Bovine Keratohyalin: Anatomical, Histochemical, Ultrastructural, Immunologic, And Biochemical Studies

Ugel, Arthur R.

doi:10.1111/1523-1747.ep12598085

Cited by 25 publications

(16 citation statements)

References 27 publications

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“…She stated that granules might be keratohyaline granules in nature. The studies on the histochemical and the chemical properties of keratohyaline granules (Van Scott 1951; Matoltsy 1962; Szodoray and Nagy 1964;Ugel 1975) have shown that they might be distinct from the peridermal granules. Jenson and Mottet (1970) reported the peridermal granules appeared in the skin culture of the five-day-old chick embryo and Voigt and Fusenig (1979) observed the appearance of these granules in the skin culture of newborn mice.…”

Section: Discussionmentioning

confidence: 99%

Peridermal granules of the chick embryo. Histochemical, ultrastructural and immuno-electron microscopical study.

Ito

Mori

1987

Tohoku J. Exp. Med.

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ITO, T. and MORI, T. Peridermal Granules of the Chick Embryo : Histochemical, Ultrastructural and Immuno-Electron Miroscopical Study. Tohoku J. exp. Med., 1987, 151(2), [169][170][171][172][173][174][175][176][177][178][179][180] In the chick embryo, the periderm covering certain derivatives of the integumentum, such as the beak, the scale and the claw, grows thick and contains specific cytoplasmic granules. In this report, we present the histochemical properties and the fine structures of these peridermal granules especially over the egg tooth region of the upper beak. The granules were brilliantly stained with acid dyes. A positive reaction for tyrosine and arginine suggested that this acidophilia was due to a certain basic protein. Although the reaction of DDD to SH and SS groups was moderate, a presence of keratin was strongly suggested immunohistochemically by using the rabbit anti-keratin antiserum. There also existed a possibility of some compound lipids. Under an electron microscope, these granules were found to consist of anastomosing ribbons which were less than 60 nm in width and were stained with rabbit anti-keratin antiserum. peridermal granules ; chick embryo ; histochemical property ; fine structure ; immunoelectron microscopic study The presence of acidophilic granules in the periderm has been known in the integumentum of sauropsid embryo (Rose 1892; Rosenstadt 1897). In the chick embryo, Branca (1907) described that these granules were found in the periderm especially over the intensely keratinizing regions of the skin such as the beak, the scale and the claw.These peridermal granules have attracted the notice of some researchers in relation to keratinization.For example, Voigt and Fusenig (1979) reported that they were a type of keratohyaline granules. In this report, the histochemical properties and the fine structures of these granules are presented, and the periderm covering the upper beak of the chick embryo is examined ultrastructurally immediately before hatching. In addition, the possibile relation of the peridermal granules to the differentiatiom of the epidermis is also discussed.

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Section: Discussionmentioning

confidence: 99%

Peridermal granules of the chick embryo. Histochemical, ultrastructural and immuno-electron microscopical study.

Ito

Mori

1987

Tohoku J. Exp. Med.

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“…The keratohyalin-granule-associated macroaggregates are histidine-rich proteins [17][18][19], In view of their presumable func tion in the differentiation process of the epidermis, they are designated as filament aggregating proteins [20][21][22], In a multistep process of phosphorylation and déphos phorylation, the histidine-rich alkaline pro teins become filament-aggregating proteins (filaggrin) [23,24], Protein analyses with the aid of the SDS-PAGE show that specifically retinoids with known antipsoriatic activity, such as et retinate and arotinoid ethylester [25,26], biochemically increase the synthesis of keratohyalin-granule-macroaggregates (his tidine-rich proteins), whereas retinoids with low antipsoriatic activity, such as all-rra/ts'-rctinoic acid and 13-m-retinoic acid [27,29], tend to inhibit this protein fraction (R;).…”

Section: Discussionmentioning

confidence: 99%

Retinoids and Keratinocyte Differentiation in vitro

Stadler

Müller²,

Detmar³

et al. 1987

Dermatology

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Standard retinoids (e.g. etretinate, isotretinoin) are successful in the treatment of a variety of dermatological disorders. However, they are handicapped by an unfavorable ratio between activity and toxicity. In order to find more successful substances new promising compounds were recently synthesized. The aim of this study was to screen new monoaro-mates (etretin (Ro 10–1670) , demethyletretin (Ro 12–7310), 13-cis-etretin(Ro 13–7652) and the polyaromates arotinoid (Ro 15–0778), arotinoid acid (Ro 13–7410) and arotinoid ethyl sulfone (Ro 15–1570) for their effect on keratinocyte differentiation in vitro and to compare these results with differentiation data of retinoids currently used clinically. It was found that the second generation of retinoids had a lower antikeratinizing potential than the third generation of retinoids. They markedly inhibited crosslinked keratins and envelope proteins. Arotinoid acid was found to be the most potent derivative. In contrast to this, differences with regard to the synthesis of keratohyalingranule macroaggregates were detected between the retinoid derivatives. Arotinoid acid and arotinoid sulfone stimulated this protein fraction, whereas arotinoid left this fraction uninfluenced. If the data obtained were considered the selection criterion for the future therapeutic application of the new arotinoids in psoriasis therapy, it should be more useful to employ arotinoid acid or arotinoid sulfone rather than arotinoid in clinical psoriasis studies.

