Boosting the properties of a fluorescent dye by encapsulation into halloysite nanotubes

Riela, Serena; Barattucci, Anna; Barreca, Davide; Campagna, Sebastiano; Cavallaro, Giuseppe; Massaro, Marina; Pizzolanti, Giuseppe; Salerno, Tania Maria Grazia; Bonaccorsi, Paola; Puntoriero, Fausto

doi:10.1016/j.dyepig.2020.109094

Cited by 22 publications

(22 citation statements)

References 36 publications

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“…The study of release kinetics of CURBO from the lumen of the nanotubes, at different time intervals in phosphatebuffered saline (PBS) at pH 7.4, revealed that after about 8h the release percentage is equal to 4.6% and that this remains constant after 24h. 28 Therefore, the HNT/CURBO…”

Section: Discussionmentioning

confidence: 99%

“…The study of release kinetics of CURBO from the lumen of the nanotubes, at different time intervals in phosphate-buffered saline (PBS) at pH 7.4, revealed that after about 8h the release percentage is equal to 4.6% and that this remains constant after 24h. 28 Therefore, the HNT/CURBO could be used as an efficient imaging agent for biological purposes. Furthermore, due to the localization of the fluorescent probe into the HNT lumen, the developed system can be similar to pristine HNT since its external surface is unmodified.…”

Section: Discussionmentioning

confidence: 99%

“…To this end, since nanotubes do not possess photo-luminescent properties, the hybrid system HNT/CURBO has been used, in which the fluorescent probe CURBO is supramolecularly loaded into the HNT lumen (Figure 1). 28 The peculiarity of this hybrid system is that the fluorescent probe has a great affinity for the lumen, so is very similar to pristine halloysite nanotubes because the external surface does not have any type of functionalization. This allowed us to study the interaction with the plasma membrane, without any influence that could be given by the addition of functional groups on the external surface.…”

Section: Introductionmentioning

confidence: 99%

Figure 1 Hybrid system HNT/CURBO obtained by supramolecular inclusion with the fluorescent probe CURBO in the lumen of the tubes. …”

Section: Introductionmentioning

confidence: 99%

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Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Biddeci¹,

Massaro²,

Riela³

et al. 2021

IJN

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Halloysite nanotubes (HNTs) are a natural aluminosilicate clay with a chemical formula of Al 2 Si 2 O 5 (OH) 4 ×nH 2 O and a hollow tubular structure. Due to their peculiar structure, HNTs can play an important role as a drug carrier system. Currently, the mechanism by which HNTs are internalized into living cells, and what is the transport pathway, is still unclear. Therefore, this study aimed at establishing the in vitro mechanism by which halloysite nanotubes could be internalized, using phagocytic and non-phagocytic cell lines as models. Methods: The HNT/CURBO hybrid system, where a fluorescent probe (CURBO) is confined in the HNT lumen, has been used as a model to study the transport pathway mechanisms of HNTs. The cytocompatibility of HNT/CURBO on cell lines model was investigated by MTS assay. In order to identify the internalization pathway involved in the cellular uptake, we performed various endocytosis-inhibiting studies, and we used fluorescence microscopy to verify the nanomaterial internalization by cells. We evaluated the haemolytic effect of HNT/CURBO placed in contact with human red blood cells (HRBCs), by reading the absorbance value of the supernatant at 570 nm. Results: The HNT/CURBO is highly biocompatible and does not have an appreciable haemolytic effect. The results of the inhibition tests have shown that the internalization process of nanotubes occurs in an energy-dependent manner in both the investigated cell lines, although they have different characteristics. In particular, in non-phagocytic cells, clathrin-dependent and independent endocytosis are involved. In phagocytic cells, in addition to phagocytosis and clathrin-dependent endocytosis, microtubules also participate in the halloysite cellular trafficking. Upon internalization by cells, HNT/CURBO is localized in the cytoplasmic area, particularly in the perinuclear region. Conclusion: Understanding the cellular transport pathways of HNTs can help in the rational design of novel drug delivery systems and can be of great value for their applications in biotechnology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Figure 1 Hybrid system HNT/CURBO obtained by supramolecular inclusion with the fluorescent probe CURBO in the lumen of the tubes. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Biddeci¹,

Massaro²,

Riela³

et al. 2021

IJN

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“…Halloysite clay is transparent and does not significantly change polymer composites' transparency [18]. Halloysite nanotubes boost the properties of fluorescent dyes by their encapsulation [19]. Functionalized halloysite has been fabricated as a novel hybrid clay mineral for dye removal.…”

Section: Introductionmentioning

confidence: 99%

Halloysite and Laponite Hybrid Pigments Synthesis with Copper Chlorophyll

et al. 2021

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Sustainable and green materials have been studied in dye and pigment productions to reduce their environment impacts from being produced and applied. Although natural dyes are an excellent choice to move from agrowaste, some improvements must be made before they are applied given their poor fastness. One way of improving natural dye properties is their adsorption into nanoclay structures to give hybrid pigments. This work used tubular halloysite and laminar laponite to adsorb and stabilize natural copper chlorophyll. With a statistical design of experiments, we observed interactions between synthesis factors, such as pH, ionic strength, and surfactant or silane modification. Cool hybrid pigments with high TSR (%) values and a wide color range were obtained by using dispersions with only distilled water at room temperature. Successful chlorophyll adsorption on both nanoclay surfaces took place by XRD and DTA analyses. The maximum natural dye absorption for both nanoclay types took place under acid conditions, pH 3–4, and in the presence of mordant. The TSR (%) improved by the silane pH interaction, and halloysite hybrid pigments obtained higher TSR values than the laponite ones. Finally, a wide chromatic green color range was obtained with the surfactant modification in both nanoclays, and the color fastening was also improved in the hybrid pigments application. The samples generated with 10% of hybrid pigments from both nanoclays and an Epoxy bioresin, show higher colorfastness than the sample with the natural chlorophyll, due to the nanoclays–dye interaction and protection.

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