Bovine Milk Lymphocytes Display the Phenotype of Memory T Cells and Are Predominantly CD8+

Taylor, Bernadette; Dellinger, J. D.; Cullor, James S; Stott, Jeffrey L

doi:10.1006/cimm.1994.1169

Cited by 90 publications

(83 citation statements)

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“…The mean percentage of CD21 + CMC was much lower than the percentage of B-lymphocytes found in bovine colostrum in other studies (Park et al, 1992;Kiku et al, 2010). Our results concerning B-lymphocytes agree with others (Taylor et al, 1994;Harp et al, 2004), as T-lymphocytes traffic selectively into bovine mammary gland secretions whereas B-lymphocytes represent a minor population as compared with peripheral blood. In general, the composition of the lymphoid compartment in the lactating mammary gland differs from that in peripheral blood, as demonstrated in the present and other studies (Park et al, 1992;Wirt et al, 1992;Van Kampen et al, 1999).…”

Section: Discussionsupporting

confidence: 89%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum β-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a 3 H-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum β-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen-and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum β-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.

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Section: Discussionsupporting

confidence: 89%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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“…In normal udder secretions, at least twice as many T-lymphocytes express the αβ receptor than the γδ receptor, are predominantly CD8+, and display the phenotype of memory cells [130,175,188].…”

Section: Cellular Defensesmentioning

confidence: 99%

Innate immunity of the bovine mammary gland

2006

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-Understanding the immune defenses of the mammary gland is instrumental in devising and developing measures to control mastitis, the major illness of dairy ruminants. Innate immunity is an extremely broad field for investigation, and despite decades of research, our present knowledge of the innate defenses of the udder is incomplete. Yet, information is being gained on the recognition of pathogens by the mammary gland, and on several locally inducible defenses. The contribution of mammary epithelial cells to local defenses and to the mobilization of leucocytes is under growing scrutiny. Interactions of mastitis-causing bacteria such as Escherichia coli or Staphylococcus aureus and the mammary gland represents a suitable model for studies on innate immunity at an epithelium frontier. Powerful new research tools are radically modifying the prospects for the understanding of the interplay between the mammary gland innate defenses and mastitis-causing bacteria: genetic dissection of the immune response, microarray gene technology, transcriptomic methodologies and gene silencing by RNA interference will make possible the discovery of several of the key defense mechanisms which govern the susceptibility/resistance to mastitis at the molecular and genetic levels. It should then be possible to enhance the resistance of dairy ruminants to mastitis through immunomodulation and genetic improvement. mastitis / cattle / innate immunity / inflammation / milk

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“…These cells can develop memory, resulting in a faster and more pronounced response after a 2nd or 3rd contact. Milk lymphocytes of healthy cows indeed display the phenotype of memory/activated cells [39,48].…”

Section: General Schedule Of Events During J5 Vaccinationmentioning

confidence: 99%

“…Actual data on bovine lymphocyte homing are, however, scarce. Milk lymphocytes display the phenotype of memory cells [39,48]. The percentage of memory cells in milk was up-regulated following subcutaneous S. aureus α-toxin immunization [39].…”

Section: Homing Of Memory T Cells Towards the Mammary Glandmentioning

confidence: 99%

Potential mechanism of action of J5 vaccine in protection against severe bovine coliform mastitis

2002

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-Coliform mastitis is one of the most difficult diseases to treat in the modern dairy industry. Curative therapy with antibiotics remains only moderately effective and depends on the stage at which the disease is treated. The most successful strategies for combating coliform mastitis appear to be prevention by hygienic management or prophylactic immunization. The severity of clinical symptoms of coliform mastitis has been shown to be reduced by immunization with the Escherichia coli J5 vaccine. However, although the J5 vaccine has been licensed in the United States for about 10 years, the immunological basis of its mechanism of action is still unknown. Until now, protection by J5 vaccination has often been explained by a straightforward mechanism of enhanced antibody production resulting in increased opsonization of coliform bacteria and lipopolysaccharides (LPS). The possibility that J5 vaccination could decrease risk factors for coliform mastitis such as impaired blood polymorphonuclear neutrophil leukocyte (PMN) diapedesis has never been investigated. This review provides arguments to support the hypothesis that J5 vaccination may reduce the severity of coliform mastitis by inducing a condition of mammary gland hyper-responsiveness, characterized by a T helper 1 (Th1) response and mediated by memory cells inside the mammary gland, finally resulting in enhanced PMN diapedesis upon an intramammary infection. dairy cow / coliform mastitis / J5 vaccine / T cell / immune response Résumé -Mécanismes d'action possibles du vaccin J5 dans la protection contre la mammite coliforme sévère de la vache laitière. La mammite coliforme est une des maladies les plus difficiles à traiter dans l'industrie laitière moderne. La thérapie curative avec des antibiotiques reste peu efficace, dépendant du stade de la maladie. Les stratégies les plus efficaces pour combattre la mammite semblent être la prévention par des mesures d'hygiène ou la prophylaxie vaccinale. La sévérité des symptômes cliniques de la mammite coliforme peut être réduite par immunisation avec le vaccin Escherichia coli J5. Cependant, alors que le vaccin J5 a été mis sur le marché aux États-Unis depuis (32) 9 2647321; fax: (32) 9 2647499; e-mail: christian.burvenich@rug.ac.be environ 10 ans, les bases immunologiques de son mécanisme d'action ne sont pas connues. Jusqu'à présent, la protection par le vaccin J5 a souvent été attribuée à un simple mécanisme de stimulation de la production d'anticorps résultant en une augmentation de l'opsonization des bactéries coliformes et des lipopolysaccharides (LPS). La possibilité que la vaccination avec J5 pourrait diminuer les facteurs de risque de la mammite coliforme, tels qu'une diapédèse insuffisante des leucocytes neutrophiles polymorphonucléaires (PMN) sanguins, n'a jamais été étudiée. Cet article de synthèse cite des arguments supportant l'hypothèse que la vaccination avec J5 pourrait réduire la sévérité de la mammite coliforme en induisant une hyper-sensibilité de la glande mammaire. Celle-ci serait caractér...

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Bovine Milk Lymphocytes Display the Phenotype of Memory T Cells and Are Predominantly CD8+

Cited by 90 publications

References 0 publications

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Innate immunity of the bovine mammary gland

Potential mechanism of action of J5 vaccine in protection against severe bovine coliform mastitis

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