Bordetella Adenylate Cyclase-Hemolysin Toxins

Guiso, Nicole

doi:10.3390/toxins9090277

Cited by 32 publications

(31 citation statements)

References 89 publications

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“…Five well-characterised virulence genes that thought to be involved in interactions with the host were screened in the isolates, including filamentous haemagglutinin (FHA) ( fhaB), pertactin ( prn), adenylate cyclase-haemolysin toxin (cyaA), dermonecrotic toxin (dnt) and the Bordetella type-III secretion system effector A (bteA) [15][16][17][18][19]. The primers for amplification of the five virulence genes were designed based on the corresponding genes in the NCBI database (https://www.ncbi.nlm.nih.gov/) ( Table 1).…”

Section: Virulence Gene Detectionmentioning

confidence: 99%

Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

et al. 2020

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Bordetella bronchiseptica is a potential zoonotic pathogen, which mainly causes respiratory diseases in humans and a variety of animal species. B. bronchiseptica is one of the important pathogens isolated from rabbits in Fujian Province. However, the knowledge of the epidemiology and characteristics of the B. bronchiseptica in rabbits in Fujian Province is largely unknown. In this study, 219 B. bronchiseptica isolates recovered from lung samples of dead rabbits with respiratory diseases in Fujian Province were characterised by multi-locus sequencing typing, screening virulence genes and testing antimicrobial susceptibility. The results showed that the 219 isolates were typed into 11 sequence types (STs) including five known STs (ST6, ST10, ST12, ST14 and ST33) and six new STs (ST88, ST89, ST90, ST91, ST92 and ST93) and the ST33 (30.14%, 66/219), ST14 (26.94%, 59/219) and ST12 (16.44%, 36/219) were the three most prevalent STs. Surprisingly, all the 219 isolates carried the five virulence genes (fhaB, prn, cyaA, dnt and bteA) in the polymerase chain reaction screening. Moreover, the isolates were resistant to cefixime, ceftizoxime, cefatriaxone and ampicillin at rates of 33.33%, 31.05%, 11.87% and 3.20%, respectively. This study showed the genetic diversity of B. bronchiseptica in rabbits in Fujian Province, and the colonisation of the human-associated ST12 strain in rabbits in Fujian Province. The results might be useful for monitoring the epidemic strains, developing preventive methods and preventing the transmission of epidemic strains from rabbits to humans.

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Section: Virulence Gene Detectionmentioning

confidence: 99%

Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

et al. 2020

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“…It should be aware that the PRN-deficient B. pertussis had emerged, and the losses of the prn gene expression in the strains might be driven by using of acellular vaccine containing PRN [14]. The cyaA gene encodes the multifunctional adenylate cyclasehemolysin (AC-Hly) toxin, which possess adenylate cyclase activity, pore-forming activity and cell invasive activity [15]. It was showed that the AC-Hly toxin expressed in the B. bronchiseptica strains isolated from human and rabbit was responsible for the lethality of the intranasally infected mice [7].…”

Section: Virulence Genes Detectionmentioning

confidence: 99%

Characterization of Bordetella Bronchiseptica Isolated from Rabbits in Fujian, China

Wang¹,

Sun

Chen

et al. 2020

Preprint

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Background Bordetella bronchiseptica can infect many animal species, and is a potential zoonotic pathogen that can also infect humans. In rabbits, infection of B. bronchiseptica is associated with respiratory disease, which causes economic losses to the rabbit farming. Fujian Province is a traditional importance rabbit farming area in China. However, no literature about the epidemiology and characteristics of B. bronchiseptica in rabbits in Fujian Province has been reported.Results A total of 219 B. bronchiseptica isolates were recovered from the 833 lung samples of dead rabbits with respiratory disease. The 219 isolates were typed into 11 sequence types (STs) including 5 known STs (ST6, ST10, ST12, ST14 and ST33) and 6 new STs (ST88, ST89, ST90, ST91, ST92 and ST93) by using multi-locus sequence typing (MLST). Surprisingly, all the 219 isolates carried the 5 virulence genes of fhaB, prn, cyaA, dnt and bteA in the PCR screening. Moreover, the isolates resistance to cefixime, ceftizoxime, cefatriaxone and ampicillin were detected, and the resistance rates to the 4 kinds of drug were 33.33, 31.05, 11.87 and 3.20%, respectively.Conclusions In the present study, we showed for the first time that B. bronchiseptica is widespread in rabbits in Fujian Province, and that B. bronchiseptica is an important pathogen associating with respiratory disease in rabbits in Fujian Province. Moreover, it should be alert to the potential occurrence of transmission events between rabbits and humans because the B. bronchiseptica strain of ST12 that can infect humans were also isolated from rabbits in Fujian Province.

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“…The adenylate cyclase (CyaA) toxin is a major virulence factor produced by Bordetella pertussis, the causative agent of whooping cough, and is involved in the early stages of respiratory tract colonization [1][2][3][4][5] . CyaA, a 1706-residue long protein ( Figure S1), is a Repeatin-ToXin (RTX) [6][7][8][9][10] multi-domain toxin [11][12] .…”

Section: Introductionmentioning

confidence: 99%

A high-affinity calmodulin-binding site in the CyaA toxin translocation domain is essential for invasion into eukaryotic cells

Voegele

Sadi

O’Brien

et al. 2020

Preprint

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The molecular mechanisms and forces involved in the translocation of bacterial toxins into host cells have thus far remained elusive. The adenylate cyclase (CyaA) toxin from Bordetella pertussis displays a unique intoxication pathway in which its catalytic domain is directly translocated across target cell membranes. We have previously identified a translocation region in CyaA that contains a segment, P454 (residues 454-484), exhibiting membrane-active properties related to antimicrobial peptides. Herein, we show that this peptide is able to translocate across membranes and interact with calmodulin. Structural and biophysical analyses have revealed the key residues of P454 involved in membrane destabilization and calmodulin binding. Mutational analysis demonstrated that these residues play a crucial role in CyaA translocation into target cells. We have also shown that calmidazolium, a calmodulin inhibitor, efficiently blocks CyaA internalization. We propose that after CyaA binding to target cells, the P454 segment destabilizes the plasma membrane, translocates across the lipid bilayer and binds calmodulin. Trapping of the CyaA polypeptide chain by the CaM:P454 interaction in the cytosol may assist the entry of the N-terminal catalytic domain by converting the stochastic process of protein translocation into an efficient vectorial chain transfer into host cells.

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Bordetella Adenylate Cyclase-Hemolysin Toxins

Cited by 32 publications

References 89 publications

Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

Characterization of Bordetella Bronchiseptica Isolated from Rabbits in Fujian, China

A high-affinity calmodulin-binding site in the CyaA toxin translocation domain is essential for invasion into eukaryotic cells

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