse single-stranded DNA (ssDNA) with type I collagen. These complexes spontaneously generate microfibers and nanoparticles of varying sizes, offering a versatile platform with potential applications in tissue engineering and regenerative medicine. However, the detailed mechanisms behind the nucleic acid-driven assembly of collagen fibers still need to be established. We aim to understand the relationship between microscopic structure and bulk material properties and demonstrate that NACCs can be engineered as mechanically tunable systems. Methods We present a study to test NACCs with varying molar ratios of collagen to random ssDNA oligonucleotides. Our methods encompass the assessment of molecular interactions through infrared spectroscopy and the characterization of gelation and rheological behavior. We also include phase contrast, confocal reflectance, and transmission electron microscopy to provide complementary information on the 3D structural organization of the hydrogels. Results We report that adding DNA oligonucleotides within collagen robustly reinforces and rearranges the hydrogel network and accelerates gelation by triggering rapid fiber formation and spontaneous self-assembly. The elasticity of NACC hydrogels can be tailored according to the collagen-to-DNA molar ratio, ssDNA length, and collagen species. Conclusion Our findings hold significant implications for the design of mechanically tunable DNA-based hydrogel systems. The ability to manipulate hydrogel stiffness by tailoring DNA content and collagen concentration offers new avenues for fine tuning material properties, enhancing the versatility of bioactive hydrogels in diverse biomedical applications. Lay Summary This work is an example of forming fibers and gels with tunable elasticity that stems from the complexation of short-length nucleic acids (on the order of size of aptamers) and collagen, which can be potentially extended to a variety of functionalized hydrogel designs and tailored biomedical applications. Incorporating DNA induces mechanical changes in NACCs. Graphical Abstract

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Bone tissue engineering

Cited by 4 publications

References 334 publications

Criteria, Challenges, and Opportunities for Acellularized Allogeneic/Xenogeneic Bone Grafts in Bone Repairing

Criteria, Challenges, and Opportunities for Acellularized Allogeneic/Xenogeneic Bone Grafts in Bone Repairing

A comparative study of bone bioactivity and osteogenic potential of different bioceramics in methacrylated collagen hydrogels

Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation

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