Bone tissue engineering on amorphous carbonated apatite and crystalline octacalcium phosphate-coated titanium discs

Dekker, R; Bruijn, Joost D. de; Stigter, M.; Barrère, Florence; Layrolle, Pierre; Blitterswijk, Clemens A. van

doi:10.1016/j.biomaterials.2005.01.057

Cited by 111 publications

(80 citation statements)

References 42 publications

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“…Recently, synthetic OCP has been used as a bone substitute in tissue engineering for bone regeneration [3][4][5][6][7] . Much attention has been paid to the usability of synthetic OCP and its predicted role in nucleation of bone induction in orthotopic sites, which can be replaced with a significantly higher volume of newly formed bone than the other calcium phosphate phases, such as amorphous carbonated apatite or HA 8) . The osteoconductivity of OCP was first found in the granule form on implantation in the subperiosteal region of murine calvaria 9,10) .…”

Section: Introductionmentioning

confidence: 99%

“…The osteoconductivity of OCP was first found in the granule form on implantation in the subperiosteal region of murine calvaria 9,10) . The biological responses to OCP have also been investigated from its coating on metallic implants 11,12) , in osteoblastic cell proliferation, or from implantation into subcutaneous tissue or bone defects 8,12) . Recent studies suggested that osteoblastic cell differentiation is enhanced upon contact with OCP, which tends to convert into HA 13) , and differentiation is significantly facilitated in an OCP dose-dependent manner 14) .…”

Section: Introductionmentioning

confidence: 99%

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Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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“…After subcutaneously implanted in nude mice for 4 week, clear de novo bone formation was observed on all discs with cultured cells. Compared to the amorphous CA-coated discs, the newly formed bone on the crystalline OCP-coated discs was more organized and showed a significantly higher volume and the percentage of bone contact (Dekker et al, 2005). Yuan et al reported that OCP-coated porous tantalum implants induced bone formation after implantation in the dorsal muscles of adult dogs for 3 months, while the uncoated one did not (Yuan, 2001).…”

Section: Bone Tissue Engineering On Apatite-coated Titanium Discsmentioning

confidence: 99%

“…When biomimetic apatite-coated metal was implanted in vivo, they reacted dynamically towards the surrounding body fluids and showed a series of different biological behavior such as enhancing bone integration, inducing ectopic bone formation and combining with cultured bone marrow cells to inducing bone formation, which was closely related to the degradation behavior of the coating (Barrère et al, 2003a(Barrère et al, , 2003cDekker et al, 1998Dekker et al, , 2005Habibovic et al, 2005). In a simulated physiological solution CA and OCP coatings showed different dissolution rates.…”

Section: In Vitro and In Vivo Degradation Of Biomimetic Apatite Coatingmentioning

confidence: 99%

Advances in Biomimetic Apatite Coating on Metal Implants

Chaoyong¹,

Fan²,

Zhang³

2011

Advances in Biomimetics

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“…Por imersão em SBF 2, obteve-se um recobrimento com morfologia característica das fases amorfa de fosfato de cálcio e OCP, este último caracterizado por cristais orientados perpendicularmente semelhante a agulhas (acuniformes) 11,12,15 . Por DRX, observou-se somente a presença destas fases, Figura 3.…”

Section: Recobrimento Por Sbfunclassified

Estudo da influência dos íons K+, Mg2+, SO4(2-) e CO3(2-) na cristalização biomimética de fosfato de cálcio amorfo (ACP) e conversão a fosfato octacálcico (OCP)

Aparecida¹,

Fook²,

Santos³

et al. 2007

Quím. Nova

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OCP). The crystallization of hydroxyapatite (HA) in aqueous solution can be described by the mechanism ACP → OCP → HA. In this work, it was studied the influence of K + , Mg 2+ , SO 4 2-and CO 3 2-ions in the formation of ACP and in its conversion to OCP, using biomimetic coatings on metallic substrates of commercially pure titanium (Ti c.p.). The results showed that Mg 2+ and CO 3 2-ions favored both the formation of ACP and its conversion to OCP. Differently, K + and SO 4 2-ions did not influence the formation of ACP and, consequently, interfered in the conversion to OCP.Keywords: biomimetic coating; amorphous calcium phosphate; octacalcium phosphate. INTRODUÇÃODurantes as décadas passadas, as biocerâmicas de fosfato de cál-cio têm sido amplamente utilizadas em aplicações médicas, ortopédi-cas e odontológicas, como recobrimentos ou materiais densos para a reposição e reparação do tecido ósseo, devido à similaridade química e estrutural com a apatita biológica, que propicia a interação direta com o tecido ósseo [1][2][3][4] . Uma forma conveniente de classificar os fosfatos de cálcio é através da razão molar entre os átomos de cálcio e fósforo -Ca/P, a qual varia de 0,5 a 2,0 conforme mostra a Tabela 1. Os fosfatos de cálcio podem ser sintetizados por precipitação a partir de soluções contendo íons Ca 2+ e PO 4 3-, sob condições alcalinas ou ácidas. A hidroxiapatita -HA, por constituir o principal componente mineral dos ossos, é o fosfato de cálcio mais utilizado como biocerâmica, seja como recobrimento ou material denso 1 . Contudo, além da HA, vários outros fosfatos de cálcio também ocorrem em calcificações normais e patológicas, o que vem despertando interesse significativo nas possibilidades de utilização destes materiais como biocerâmicas 5 . A Tabela 2 relaciona diversos fosfatos de cálcio e suas ocorrências em sistemas biológicos.A utilização de HA em recobrimentos tornou-se popular em implantes dentários e ortopédicos devido às seguintes vantagens: rápida adaptação óssea, não formação de tecido fibroso, íntima adesão implante/tecido, tempo de cicatrização reduzido e maior tolerância a imprecisões cirúrgicas. A estas vantagens podem estar associadas outras características, como um sistema de manutenção do osso ao redor da área recoberta que aumenta a tolerância às mudanças ósseas naturais inevitáveis 6 . Apesar de todas estas vantagens, o uso clínico da HA é limitado devido à sua lenta biodegradação. Estudos efetuados por longos períodos de tempo têm mostrado que a HA começa a ser reabsorvida gradualmente após 4 a 5 anos de implantação. A reabsorção é uma característica desejada para biomateriais nos quais o processo de degradação é concomitante com a reposição do osso em formação 5 .

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Bone tissue engineering on amorphous carbonated apatite and crystalline octacalcium phosphate-coated titanium discs

Cited by 111 publications

References 42 publications

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Advances in Biomimetic Apatite Coating on Metal Implants

Estudo da influência dos íons K+, Mg2+, SO4(2-) e CO3(2-) na cristalização biomimética de fosfato de cálcio amorfo (ACP) e conversão a fosfato octacálcico (OCP)

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