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“…The proteins of the keratohyalin granules in the Stratum granulosum were extracted according to Ugel (1975) and Ugel and Idler (1972) from some of the samples (2 x 2 x 5 mm 3 ) that were obtained from the right hoof sections by incubating the specimens in a 1 molar solution of potassium phosphate (pH 7.0) for twelve hours. Subsequently, the specimens were rinsed in phosphate puffer and processed for the transmission electron microscopic study as described above.…”

Section: Chemistrymentioning

confidence: 99%

The role of the specific, profilaggrin-containing keratohyalin granules in the developing epidermis of the fetal horse hoof

Bragulla¹,

Homberger²

2007

PHK

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SummaryThe adult equine hoof is subdivided into distinct segments with various keratinization modes. In the periople and bulbs of the heel, the epidermis forms a Stratum granulosum with basophilic keratohyalin granules during soft keratinization, whereas in the coronet, wall proper, sole, and frog, the epidermis undergoes hard keratinization by keratinizing and cornifying without forming keratohyalin granules. The present study tests the hypothesis that the presence of specific (profilaggrin-containing) keratohyalin granules in the hoof epidermis is correlated with the water-binding capacity and mechanical properties of the hoof horn. To identify these specific profilaggrin-containing keratohyalin granules, tissue samples of fetal hooves were studied with histochemistry, immunohistochemistry, and transmission electron microscopy. In a fetal hoof, a Stratum granulosum is formed in all hoof segments in the wake of the establishment of a segment-specific papillary body, but at differing developmental stages, starting in the coronet, then in the wall proper, and later in the sole and frog, and disappearing again in the same sequence. In the terminal part of the wall proper (i.e., Zona alba), the Stratum granulosum is retained at least until three days after birth. In the periople and bulbs of the heel, the Stratum granulosum appears last (and is retained in the adult) when the other segments have not yet completely lost theirs. The basophilic granules in the Stratum granulosum are specific profilaggrin-containing granules that were also described in the human skin. These observations are relevant for a better understanding of certain dyskeratotic processes in the hoof epidermis.Keywords: Horse, hoof, development, horn, mechanical properties, epidermis, Stratum granulosum, keratohyalin, filaggrin, dyskeratosis, papillary body Zur Funktion der spezifischen, Profilaggrin enthaltenden Keratohyalingranula in der Hufepidermis des PferdefötusDer Huf des adulten Pferdes wird in Segmente eingeteilt, die einen unterschiedlichen Verhornungsmodus aufweisen. Im Saum-und Ballensegment bildet die Hufepidermis ein Stratum granulosum, welches lichtmikroskopisch durch basophile Keratohyalingranula gekennzeichnet ist. Dagegen verhornt die Epidermis im Kronsegment, im Wandsegment, in der Sohle und im Strahl ohne ein solches Stratum granulosum auszudifferenzieren. Das Stratum granulosum enthält in der Epidermis des Menschen mindestens zwei verschiedene Arten von Keratohyalingranula, die "spezifischen" Keratohyalingranula, welche Profilaggrin für den Filament-Matrix-Komplex der verhornenden Zellen enthalten, und die "unspezifischen" Keratohyalingranula, welche Proteine für eine zusätzliche Zellhülle der verhornenden Zellen enthalten. Die spezifischen Keratohyalingranula werden im Zuge des Verhornungsprozesses in der verhornenden Epidermiszelle durch Enzyme abgebaut. Die bei diesem enzymatischen Abbauprozess aus den Profilaggrinmolekülen entstehenden Filaggrinmoleküle durchdringen die Keratifilamentbündel und bilden auf diese Weise einen ...

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Bovine Keratohyalin: Anatomical, Histochemical, Ultrastructural, Immunologic, And Biochemical Studies

Cited by 25 publications

References 27 publications

Peridermal granules of the chick embryo. Histochemical, ultrastructural and immuno-electron microscopical study.

Peridermal granules of the chick embryo. Histochemical, ultrastructural and immuno-electron microscopical study.

Retinoids and Keratinocyte Differentiation in vitro

The role of the specific, profilaggrin-containing keratohyalin granules in the developing epidermis of the fetal horse hoof

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