Boronic Acid‐containing Stimuli‐responsive Polymers Modified Nanopores for Label‐free Dual‐signal‐output Detection of Glucose

Abstract

Herein, a dual-signal-output glass nanopore system was developed for sensing glucose. Upon binding glucose, the boronic acid-containing stimuli-responsive polymer underwent a wettability switch and pKa shift. When the polymer was immobilized on the inner wall of nanopore, the nanopore offered a sensitive method for evaluating glucose by ion rectification. Besides, due to the electrostatic assembly of cationic pyrene onto the glucosebound anionic polymer, the pyrene excimer emission was observed. By separating the substrate binding and fluorescence reporting process, this work avoided the fluorescence labeling of the target and nanopore, thus simplifying the design of dual-signal-output nanopore for potential point-of-care test.

Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

“…Due to the electrostatic assembly of cationic pyrene onto the glucose-bound anionic polymer, the pyrene excimer emission was observed. 87 In summary, this method provides instant dual-mode glucose detection.…”

Pyrene-based monomer-excimer dual response organosilicon polymer for the selective detection of 2,4,6-trinitrotoluene (TNT) and 2,4,6-trinitrophenol (TNP)

“…), chemistry (e.g., detection of cations, 7,8 anions, 9,10 pH, 11,12 nitro compounds, 13,14 etc. ), and materials (e.g., responsive materials, [15][16][17] optical materials, 18-20 etc.). The distinct difference between monomer (370-400 nm) and excimer fluorescence (420-600 nm) depends on the structure and the distance of the pyrene derivatives.…”