Bovine endothelial cells transformed in vitro by benzo(a)pyrene

Abstract

A cloned strain of bovine vascular endothelial cells with a finite in vitro lifespan was treated with benzo(a)pyrene (BP) after approximately 75% of its lifespan was completed. Untreated cultures of this strain senesced upon serial subcultivation and contained large, nondividing cells. In three out of seven trials, BP treatment produced transformed lines with altered phenotypic characteristics. The transformed cells appeared in the cultures concomitant with the senescence of the parent cells. All transformed cell lines examined exhibited indefinite lifespans and altered karyotypes. Two of the lines retained most of the characteristics of normal endothelial cells, except that one became aneuploid and the other polyploid. Neither of these lines formed tumors when inoculated into nude mice. The remaining two lines retained mostly diploid karyotypes, but a high percentage of cells contained Robertsonian translocations. In one line cell volume was markedly reduced. In addition, these lines grew in multilayers, were anchorage independent, and proliferated in medium containing 0.5% serum. When 10(7) cells of these lines were injected into nude mice, tumors appeared within 1 week and were identified as malignant hemangioendotheliomas of bovine origin.

Paracrine and autocrine effects of fibroblast growth factor-4 in endothelial cells

“…Then, radioactivity bound to high a nity FGFRs was measured FGF2 and FGF4 are able to exert a signi®cant mitogenic response also in this endothelial cell type (data not shown). Similar results were obtained when conditioned media and recombinant proteins were tested in the fetal bovine aortic endothelial GM7373 cell line (Grinspan et al, 1983) (data not shown).…”

Plasma sodium stiffens vascular endothelium and reduces nitric oxide release

“…GM 7373 cells were used because basal nitric oxide metabolism is pronounced compared with EA.hy 926 cells and thus more suitable for nitrite analysis. In short, confluent GM 7373 cells (45) were cultured in T 75 flasks in low sodium (135 mM sodium, in the absence or presence of 0.45 nM aldosterone) and high sodium (150 mM, in the absence or presence of aldosterone) medium. Mannitol (30 mM) was added to the low sodium medium for maintaining isoosmolality.…”

BSA conjugates bearing multiple copies of the basic domain of HIV-1 Tat: Prototype for the development of multitarget inhibitors of extracellular Tat

“…HL3T1 cells were maintained in DMEM (Gibco, Grand Island, NY) supplemented with 10% FCS, 100 U/ml penicillin and 100 U/ml streptomycin (Broder et al, 1997). Transformed fetal bovine aortic endothelial GM7373 cells (Grinspan et al, 1983) were grown in DMEM supplemented with 10% FCS.…”