Both hydrolytic and transesterification activities of Penicillium expansum lipase are significantly enhanced in ionic liquid [BMIm][PF6]

Enzymatic biodiesel production: An overview of potential feedstocks and process development

“…Value-added byproducts By By--product product (Lai et al, 2012;Yang et al, 2010;Zhang et al, 2011) showed that the use of ionic liquids leads to a significantly increased conversion yield compared with the use of either a tertbutanol or solvent-free system with Penicillium expansum lipase.…”

Penicillium expansum lipase-catalyzed production of biodiesel in ionic liquids

“…The superior conversion obtained in this IL, as compared to that obtained in the commonly used organic solvents (such as tert-butanol and hexane) and in the solvent-free system, has successfully demonstrated that the IL is a promising reaction medium for PEL-catalyzed biodiesel production. Our previous experimental results (Yang et al, 2010) have also suggested that direct addition of salt hydrates in the nonaqueous reaction systems affects the hydrolytic activity of PEL via a dual function: the water-buffering effect and the specific ion (Hofmeister) effect, and the latter seems to predominate in the IL system.…”

“…Previously, this enzyme has been utilized in catalyzing biodiesel production from corn oil (Li et al, 2007) and waste oil (Li et al, 2009) in organic media. Our recent study (Yang et al, 2010) investigated the catalytic activity of this enzyme in both ionic liquid and organic solvent systems and first explored its use in catalyzing the methanolysis of corn oil in the ionic liquid [BMIm] [PF 6 ]. The superior conversion obtained in this IL, as compared to that obtained in the commonly used organic solvents (such as tert-butanol and hexane) and in the solvent-free system, has successfully demonstrated that the IL is a promising reaction medium for PEL-catalyzed biodiesel production.…”

Use of ionic liquids in biodiesel production: a review

“…Despite the absence of enzymatic denaturation, removal of the byproduct triacetylglicerol is a disadvantage because the phase separation is not appropriate. Yang et al (2010) ]). Currently, 250 grams of these organic salts cost US$ 493 and US$ 590, respectively, in suppliers such as Sigma-Aldrich and Acros Organics.…”