Botrytis-Biology, Detection and Quantification

Dewey, F. M.; Grant-Downton, Robert

doi:10.1007/978-3-319-23371-0_2

Cited by 11 publications

(10 citation statements)

References 73 publications

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“…B. cinerea has been classified as the second most important plant pathogen and damages more than 200 plant species, especially strawberry, tomato, cucumber, potato, crops, and ornamental plants. , Besides, B. cinerea causes serious storage disease of fruits and vegetables and significant economic losses each year .…”

Section: Introductionmentioning

confidence: 99%

“…1 B. cinerea has been classified as the second most important plant pathogen and damages more than 200 plant species, especially strawberry, tomato, cucumber, potato, crops, and ornamental plants. 2,3 Besides, B. cinerea causes serious storage disease of fruits and vegetables and significant economic losses each year. 4 The reason why it can cause serious diseases is because this pathogen displays a capacity to kill host cells through the production of toxins, reactive oxygen species (ROS), degrading enzymes, and the induction of a plant-produced oxidative burst.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Also, B. cinerea has the ability to survive saprophytically on dead hosts and also overcome environmental stress through clamydospore, dormant mycelia, and sclerotia. , A variety of compounds are used for Botrytis control, such as boscalid, penthiopyrad, fluopyram, adepidyn, pyrimethanil, fludioxonil, and fenhexamid, but most of them are synthetic fungicides . The contamination of fruits, vegetables, or crops with residues from synthetic fungicides and its effect on human health or environment, as well as the problem of increased resistance caused by long-term use of fungicides that have been developed are the main challenges. , Biofungicides, biochemical pesticides, and microbial pesticides have been and will continue to be the focus of research in order to develop new, efficient, and low-toxic biological control agents against B.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of a Natural Hybrid Polyketide Produced by Endophytic Cladosporium sphaerospermum for Biocontrol of Phytopathogenic Fungus Botrytis cinerea

Pan

Yang

Zhu

et al. 2023

J. Agric. Food Chem.

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The endophytic fungus Cladosporium sphaerospermum WBS017 exhibits broad-spectrum activity against plant pathogens, with particular effectiveness against Botrytis cinerea. Subsequently, a compound is isolated from strain WBS017 as the main active ingredient against B. cinerea using activity-guided separation and identified as hybrid polyketide (namely cladodionen, CLD) using UV, MS, NMR, etc. In vitro and in vivo antifungal activity tests demonstrate that CLD effectively inhibits the mycelial growth and spore germination, with an IC 50 value of 1.13 and 0.095 mM, respectively, and exerts antifungal and fresh-keeping effects on both strawberry and tomato. Microscopy analysis reveals that the inhibitory effects of CLD on hyphae and spore germination are attributed to a decrease in structural stability of mycelia cells as well as the accumulation of reactive oxygen species (ROS). Furthermore, transcriptome analysis further indicates that spore germination is inhibited by suppressing the transcription levels of membrane or membrane-related genes, disturbing the balance of ROS metabolism, altering the primary metabolic pathways, genetic information processing, and cellular processes. Importantly, CLD demonstrates no significant toxicity on zebrafish embryos even at a concentration of 0.226 mM, indicating its potential as a safe biological-control agent. In summary, CLD would be a novel potential biological-control agent and can be considered as a promising fungicide to control B. cinerea.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Discovery of a Natural Hybrid Polyketide Produced by Endophytic Cladosporium sphaerospermum for Biocontrol of Phytopathogenic Fungus Botrytis cinerea

Pan

Yang

Zhu

et al. 2023

J. Agric. Food Chem.

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“…are easily recognized followed by the gray and/or brown conidial structures on the surface of infected host (Khazaeli et al, 2010). However, the initial or dormant and endophytic stages of infections are difficult to detect in non-symptomatic material (Molly and Grant-Downton, 2015). Also, the morphologies of conidiospores of Botrytis ssp.…”

