Border Disease of Sheep. Comparison of the Results of Serological Testing using Complement Fixation, Immunodiffusion, Neutralization and Immunofluorescent Techniques

Huck, R.A.; Evans, Denise; Woods, D.G.; King, A. A.; Stuart, Patrick M.; Shaw, I. G.

doi:10.1016/s0007-1935(17)35238-7

Cited by 13 publications

(3 citation statements)

References 10 publications

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“…The detected seroprevalence may underestimate pestivirus activity in infected flocks. While the antibody has persisted for up to 485 days in ewes, infected when pregnant [10], it may become undetectable earlier in other sheep [28]. Out of the 91 flocks tested, 42 (46%) were found to contain sheep that were seropositive for pestivirus.…”

Section: Discussionmentioning

confidence: 99%

A survey of antibodies to pestivirus in sheep in the Republic of Ireland

O’Neill¹,

O’Connor²,

O’Reilly³

2004

Ir Vet J

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Sera from 1,448 adult ewes in 91 flocks, representing all 26 counties in the Republic of Ireland, were examined for pestivirus antibodies using a commercially available ELISA which detected IgG1 antibody to border disease virus. Eighty-one sheep (5.6%) in 42 flocks (46.0%) were antibody-positive. Within infected flocks, the mean seroprevalence level was 11.4% with a range of 6.3% to 30.0%. The highest antibody prevalence was detected in sheep from central lowland counties of Ireland. Comparative neutralisation testing of 42 ELISA-positive sera detected geometric mean antibody titres of 136 to the NADL strain of bovine viral diarrhoea virus (BVDV), 92 to the Moredun strain of border disease virus and 21 to the 137/4 strain of border disease virus. These results suggest that BVDV may be the major ruminant pestivirus infecting sheep in Ireland. Although there are high numbers of infected flocks, many sheep within such flocks remain antibody-negative and are at risk of giving birth to lambs with congenital border disease.

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Section: Discussionmentioning

confidence: 99%

A survey of antibodies to pestivirus in sheep in the Republic of Ireland

O’Neill¹,

O’Connor²,

O’Reilly³

2004

Ir Vet J

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“…LAMONT and STUART (10) showed the relationship of BD to SF, as well as to M D and BVD; these interrelationships have been further studied by HUCK et al (9). Development of antibodies to all of these viruses was not unexpected therefore, though it should bc pointed out that titres to SF were minimal.…”

Section: Discussionmentioning

confidence: 92%

Effects of Experimental Border Disease Infection in the Pregnant Sow

Wrathall

Bailey

Done

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe B

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Summary Three minimal disease sows were inoculated at 34 days' gestation with an emulsion of tissues from lambs affected with ovine border disease. The sows were killed at term (113 days) and their piglets were recovered alive by hysterectomy. No clinical abnormalities were seen in the sows following the inoculation, neither was there an unusual degree of fetal death. However, all 3 sows did develop antibodies to border disease and also to bovine virus diarrhoea and mucosal disease. Low titres to swine fever virus developed in 2 of the sows. Of 19 piglets which were born at term one had a small meningocoele associated with hypoplasia and malformation of the cerebellum; reduction in cerebellum weight was a feature in 9 other piglets also. An unusual feature of the neuro‐chemistry of the spinal cord was a decrease in phospholipid relative to cerebroside content. Zusammenfassung Effekt einer experimentellen Border Disease‐Infektion bei trächtigen Sauen Drei „minimal disease”‐Sauen wurden am 34. Tag der Trächtigkeit mit einer Gewebesuspension von Lämmern mit Border Disease inokuliert. Zum Zeitpunkt der Geburt (113 Tage) erfolgte die Tötung der Sauen und die Ferkel wurden lebend durch Hysterektomie gewonnen. Klinische Symptome wurden bei den Sauen nach der Inokulation nicht beobachtet. Auch fötale Todesfälle hielten sich in der Norm. Alle drei Sauen bildeten jedoch Antikörper gegen Border Disease‐Virus sowie gegen Virusdiarrhoe‐ und Mucosal Disease‐Virus, Niedrige Titer gegenüber Schweinepestvirus entwickelten sich bei 2 Sauen. Von den 19 geborenen Ferkeln hatte eins eine kleine Meningozoele, die mit Thypoplasie und Mißbildungen des Cerebellums einhergingen. Eine Reduktion des Gewichts des Cerebellums wurde bei weiteren 9 Ferkeln beobachtet. Ein ungewöhnlicher Befund bei der neurochemischen Untersuchung des Rückenmarks, was eine Verminderung der Phospholipide relativ zum Gehait an Cerebrosiden. Résumé Effet d'une infection expérimentale avec la maladie de Border chez des truies gestantes Trois truies gestantes de 34 jours ont été inoculées avec une suspension tissulaire d'agneaux atteints de maladie de Border. Les truies ont été sacrifiées à la fin de la gestation (113 jours) et les porcelets furent recueillis vivants par hysterectomie. On n'a pas observé de symptômes cliniques chez les truies après l'inoculation. La mortalité foetale est restée normale. Les trois truies formèrent des anticorps contre le virus de la maladie de Border, contre le virusdiarrhée et le virus de la maladie des muqueuses. Un titre bas contre le virus de la peste porcine s'est développé chez deux truies. Un porcelet sur 19 nés a manifesté une faible méningocèle accompagnée de thypoplasie et de malformations du cervelet. Une diminution du poids du cervelet a été constatée chez 9 autres porcelets. On a observé dans les examens neurochimiques de la moëlle épinière que les phospholipides étaient relativement diminués par rapport aux cérébrosides. Resumen Efecto de una infección de la enfermedad de la Border en cerdas gestantes Se inocularon tres ...

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“…Affected newborn lambs usually exibit an abnormally hairy birth coat, tremor associated with defective myelination of the central nervous system, and low viability (see 6 and 14). Since the antibodies induced in infected animals have been shown to cross-react with bovine viral diarrhea (BVD) and hog cholera (HC) viruses (7,11,16), the virus has been included in the pestivirus genus within the Togaviridae family (12). However more details of its antigenieity and physical properties must be known before it is placed in a definite taxonomic group.…”

Section: Introductionmentioning

confidence: 99%

Properties of Border disease virus as studied in a sheep cell line

Laude¹,

Gelfi²

1979

Archives of Virology

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A fetal lamb muscle cell line has been isolated in which two strains of Border disease virus replicate well and induce a clear-cut cytopathic effect, thus providing a sensitive and practical assay system for both the virus and neutralizing antibodies. This system enabled us to study some characteristics of Border disease virus in comparison with two other agents responsible for hog cholera and bovine viral diarrhea. Our results indicate that the last virus and the Border disease agent are indistinguishable in vitro.

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Border Disease of Sheep. Comparison of the Results of Serological Testing using Complement Fixation, Immunodiffusion, Neutralization and Immunofluorescent Techniques

Cited by 13 publications

References 10 publications

A survey of antibodies to pestivirus in sheep in the Republic of Ireland

A survey of antibodies to pestivirus in sheep in the Republic of Ireland

Effects of Experimental Border Disease Infection in the Pregnant Sow

Properties of Border disease virus as studied in a sheep cell line

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