Bovine bone implant with bovine bone morphogenetic protein in healing a canine ulnar defect

Abstract

Xenograft is considered an alternative material for bone transplantation, but its bone healing capacity is inferior compared to that of autografts and allografts. Here, we tested whether bone morphogenetic protein (BMP) addition enhances the suitability of demineralized xenogeneic bovine bone for bone grafting in dogs, and whether xenogeneic bone is a suitable carrier material for BMPs. The capacity of demineralized bovine bone implants, with and without native partially purified bovine BMP, to heal a 2-cm ulnar defect was determined in six dogs over a follow-up time of 20 weeks. No instances of bone union were seen, but there was slightly more bone formation in the xenografts with BMP, though the difference was not statistically significant. The ulnas treated with an implant with BMP were also mechanically stronger, but the difference was not significant. Computed tomography scans showed no differences in the implant area in bone density, bone mineral content, or bone cross-sectional area. It is concluded that native, partially purified BMP does not sufficiently improve the suitability of bovine demineralized xenografts as a bone substitute material for dog. Demineralized xenogeneic bone does not seem to be a feasible carrier material for BMP.

Influence of ethylene oxide sterilization on the activity of native reindeer bone morphogenetic protein

“…The ability of BMPs to induce new bone and to heal bone defects has been well established in many experimental animal and clinical studies [7,8,9,11,12,13,14,21,22,24]. The sterilization of BMP before preclinical and clinical application is mandatory but problematic because BMPs do not tolerate heat management.…”

The efficacy of biodegradable mesh as a fixation device for support of autogenous onlay bone grafts: a radiographic and histomorphometric analysis

“…Their bone generation potential is considered to be 9 inferior when compared to autografts (Tuominen et al 2001 (Stavropoulos et al 2001(Stavropoulos et al , 2003. In addition, xenografts do however have the same advantage as alloplastic bone grafts, being available in an unlimited supply.…”

Bovine Bone Matrix Action Associated With Morphogenetic Protein in Bone Defects in Rats Submitted to Alcoholism

“…After lyophilization, BMPs are linked to a carrier, which has the purpose of preserving the surgical cavity for a longer period, as it would normally be rapidly resorbed by the organism. Therefore, one characteristic of these morphogenetic proteins is the need of a vehicle, which can be the demineralized bovine matrix (Damien et al, 1995;Tuominen et al, 2001;Ueda et al, 2001;Guimarães et al, 2004;Curi et al, 2008;Ho et al, 2010).…”