Botryosphaeran – A Fungal Exopolysaccharide of the (1→3)(1→6)-β-D-Glucan Kind: Structure and Biological Functions

Dekker, Robert F. H.; Queiroz, Eveline Aparecida Isquierdo Fonseca de; Cunha, Mário Antônio Alves da; Barbosa-Dekker, Aneli M.

doi:10.1007/978-3-030-12919-4_11

Cited by 17 publications

(6 citation statements)

References 169 publications

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“…The sorption equilibrium is dependent upon the pH as it affects the dissociation of cell wall carboxylic groups (pKa = 3.5–5.5), the content of which is higher as the pH increases, producing more free sites for the biosorption of lanthanide ions. As the pH increases, the concentration of H + ions decreases and positively charged lanthanide ions interact with the negatively charged binding sites on the microbial biomass . Our work found that acidic pH decreased the sorption capacity of the lanthanides in the highest proportions for the autoclaved cells showing only 17% of biosorption for La(III) and 4% for Sm(III) at pH 3.0.…”

Section: Resultsmentioning

confidence: 64%

“…B. rhodina MAMB‐05 was grown on sucrose and the mycelium was collected by centrifugation (1,500 g /15 min at 4°C) and freeze‐dried without prewashing the mycelium. The lyophilized fungal mycelium (LFM) was made into a powder in a blender at maximum speed, and stored dry in sealed plastic bags at −20°C until required.…”

Section: Methodsmentioning

confidence: 99%

“…In our work, we selected Botryosphaeria rhodina MAMB‐05 isolated from a eucalypt stem canker as a model fungus to study biosorption. This isolate produces a β‐D‐glucan of the (1→3)(1→6)‐linked type named botryosphaeran that presents several commercial applications. The structure and biological functions of botryosphaeran were recently reviewed .…”

Section: Introductionmentioning

confidence: 99%

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Biosorption of lanthanum and samarium by viable and autoclaved mycelium of Botryosphaeria rhodina MAMB‐05

Giese

Dekker

Barbosa-Dekker

2019

Biotechnology Progress

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The ascomycetous fungus, Botryosphaeria rhodina MAMB-05, secretes a (1!3)(1!6)β-D-glucan, and the scaled-up production of this β-glucan results in large amounts of mycelial biomass being produced that represent a potentially cost-effective biosorbent for rare-earth elements. High sorption capacities for both La(III) and Sm(III) were demonstrated for viable and autoclaved lyophilized fungal mycelium. Fourier-transformed infra-red absorption spectra and the point of zero charge (PZC) were similar for the viable and inactive fungal mycelia. The rare-earth lanthanide elements (La and Sm) binding increased at initial pH values greater than 5.0, which was also observed for the PZC determination. The maximum La(III) uptake capacity was observed at lower amounts of La(III) ions in solution, decreasing from 100.0 to 25.3% when the initial lanthanide concentration increased from 15 to 100 mg/L. Lanthanide biosorption by B. rhodina MAMB-05 mycelia followed the Langmuir model, and the affinity of biosorbent functional groups was similar for La(III) and Sm(III). K E Y W O R D S biosorption, Botryosphaeria rhodina MAMB-05, Langmuir and Freundlich models, rare-earth elements

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Section: Resultsmentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biosorption of lanthanum and samarium by viable and autoclaved mycelium of Botryosphaeria rhodina MAMB‐05

Giese

Dekker

Barbosa-Dekker

2019

Biotechnology Progress

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“…15 Botryosphaeran is an exocellular (1 → 3)(1 → 6)-β-D-glucan produced by the ascomyceteous, filamentous, endophytic, fungus Botryosphaeria rhodina isolate MAMB-05 approximately 22% branching first described in 2003. 19 An overview of botryosphaeran's biological activities was recently reviewed by Dekker et al 20 and Dekker and Barbosa-Dekker. 21 Botryosphaeran does not exert mutagenic and genotoxic effects, [22][23][24][25] and is classified in the category generally recognized as safe of the US Food and Drug Administration.…”

Section: Introductionmentioning

confidence: 99%

Oral administration of botryosphaeran [(1 → 3)(1 → 6)‐β‐d‐glucan] reduces inflammation through modulation of leukocytes and has limited effect on inflammatory nociception

