Bovine Paraoxonase 1 Activities in Serum and Distribution in Lipoproteins

Abstract

ABSTRACT. Paraoxonase 1 (PON1) is an enzyme associated with high-density lipoprotein (HDL) and has a protective effect against oxidation of lipoproteins in mammals. We investigated PON1 enzyme activities in bovine serum and its distribution among bovine serum lipoproteins. Paraoxonase activity and arylesterase activity in serum (152 Holstein cows and 42 Japanese Blacks) were 275 ± 55 U/ml and 130 ± 27 U/ml (mean ± SD), respectively. There was a high correlation (r=0.962) between the two enzyme activities, and the activity ratio of paraoxonase/arylesterase did not exhibit individual variation. More than 85% of both paraoxonase and arylesterase activities were detected in the HDL fraction separated by ultracentrifugation. The 43-kDa protein in the HDL fraction was identified as bovine PON1 by N-terminal amino acid sequence analysis. Bovine PON1 was purified by ultracentrifugation and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and an anti-bovine PON1 antiserum was developed. The concentration of PON1 protein determined by immunoblotting was closely correlated (r=0.976) with paraoxonase activity in serum. Bovine HDL was further fractionated into subpopulations, and the distribution of PON1 was examined. Paraoxonase activity and PON1 protein increased with decreasing HDL size and approximately 60% of total paraoxonase activity was distributed in the heavy HDL fraction. The different distributions of PON1 among HDL subpopulations might be concerned to the function and metabolism of bovine HDL. KEY WORDS: arylesterase, cattle, high-density lipoprotein, paraoxonase.

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

“…3). There was also a significant correlation between PON1 and ARE (r=0.96, P<0.01) like the report of Miyamoto et al [28] . On the other hand, there were no correlations between activities of PON1 or ARE and TAS or HDL-C levels (P>0.05).…”

Serum paraoxonase 1 (PON1) activity and lipid metabolism parameters changes in different production cycle periods of Holstein-Friesian, Polish Red and Norwegian breeds

“…There was a significant correlation between paraoxonase and arylesterase activity values in each period, with the highest value in the lactation peak. This might indicate that there is no polymorphism that can be distinguished by difference in P/A ratio in the studied serum samples, as well as no polymorphism or trimodal pattern was seen in Holstein-Friesian and Japanese Black cows (Miyamoto et al 2005).…”

A review of paraoxonase 1 properties and diagnostic applications

“…The bovine PON1 paraoxonase/arylesterase ratio in Holstein-Friesian, Polish Red and Norwegian cattle was constant suggesting there is no trimodal distribution like in humans and rabbits, when using two enzymatic activities towards paraoxon and phenyl acetate (Kulka et al 2014, Kulka et al 2016. Also no trimodal pattern was observed in Japanese Black breed where both activities where correlated (Miyamoto et al 2005). …”

“…Less than about 5% of PON1 activity for both substrates was found in very low density lipoprotein (VLDLs) and low density lipoprotein (LDLs) fractions. In the Holstein Friesian, Japanese Blacks, Swiss Blacks and Montofon bovine breeds PON1 was identified as 43 kDa protein (Miyamoto et al 2005, Erzengin et al 2014.…”