Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus

Barry, Aline Fernandes; Alfieri, Alice Fernandes; Stipp, Danilo Tancler; Alfieri, Amauri Alcindo

doi:10.1590/s1516-89132009000700006

Cited by 18 publications

(27 citation statements)

References 13 publications

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“…Similar outbreaks have been reported in cattle Barry et al, 2009), swine (Cappellaro, 1998), equine ( Cappellaro et al, 1988), dog (Hagiwara et al, 1989;Dezengrini et al, 2007;Decaro et al, 2010), sheep (Pass et al, 1982), feline (Pedersen et al, 2008) and avian (Circella et al, 2007) and among wild animals of zootecnical breeding, such as collared peccary (Catroxo et al, 1995), rhea (Catroxo et al, 1996 a); white lipped peccary, (Catroxo et al, 1996 b), ostrich (Els & Josling, 1998) and wild boar (Bersano et al, 2001).…”

Section: Discussionsupporting

confidence: 84%

Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil

Catroxo

Miranda

Lavorenti³

et al. 2010

Int. J. Morphol.

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SUMMARY:Coronaviruses are enveloped, positive-stranded RNA, belongs to the Nidovirales order, Coronaviridae family and are classified into three groups. Groups 3a and 3c comprising the avian coronaviruses. They are worldwide in distribution and can cause respiratory, enteric and in some cases hepatitis and neurological diseases in a wide variety of animals, resulting in economic losses to breeders and breeding. In this study, we describe an outbreak of diarrhea in a herd of capybaras found in the city of Piracicaba, São Paulo, Brazil. The animals had sudden symptoms of anorexia, profuse and watery diarrhea, yellowish or greenish, dehydration, weight loss, sharp and twisting of limbs leading to death in less than a week, mostly weaned and with an age of three months. A total of 59 stool samples and 10 fragments of small intestine were processed for negative staining technique (rapid preparation). By transmission electron microscope a large number of particles with morphology similar to coronavirus, pleomorphic, rounded or elongated features with radial projections form a corona and measuring 80-140 nm in diameter, was visualized in all samples analyzed. The technique was extremely useful for rapid viral diagnosis in the affected animals.

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Section: Discussionsupporting

confidence: 84%

Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil

Catroxo

Miranda

Lavorenti³

et al. 2010

Int. J. Morphol.

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“…With this information, we evaluated the probability that three known enteric RNA viruses (RVA, BCoV, and aichivirus B) were involved in the etiology of this severe diarrhea outbreak. Although BCoV is considered an important etiological agent of neonatal diarrhea worldwide [4,10,13], it had no influence on this outbreak in dairy calves, because all diarrheic fecal samples included in the analysis were negative for this virus.…”

Section: Discussionmentioning

confidence: 99%

“…In dairy cattle herds, neonatal diarrhea is considered one of the main infectious diseases in suckling calves worldwide [1,4]. Some classes of microorganisms, such as bacteria, protozoa, and viruses, are infectious agents and are considered determinants of neonatal diarrhea [9].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of VP1 and RdRP genes of Brazilian aichivirus B strains involved in a diarrhea outbreak in dairy calves

et al. 2017

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Aichivirus B has been reported worldwide in calves and adult cattle with and without diarrhea. The aim of this study was to describe the molecular characteristics of the RdRP and VP1 genes of aichivirus B strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production Brazilian dairy cattle herd. Preliminary laboratory analysis ruled out important enteropathogens (Cryptosporidium spp; Eimeria spp., E. coli F5, and bovine coronavirus). Fecal samples from diarrheic (n = 24) and asymptomatic (n = 5) calves up to 30 days old were collected for virological analysis. RT-PCR assays were performed for the detection of aichivirus B RdRP and VP1 genes and for rotavirus A VP7 and VP4 genes in fecal samples. Asymptomatic calves (control group) were negative for both viruses. Aichivirus B and rotavirus A G10P[11] genotypes were found in 54.2% (13/24) and 25% (6/24) of the diarrheic fecal samples, respectively. Aichivirus B was only identified (83.3%, 10/12) in calves up to two weeks old. Phylogenetic analysis based on the RdRP gene grouped the Brazilian strains in a new branch within the aichivirus B group. Comparative analysis of the nucleotide sequence of the VP1 gene of Brazilian and Chinese aichivirus B strains allowed the strains identified in this study to be classified in the putative lineage 1. This is the first description of a high rate of aichivirus B detection in a diarrhea outbreak in dairy calves, and the first phylogenetic study of the VP1 gene of aichivirus B wild-type strains performed in South America.

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“…The coronavirus infect both small intestine and large intestine to cause severe disease [10]. In calves, group A rotavirus and coronavirus, either single or in combination, are predominately associated with neonatal (mostly up to 5-15 day old) diarrhoea [11] [12]. The diagnosis of diarrhoea (enteritis) cases is cumbersome due to non specific nature of clinical signs/lesions, interaction of polymicrobial agents and involvement of intrinsic and extrinsic risk factors [13].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and molecular detection of natural cases of bovine rotavirus and coronavirus infection causing enteritis in dairy calves

Singh

et al. 2020

Microbial Pathogenesis

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A B S T R A C TBovine rotavirus (BRoV) and bovine coronavirus (BCoV) are major enteric viral pathogens responsible for calve diarrhoea. They are widespread both in dairy and beef cattle throughout the world and causing huge economic losses. The diagnosis of these agents is very difficult due to non-specific nature of lesions and the involvement of some intrinsic and extrinsic risk factors. We performed postmortem of 45 calves, which was below three months of age. Out of 45 necropscid calves, three (6.66%) cases were positive for BRoV and four (8.88%) cases were found positive for BCoV, screened by reverse transcriptase polymerase chain reaction (RT-PCR). Further RT-PCR positive cases were confirmed by immunohistochemistry (IHC) in paraffin-embedded intestinal tissue sections. Three cases of enteritis caused by BRoV showed the hallmark lesions of the shortening and fusion of villi, denudation and infiltration of mononuclear cells in the lamina propria. The BRoV antigen distribution was prominent within the lining epithelium of the villi, peyer's patches in the ileum and strong immunoreactions in the lymphocytes and some macrophages of the mesenteric lymph nodes. Four cases in which BCoV was detected, grossly lesions characterized by colonic mucosa covered with thick, fibrinous and diphtheritic membrane. Histopathologically, jejunum showed skipping lesion of micro-abscesses in crypts. The BCoV antigen distribution was prominent within the necrotic crypts in the jejunum and cryptic micro-abscesses in the colon and ileum. It is the first report of BRoV and BCoV antigen demonstration in the jejunum, colon, ileum, Peyer's patches and mesenteric lymph nodes of naturally infected calves from India by using IHC.

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Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus

Cited by 18 publications

References 13 publications

Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil

Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil

Phylogenetic analysis of VP1 and RdRP genes of Brazilian aichivirus B strains involved in a diarrhea outbreak in dairy calves

Immunohistochemical and molecular detection of natural cases of bovine rotavirus and coronavirus infection causing enteritis in dairy calves

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