Bovine Papillomavirus Type 2 Infection and a Series of Mesenchymal Tumors of the Urinary Bladder in Cattle

Abstract

This report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. Approximately 80% were hemangiomas and angiosarcomas. Hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. Although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequent malignant vascular tumors. Hemangiopericytoma and tumors of muscle and soft connective tissue origin, alone and/or in association with tumor-like lesions, were less frequently seen. Furthermore, forty-five cases of intravascular papillary endothelial hyperplasia (IPEH), a lesion not previously reported in the urinary bladder of cattle, were also described. Bovine papillomavirus type-2 DNA was amplified in tumor samples. Forty vascular tumors were investigated by dual-labeling immunofluorescence, and, for the first time, a coexpression of E5 and platelet-derived growth factor β receptor (PDGFβR) was shown to occur. The results show that the BPV-2 E5 oncoprotein binds to the activated form of the PDGFβ receptor thus playing an important role in mesenchymal as well as epithelial carcinogenesis of the urinary bladder. Furthermore, these findings demonstrate that BPV-2 infects both epithelial and mesenchymal cells.

Molecular identification of bovine papillomaviruses in dairy and beef cattle: first description of Xi- and Epsilonpapillomavirus in Turkey

“…In the epidermis, mild to severe acanthosis, hyperkeratosis, prominent parakeratosis, mild to moderate basophilic cytoplasmic swelling, vacuolar degeneration of keratinocytes, and many koilocytes (including keratohyalin granules) were present. Moreover, similar histopathologic features in BPV infection have been previously reported in cattle from Brazil (15,23,24) and from Italy (17,25), and in water buffaloes and cattle from Turkey (19)(20)(21) and from India (26).…”

Papillomaviruses in ruminants: An update

“…Considering the ruminant PV genomes, the Delta PVs are more often involved in malignant lesions hitherto (Borzacchiello & Roperto, ; Martano et al., ; Roperto, Munday, Corrado, Goria, & Roperto, ). In cattle, the BPVs 1 and 2 are the most frequently reported in urothelial bladder tumours worldwide (Borzacchiello & Roperto, ; Martano et al., ; Roperto, Munday, et al., ; Roperto, Russo, et al., ).…”

“…Considering the ruminant PV genomes, the Delta PVs are more often involved in malignant lesions hitherto (Borzacchiello & Roperto, ; Martano et al., ; Roperto, Munday, Corrado, Goria, & Roperto, ). In cattle, the BPVs 1 and 2 are the most frequently reported in urothelial bladder tumours worldwide (Borzacchiello & Roperto, ; Martano et al., ; Roperto, Munday, et al., ; Roperto, Russo, et al., ). Recently, the involvement of BPVs 13 and 14 as causative agents of urinary bladder tumours in cattle grazing on pasture containing bracken fern has been suggested through detection of their genome in single‐ and co‐infections or evidence of their E5 expression in affected cattle from Italy (Roperto, Munday, et al., ; Roperto, Russo, et al., ; Roperto, Russo, Ozkul, Corteggio, et al., 2013).…”

“…have been detected and characterized into Bovidae, Cervidae and Giraffidae families. This scenario can lead to economic losses mainly in countries that have vast amount of cattle herds, as BPV induces chronic, tumoral and contagious diseases that affects dairy and beef cattle herds worldwide and is associated with a variety of benign and malignant tumours (Araibi, Marchetti, Ashrafi, & Campo, 2004;Campo, 1997;Campo, Jarrett, Barron, Neil, & Smith, 1992;Da Silva et al, 2015;Jarrett, Campo, Neil, Laird, & Coggins, 1994;Martano et al, 2013;Ogawa et al, 2004). BPV is also highly important as an in vivo model for human papillomavirus (HPV) studies beyond veterinary interest (Campo, 1997;Gil da Costa & Medeiros, 2014).…”

Intestinal Adenocarcinoma in a Herd of Farmed Sika Deer (Cervus nippon)

“…19 While there was no histopathologic evidence of papilloma 44 or herpes virus 12 infection, its potential causative role cannot be ruled out without further molecular analysis. 27 In conclusion, the present study is the first to report intestinal adenocarcinoma in Sika deer. The disease occurred at an unusually high incidence in a single herd, and the degree of uniformity in tumor morphology differs markedly from findings in other animal species, suggesting a novel syndrome.…”