Silva

Pereira

Santos

et al. 2022

Cell Biochemistry & Function

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Several biological activities of the fungal exopolysaccharide (1 → 3)(1 → 6) -β-D-glucan (botryosphaeran) have been described in the literature, but its effects on inflammation have not been evaluated. This study aimed to investigate the action of botryosphaeran on experimental mice models of carrageenan-induced acute pleurisy and acute paw edema, and complete Freund's adjuvant-induced persistent paw edema. All botryosphaeran doses tested (1.0, 2.5, 5.0, and 10.0 mg/kg birth weight [b.w.], orally administered) reduced leukocyte recruitment, nitric oxide (NO) levels, and protein extravasation in the pleural cavity. Botryosphaeran (5 mg/kg b.w.) did not diminish edema and mechanical hyperalgesia in the paw within 4 h; however, cold allodynia was alleviated within the first 2 h. In the persistent paw inflammation model, the effects of daily oral administration of botryosphaeran (5 mg/kg b.w.) were evaluated over 3 and 7 days. The fungal β-glucan significantly reduced the levels of the cytokines, tumor necrosis factor(TNF)-α, interleukin (IL)-6), and IL-10, in the paw homogenates in both protocols, while paw edema and the levels of advanced oxidation protein products (AOPP) only diminished on Day 7. No effect in mechanical hyperalgesia was observed. Oral treatment for 3 or 7 days also decreased the plasma levels of NO, AOPP, TNF-α, and IL-10. On Day 7, the number of leukocytes in the blood was also reduced by this treatment. Importantly, botryosphaeran did not induce inflammation in mice when administered alone over 7 days. This study demonstrated the anti-inflammatory and antinociceptive potential of botryosphaeran in these experimental models, making this fungal β-glucan a new possibility for complementary treating acute and chronic inflammation.

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“…12 This EPS consists of a main chain of D-glucose residues linked by β-(1→3) glucosidic bonds, containing approximately 22% side-branch residues at carbon 6, comprising glucose and gentiobiose, 12 and exists in a triple helix conformation. 13 Several studies have demonstrated the biological functions of Botryosphaeran, which have been summarized by Dekker et al 14 Among these functions are anticlastogenic activity, 15 antiproliferative and proapoptotic activity against breast cancer MCF-7 cells (in vitro), 16 hypoglycemic and hypolipidemic activities in diabetic and hyperlipidemic Wistar rats, respectively, 17 anti-obesogenic effect, 10 antioxidant, 18 and antitumor activities in obese and nonobese rats bearing Walker-256 tumor. [19][20][21] These effects were observed in rats and mice on a 15-day period of treatment with Botryosphaeran.…”

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confidence: 99%

Extended treatment with (1→3)(1→6)‐β‐d‐glucan (Botryosphaeran) reduces obesity and its comorbidities in high‐fat/high‐sugar diet‐fed rats

Ribeiro

Silva

Giordani

et al. 2022

Cell Biochemistry & Function

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Obesity is associated with other diseases such as diabetes and cancer. Botryosphaeran, a fungal (1→3)(1→6)‐β‐d‐glucan, is described to present antimutagenic, hypoglycemic, hypocholesterolemic, and antitumor activities when administered by gavage over 15 days in rats and mice. Thus, the present study aims to analyze the metabolic effects of Botryosphaeran (12 mg/kg body weight/day) treatment over 30 days in obese Wistar male rats. Obesity was induced in the rats by a high‐fat/high‐sugar diet for 8 weeks. Control rats received a standard diet. On the 5th week, Botryosphaeran treatment commenced. Groups: control, obese, and obese+Botryosphaeran 30 days. In the 8th week, obesity was characterized. Feed intake, glucose and lipid profiles, glucose tolerance, and insulin sensitivity were analyzed. Obese rats showed accumulation of visceral adipose tissue, reduction of muscle mass, glucose intolerance, insulin resistance, hyperglycemia, and dyslipidemia. Botryosphaeran effectively reduced weight gains and the accumulation of retroperitoneal adipose tissue, corrected the levels of glucose, triglycerides, and very low‐density lipoprotein‐cholestrol, and improved insulin sensitivity. Treatment for 30 days was effective in maintaining the beneficial effects demonstrated by this β‐glucan when administered for 15 days without promoting side effects. Treatment with (1→3)(1→6)‐β‐ d‐glucan presented anti‐obesogenic and beneficial metabolic effects in Wistar rats; important for the treatment of obesity and its comorbidities.

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Botryosphaeran – A Fungal Exopolysaccharide of the (1→3)(1→6)-β-D-Glucan Kind: Structure and Biological Functions

Cited by 17 publications

References 169 publications

Biosorption of lanthanum and samarium by viable and autoclaved mycelium of Botryosphaeria rhodina MAMB‐05

Biosorption of lanthanum and samarium by viable and autoclaved mycelium of Botryosphaeria rhodina MAMB‐05

Oral administration of botryosphaeran [(1 → 3)(1 → 6)‐β‐d‐glucan] reduces inflammation through modulation of leukocytes and has limited effect on inflammatory nociception

Extended treatment with (1→3)(1→6)‐β‐d‐glucan (Botryosphaeran) reduces obesity and its comorbidities in high‐fat/high‐sugar diet‐fed rats

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