Bovine intramammary infection associated immunogenic surface proteins of Streptococcus uberis

Dego, Oudessa Kerro; Almeida, Ricardo Augusto Monteiro de Barros; Saxton, Arnold M.; Abdi, Reta D.; Ensermu, Desta Beyene; Oliver, S. P.

doi:10.1016/j.micpath.2017.12.046

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…This leads us to conclude that, in pbMEC, we were able to elicit a differential immune response at the mRNA level by stimulating isolated cells. This is in line with findings reported in the literature (Bauer et al, 2015;Kerro Dego et al, 2018;Ying et al, 2021). Moreover, the PCBUS showed similar or higher gene expression profiles compared with pbMEC; thus, PCBUS can be used for bovine mastitis studies.…”

Section: Discussionsupporting

confidence: 93%

Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices

Filor

Seeger

Buhr

et al. 2022

Journal of Dairy Science

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Mastitis in cattle is a major health problem as well as incurring high costs for the dairy industry. To assess the suitability of precision-cut bovine udder slices (PCBUS) for bovine mastitis studies, we infected PCBUS with 2 different Staphylococcus aureus strains. Accordingly, we investigated both the tissue response to infection based on immune mediators at the mRNA and protein levels and the invasion of bacteria within the tissue. The studied proteins represent immune mediators of early inflammation [IL-1β, tumor necrosis factor-α (TNF-α), prostaglandin E 2 (PGE 2 )] and showed a time-dependent increase in concentration. Infection of PCBUS with S. aureus resulted in increased expression of proinflammatory cytokines and chemokines such as TNF-α, C-C motif chemokine ligand 20 (CCL20), IL-1β, IL-6, and IL-10, but not C-X-C motif chemokine ligand 8 (CXCL8), lingual antimicrobial peptide (LAP), or S100 calcium binding protein A9 (S100A9) at the mRNA level. To compare the data acquired with this model, we carried out investigations on primary bovine mammary epithelial cells. Our results showed that the immune responses of both models-PCBUS and primary bovine mammary epithelial cells-were similar. In addition, investigations using PCBUS enabled us to demonstrate adherence of bacteria in the physiological cell network. These findings support the use of PCBUS in studies designed to further understand the complex pathophysiological processes of infection and inflammation in bovine mastitis and to investigate alternative therapies for mastitis.

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Section: Discussionsupporting

confidence: 93%

Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices

Filor

Seeger

Buhr

et al. 2022

Journal of Dairy Science

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“…Virulence factors of S. uberis are not well understood. Encapsulation and biofilm formation [ 23 , 24 , 25 , 26 , 27 , 28 ], adherence and invasion of mammary epithelial cells [ 12 , 14 ], virulence-associated immunogenic surface proteins [ 29 ], and other virulence factors [ 30 , 31 , 32 ] appear to be associated with the disease process. An exhaustive search for a single virulence factor as a vaccine candidate seems to be of limited success.…”

Section: Introductionmentioning

confidence: 99%

“…An exhaustive search for a single virulence factor as a vaccine candidate seems to be of limited success. Initial host- S. uberis interactions induce the expression of virulence-associated immunoreactive surface proteins [ 29 ]. Based on this finding, we believe that control of S. uberis mastitis could be achieved by using S. uberis surface-associated proteins (SUSP) as a vaccine to enhance the intramammary immunity more effectively than a single protein (subunit vaccine).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Streptococcus uberis Surface Proteins as Vaccine Antigens to Control S. uberis Mastitis in Dairy Cows

et al. 2021

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There is no effective vaccine against Streptococcus uberis mastitis in dairy cows. Objectives of this study were (1) to extract S. uberis surface proteins (SUSP) and determine immunoreactivity in vitro and (2) immunogenicity and efficacy in vivo. SUSP was extracted from S. uberis, and their immunoreactivity was tested by western blot. In total, 26 Jersey dairy cows were randomly divided into four groups. Groups 1, 2, and 3 were vaccinated subcutaneously with 4 mg, 1 mg, and 100 μg of SUSP, respectively, with Freund’s incomplete adjuvant. Group 4 (control) was injected with placebo. S. uberis UT888 was infused into two contralateral quarters of each cow during early lactation. Somatic cell count (SCC), bacteria count in milk, and mastitis were monitored. Our results show that SUSP contains multiple protein bands, that ranged from 10 to 100 kDa. All vaccinates showed an increased anti-SUSP IgG antibody. The SCC of all experimentally infected quarters increased after challenge but slightly decreased after day 3 with no significant difference among groups. Milk bacterial count was significantly (p < 0.05) reduced in high and medium doses vaccinated groups than low and control groups. In conclusion, SUSP vaccine is immunogenic and showed a promising efficacy to control bovine S. uberis mastitis.

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Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review

Fessia

Odierno

2021

Folia Microbiol

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Bovine intramammary infection associated immunogenic surface proteins of Streptococcus uberis

Cited by 10 publications

References 17 publications

Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices

Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices

Evaluation of Streptococcus uberis Surface Proteins as Vaccine Antigens to Control S. uberis Mastitis in Dairy Cows

Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review

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