Botulinum toxin type A on cutaneous flap viability in diabetic and tobacco-exposed rats

Camargo, Cristina Pires; Jácomo, Alfredo Luiz; Battlehner, Cláudia Naves; Lemos, Miriam; Saldiva, Paulo Hilário Nascimento; Martins, Mílton A.; Munhoz, Alexandre Mendonça; Gemperli, Rolf

doi:10.1590/s0102-865020150090000009

Cited by 18 publications

(16 citation statements)

References 27 publications

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“…Moreover, there was no difference in terms of flap viability between health (no exposure) and smoke-exposure animals in this previous study 9 . We hypothesized that the 3x10cm flap would cause a very severe vascular insufficiency resulting in necrosis, and that no additional deleterious environmental factor (tobacco-exposure) would increase necrosis.…”

Section: Resultscontrasting

confidence: 52%

“…Although BontA showed a difference in health and diabetic animals, there was no BontA effect in the flap viability for the tobacco-exposed group 9 . This hypothesis was based on other studies 10,11 .…”

Section: Introductionmentioning

confidence: 76%

“…For this reason, several strategies were studied to decrease surgical complications [4][5][6] , one of the strategies was to use BontA as a vasodilator [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

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The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats

Camargo

Fernandes²,

Lee³

et al. 2016

Acta Cir. Bras.

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PURPOSE:To evaluate the effect of Botulinum Toxin A in different time of tobacco exposure.METHODS: 60 male, Wistar rats were divided into two tobacco exposure groups: a 2-month or a 4-month regimen. After this period, these two groups were subdivided as two: saline solution(SS) or botulinum toxin A(Bonta), at the time of the surgery. Seven days before the SS or Bonta injection, the animals were submitted to a random flap (3x10cm). On the seventh postoperative day, all animals were assessed for total flap area, viable area, and the viable/ total area ratio. RESULTS:This study showed a difference between groups 2-month saline vs. BontA injection (p=0.04); groups 4-month saline vs.BontA injection (p=0.001); groups 2-month saline vs. 4-month BontA (p=0.003), and, between groups 2-month BontA vs. 4-month saline(p=0.03). CONCLUSIONS:Botulinum Toxin A increased random flap viability in tobacco-exposed rats. Two months of tobacco exposure had the same effect as exposure for four months.

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Section: Resultscontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 76%

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The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats

Camargo

Fernandes²,

Lee³

et al. 2016

Acta Cir. Bras.

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“…We adopted this model to mimic a moderate smoker patient. According to our previous study this model induced a carboxyhemoglobin level similar to a moderate smoker 17 , 18 . Moreover, we exposed the animal for two-months to cause a tobacco endothelial dysfunction 11 , 17 .…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic acid in tobacco-exposed rats. Inflammatory reaction, and duration of effect

Camargo

Frassei²,

Silva³

et al. 2019

Acta Cir. Bras.

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Purpose To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats. Methods Ten Wistar rats were divided into two groups: tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks. After this period, all the animals were euthanized, and a specimen of was collected to evaluate inflammatory cells, fibroblasts, and fibrosis by HE. Results This study showed a higher inflammatory reaction in TEG than CG: inflammatory cell-count (CG: 1.07±0.9; TEG: 8.61±0.36, p<0.001); fibroblast count (CG: 2.92±0.17; TEG: 19.14±0.62, p<0.001), and fibrosis quantification (CG: 2.0; TEG: 3.75, p<0.001). The analysis of the HA volume in nine weeks in the dorsal region did not show a difference between groups (p=0.39). Conclusions This study suggested that the HA injection in the TEG caused an increase in inflammatory cell count, fibroblast, and fibrosis quantification when compared to the CG. There was no difference in the duration of effect of HA between the groups.

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“…In one study, BoNT/A was used to prevent unfavourable effects of cigarette smoke in Wistar albino rats . In another study, it increased skin‐flap viability in diabetic rats, which was associated with an increase of lumen diameter, external arterial diameter and lumen/wall thickness ratio . At the molecular level, BoNT/A affected the expression of vascular endothelial growth factor, platelet endothelial cell adhesion molecule 1, CD31, CD34, interleukin (IL)‐1 and tumour necrosis factor‐α in cutaneous flaps .…”

Section: Experimental Studies Of Cutaneous Effects Of Botulinum Neuromentioning

confidence: 99%

The non-neuronal and nonmuscular effects of botulinum toxin: an opportunity for a deadly molecule to treat disease in the skin and beyond

Grando

Zachary

2018

Br J Dermatol

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There is growing evidence that botulinum neurotoxins (BoNTs) exhibit biological effects on various human cell types with a host of associated clinical implications. This review aims to provide an update on the non-neuronal and nonmuscular effects of botulinum toxin. We critically analysed recent reports on the structure and function of cellular signalling systems subserving biological effects of BoNTs. The BoNT receptors and intracellular targets are not unique for neurotransmission. They have been found in both neuronal and non-neuronal cells, but there are differences in how BoNT binds to, and acts on, neuronal vs. non-neuronal cells. The non-neuronal cells that express one or more BoNT/A-binding proteins, and/or cleavage target synaptosomal-associated protein 25, include: epidermal keratinocytes; mesenchymal stem cells from subcutaneous adipose; nasal mucosal cells; urothelial cells; intestinal, prostate and alveolar epithelial cells; breast cell lines; neutrophils; and macrophages. Serotype BoNT/A can also elicit specific biological effects in dermal fibroblasts, sebocytes and vascular endothelial cells. Nontraditional applications of BoNT have been reported for the treatment of the following dermatological conditions: hyperhidrosis, Hailey-Hailey disease, Darier disease, inversed psoriasis, aquagenic palmoplantar keratoderma, pachyonychia congenita, multiple eccrine hydrocystomas, eccrine angiomatous hamartoma, eccrine sweat gland naevi, congenital eccrine naevus, Raynaud phenomenon and cutaneous leiomyomas. Experimental studies have demonstrated the ability of BoNT/A to protect skin flaps, facilitate wound healing, decrease thickness of hypertrophic scars, produce an anti-ageing effect, improve a mouse model of psoriasiform dermatitis, and have also revealed extracutaneous effects of BoNT arising from its anti-inflammatory and anticancer properties. BoNTs have a much wider range of applications than originally understood, and the individual cellular responses to the cholinergic impacts of BoNTs could provide fertile ground for future studies.

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Botulinum toxin type A on cutaneous flap viability in diabetic and tobacco-exposed rats

Cited by 18 publications

References 27 publications

The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats

The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats

Hyaluronic acid in tobacco-exposed rats. Inflammatory reaction, and duration of effect

The non-neuronal and nonmuscular effects of botulinum toxin: an opportunity for a deadly molecule to treat disease in the skin and beyond

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