Section: Case Study: Detection Of Botrytis Spp Of Temperate Grain Lementioning

confidence: 99%

“…is now somewhat routine 2 . The laboratory-based plate-trapped antigen ELISA (Meyer et al, 2000)was time-consuming, involving an overnight antigen coating step with extracts from infected plant tissues followed by sequential incubations with a monoclonal antibody, a secondary enzyme conjugated anti-mouse antibody and a substrate with washings between each step (Molly and Grant-Downton, 2015). By increasing the concentration of the secondary reporter antibody, the total time involved for diagnosis was subsequently reduced to 20 min (Dewey et al, 2000).…”

Section: Case Study: Detection Of Botrytis Spp Of Temperate Grain Lementioning

confidence: 99%

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

2019

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Plant pathogens reduce global crop productivity by up to 40% per annum, causing enormous economic loss and potential environmental effects from chemical management practices. Thus, early diagnosis and quantitation of the causal pathogen species for accurate and timely disease control is crucial. Botrytis Gray Mold (BGM), caused by Botrytis cinerea and B. fabae , can seriously impact production of temperate grain legumes separately or within a complex. Accordingly, several immunogenic and molecular probe-type protocols have been developed for their diagnosis, but these have varying levels of species-specificity, sensitivity and consequent usefulness within the paddock. To substantially improve speed, accuracy and sensitivity, advanced nanoparticle-based biosensor approaches have been developed. These novel methods have made enormous impact toward disease diagnosis in the medical sciences and offer potential for transformational change within the field of plant pathology and disease management, with early and accurate diagnosis at the point-of-care in the field. Here we review several recently developed diagnostic tools that build on traditional approaches and are available for pathogen diagnosis, specifically for Botrytis spp. diagnostic applications. We then identify the specific gaps in knowledge and current limitations to these existing tools.

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Genomic Prediction of Strawberry Resistance to Postharvest Fruit Decay Caused by the Fungal PathogenBotrytis cinerea

Petrasch

Mesquida-Pesci

Pincot

et al. 2021

Preprint

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Gray mold, a disease of strawberry (Fragaria × ananassa) caused by the ubiquitous necrotroph Botrytis cinerea, renders fruit unmarketable and causes economic losses in the postharvest supply chain. To explore the feasibility of selecting for increased resistance to gray mold, we undertook genetic and genomic prediction studies in strawberry populations segregating for fruit quality and shelf life traits hypothesized to pleiotropically affect susceptibility. As predicted, resistance to gray mold was heritable but quantitative genetically complex. While every individual was susceptible, the speed of symptom progression and severity differed. Narrow-sense heritability ranged from 0.38-0.71 for lesion diameter (LD) and 0.39-0.44 for speed of emergence of external mycelium (EM). Even though significant additive genetic variation was genome wide observed for LD and EM, the phenotypic ranges were comparatively narrow and genome-wide analyses did not identify any large effect loci. Genomic selection accuracy ranged from 0.28-0.59 for LD and 0.37-0.47 for EM. Additive genetic correlations between fruit quality and gray mold resistance traits were consistent with prevailing hypotheses: LD decreased as titratable acidity increased, whereas EM increased as soluble solid whole genome content decreased and firmness increased. We concluded that phenotypic and genomic selection could be regression effective for reducing LD and increasing EM, especially in long shelf life populations, but that a significant fraction of the genetic variation for resistance to gray mold was caused by the pleiotropic effects of fruit quality traits that differ among market and shelf life classes.

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Botrytis-Biology, Detection and Quantification

Cited by 11 publications

References 73 publications

Discovery of a Natural Hybrid Polyketide Produced by Endophytic Cladosporium sphaerospermum for Biocontrol of Phytopathogenic Fungus Botrytis cinerea

Discovery of a Natural Hybrid Polyketide Produced by Endophytic Cladosporium sphaerospermum for Biocontrol of Phytopathogenic Fungus Botrytis cinerea

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

Genomic Prediction of Strawberry Resistance to Postharvest Fruit Decay Caused by the Fungal PathogenBotrytis cinerea

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