Bovine humoral immune response to Cryptosporidium parvum

Mosier, Derek A.; Kuhls, Thomas L.; Simons, K R; Oberst, Richard D.

doi:10.1128/jcm.30.12.3277-3279.1992

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…Infected hosts can be identified by an enzyme-linked immunosorbent assay (ELISA) that detects serum antibody to crude oocyst extracts. [5][6][7][8][9][10] ELISA methodologies can include antibody isotype determination through the use of immunoglobulin isotype-specific reagents. 5,[9][10][11] ELISA is often accompanied by Western blots that use crude extracts to determine the molecular sizes of proteins to which the antibodies bind.…”

Section: Introductionmentioning

confidence: 99%

“…5,9‐11 ELISA is often accompanied by Western blots that use crude extracts to determine the molecular sizes of proteins to which the antibodies bind. 6‐8,10 Recently, the ELISA format was modified to use recombinant and partially purified sporozoite proteins to characterize serum antibody responses. 12 These assays provide information about C. parvum infection rates.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Mucosally Delivered Antibody to Cryptosporidium parvum p23 in Infected Calves

Wyatt

Perryman

2000

Annals of the New York Academy of Sciences

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We have developed an assay to detect mucosally delivered antibody to Cryptosporidium parvum sporozoite antigens. We absorbed a recombinant 23‐kD sporozoite protein to polystyrene microspheres, and used flow cytometry to detect, titer, and determine the isotype of antibody to p23 that was shed in the feces of experimentally infected calves. Noninoculated calves have low levels of mucosal antibody to p23, with IgG1 as the predominant isotype. Antibody titers rise in inoculated calves as the animals recover from cryptosporidiosis. A calf that was naturally protected from cryptosporidiosis had mucosal IgM and IgG1 isotype anti‐p23 antibodies prior to challenge with C. parvum oocysts. Ten days after challenge, the calf had high titers of IgM, IgA, IgG1, and IgG2 anti‐p23 antibodies. Together, the data show that this method can be used to assess mucosally delivered antibody to C. parvum.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Mucosally Delivered Antibody to Cryptosporidium parvum p23 in Infected Calves

Wyatt

Perryman

2000

Annals of the New York Academy of Sciences

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“…Antibody levels then rose over the next 3-4 weeks. By 3 weeks after infection, serum IgG isotype antibody reacted in Western blots to C. parvum antigens of 11-14, 38, 55 and 90-140 kDa (Mosier et al, 1992). Collectively, the data suggest that, after infection, calves develop an immune response that is detectable in the serum.…”

Section: Time Course Of Serum and Mucosally Delivered Antibody Responmentioning

confidence: 77%

Cryptosporidium parvumand mucosal immunity in neonatal cattle

Wyatt

2000

Anim. Health. Res. Rev.

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Cryptosporidium parvumis an important zoonotic protozoan pathogen that causes acute infection and self-limiting gastrointestinal disease in neonatal calves. There are currently no consistently effective antimicrobials available to control cryptosporidiosis. Therefore, immunotherapeutic and vaccination protocols offer the greatest potential for long-term control of the disease. In order to devise effective control measures, it is important to better define mucosal immunity toC. parvumin young calves. This review summarizes the information that has accumulated over the last decade which helps to define the intestinal mucosal immune system in neonatal calves, and the events that occur in the intestinal mucosa after infection byC. parvum.

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“…Previous studies using bovine and human sera have shown a lack of correlation between the detection of anti-cryptosporidial IgG by ELISA and that by immunoblotting (16,29). Since C. parvum may have multiple antigenic carbohydrates, glycoproteins, and glycolipids, many of these antigens may be detected in ELISAs but may not be recognized in denatured samples used for immunoblots.…”

Section: Discussionmentioning

confidence: 99%

“…The immunoblot technique to detect anti-cryptosporidial antibodies was adapted from a method described previously (16). Briefly, 10 6 sonicated cryptosporidial oocysts were denatured and reduced by boiling in 1% sodium dodecyl sulfate (SDS) and 2.5% 2-mercaptoethanol.…”

Section: Methodsmentioning

confidence: 99%

Enteral human serum immunoglobulin treatment of cryptosporidiosis in mice with severe combined immunodeficiency

et al. 1995

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The anti-cryptosporidial immunoglobulin G antibodies in two commercially available human serum immunoglobulin (HSIG) products were quantified and characterized. The mean level of Cryptosporidium parvum-specific immunoglobulin G in HSIG was eightfold higher than the antibody level found in the sera of three immunocompetent individuals convalescing from cryptosporidiosis. However, HSIG products displayed no reactivity to cryptosporidial antigens in immunoblot analyses, while convalescent-phase sera demonstrated characteristic banding patterns. When HSIG was given to newborn severe combined immunodeficiency (scid) mice before and shortly after experimental infection, a decreased intensity of infection was observed in the intestines of the mice compared with that of control mice. However, there was no difference in mortality or histopathologic findings in the intestines of HSIG-treated and control mice when treatment was not started until 22 days of age. These results indicate that HSIG may be beneficial when given prophylactically; however, HSIG cannot eradicate cryptosporidia from mucosal surfaces in an established infection.

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Bovine humoral immune response to Cryptosporidium parvum

Cited by 13 publications

References 13 publications

Detection of Mucosally Delivered Antibody to Cryptosporidium parvum p23 in Infected Calves

Detection of Mucosally Delivered Antibody to Cryptosporidium parvum p23 in Infected Calves

Cryptosporidium parvumand mucosal immunity in neonatal cattle

Enteral human serum immunoglobulin treatment of cryptosporidiosis in mice with severe combined immunodeficiency

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