Bothrops alternatus ENVENOMING IN YOUNG DOGS

Pérez, O. Acosta de; Koscinczuk, P.; Flinta, S. M.; Maidana, H. R.; Negrette, M. Sánchez

doi:10.1590/s0104-79301997000100006

Cited by 7 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pitvipers of the genus Bothrops spp. are responsible for 90% of envenomation by snakes in Brazil and the bites by the species B. alternatus are the most frequent snake accidents in Argentina …”

Section: Introductionmentioning

confidence: 99%

The Venomics of Bothrops alternatus is a Pool of Acidic Proteins with Predominant Hemorrhagic and Coagulopathic Activities

Ohler

Georgieva²,

Seifert³

et al. 2010

J. Proteome Res.

View full text Add to dashboard Cite

The venom proteome of Bothrops alternatus, a venomous snake widespread in South America, was analyzed by 2-D electrophoresis followed by mass spectrometric analysis and determination of enzymatic activities. The venomic composition revealed that metallo- and serine proteinases play primary roles in the pathogenesis of the envenomation by this pitviper. The identified 100 venom components with molecular masses from 10 to 100 kDa belong to six protein families: metalloproteinases, serine/thrombin-like proteinases, phospholipases A(2), L-amino acid oxidases, disintegrins and thrombin inhibitors. Metalloproteinases predominate and belong exclusively to the P-III class including the most potent hemorrhagic toxins. They represent 50% of all identified proteins. Two isoforms were identified: homologous to jararhagin, a hemorrhagic toxin, and to beritractivase, a nonhemorrhagic and pro-coagulant metalloproteinase. The B. alternatus venom is a rich source of proteins influencing the blood coagulation system with a potential for medical application. The isoelectric points of the components are distributed in the acidic pH range (the pI values are between 4 and 7) and no basic proteins were detected.

show abstract

Section: Introductionmentioning

confidence: 99%

The Venomics of Bothrops alternatus is a Pool of Acidic Proteins with Predominant Hemorrhagic and Coagulopathic Activities

Ohler

Georgieva²,

Seifert³

et al. 2010

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…A moderada diminuição das proteínas plasmáticas totais foi, provavelmente, decorrente da hemorragia ou perda de plasma no edema. Os níveis de fibrinogênio estavam acentuadamente diminuídos, como no envenenamento em humanos (Barraviera 1999) e em cães (Pérez et al 1997). Este fenômeno pode ser explicado pela ação de componentes presentes nos dois venenos utilizados no presente estudo (Jararafibrase I e II, KN-BJ 1 e 2, TL-BJ 1, 2 e 3; Jararacussin I), que consomem parte do fibrinogênio circulante.…”

Section: Resultados Laboratoriaisunclassified

Envenenamento experimental por Bothropoides jararaca e Bothrops jararacussu em ovinos: aspectos clínico-patológicos e laboratoriais

et al. 2010

View full text Add to dashboard Cite

The purpose of this study was to establish the clinic-pathological and laboratory changes in sheep inoculated with Bothropoides jararaca and Bothrops jararacussu venom to provide subsidies for the differential diagnosis of snake bites. The liofilized venoms were diluted in 1 ml saline and administrated subcutaneously to four sheep. Three of the animals died, and the one that received 0.5mg/kg (B. jararaca venom) recovered. First symptoms were observed from 7 minutes to 1 hour after inoculation, and the clinical course varied from 7 hours and 9 minutes to 21 hours and 59 minutes. The symptoms, independent of the dosage, were swelling of the inoculation site, increased bleeding time and capillary filling, tachycardia, dyspnea, pale mucous membranes and diminished reaction to external stimuli. Laboratory tests revealed pronounced normocytic and normochromic anemia, trombocytopenia, slight reduction of fibrogen and total plasmatic protein, in two animals diminished hematocrit, besides pronounced increase of creatinaquinase and lactic dehydrogenase. At necropsy, the main findings at the inoculation site and adjacent tissues were extensive hemorrhages in the sheep inoculated with jararaca venom, and predominantly edema in the two animals inoculated with jararacussu venom. In two sheep which received jararacussu venom, acute pulmonary edema was observed. Hemorrhage and edema as the main histopathological changes, besides necrosis of muscle fibers and vessels at the inoculation site and adjacent tissue was observed. The renal tubular necrosis was attributed to shock. The volume increase at the inoculation site and surroundings was mainly due to hemorrhage (B. jararaca) or edema (B. jararacussu).

show abstract

“…O aumento da freqüência cardíaca e a queda da temperatura retal observados em todos os grupos podem ser explicados pela perda de sangue ocasionada pelo envenenamento botrópico. Diferente do observado neste trabalho, nos estudos de Pérez et al [19] realizados em 1997, os cães apresentaram bradicardia e dispnéia, podendo estes efeitos serem resultantes da dose de veneno de B. alternatus inoculada ser de 3mg/kg e da idade dos animais estar entre 30-65 dias, aumentando a severidade do envenenamento.…”

Section: Discussionunclassified

Avaliação clínica de cães tratados com flunixina meglumina, Curcuma longa e soro antibotrópico após envenenamento botrópico experimental (Bothrops alternatus)

Santos¹,

Melo²,

Jácome³

et al. 2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

show abstract

Bothrops alternatus ENVENOMING IN YOUNG DOGS

Cited by 7 publications

References 3 publications

The Venomics of Bothrops alternatus is a Pool of Acidic Proteins with Predominant Hemorrhagic and Coagulopathic Activities

The Venomics of Bothrops alternatus is a Pool of Acidic Proteins with Predominant Hemorrhagic and Coagulopathic Activities

Envenenamento experimental por Bothropoides jararaca e Bothrops jararacussu em ovinos: aspectos clínico-patológicos e laboratoriais

Avaliação clínica de cães tratados com flunixina meglumina, Curcuma longa e soro antibotrópico após envenenamento botrópico experimental (Bothrops alternatus)

Contact Info

Product

Resources

About