The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

Abstract

Reactive oxygen species have fundamental roles in reproductive functions. To comprehensively evaluate the relation between reactive oxygen species and infertility, physiological variations across the estrous cycle in healthy cows have to be known. For this purpose 25 healthy multiparous Holstein dairy cows having regular estrous cycles were used. The estrous cycles were synchronized by ovsynch protocol. Oxidant [lipid hydroperoxide (LOOH), total oxidant status (TOS), oxidative stress index (OSI)], antioxidant parameters [total antioxidant status (TAS), total free sulfhydryl groups (SH), ceruloplasmin (CP), paraoxonase-1 (PON1), arylesterase (ARE), uric acid (UA)], lipid profile and progesterone levels were assayed at estrus, metestrus, diestrus and proestrus stages of the estrous cycle in the plasma samples. The plasma levels of oxidant (LOOH, TOS and OSI) and antioxidant (TAS, SH and UA) parameters were significantly decreased during the luteal phase compared to the follicular phase (at proestrus and at estrus) of the estrous cycle. There was also a significant positive correlation between TAS and TOS. The activity of PON1 and ARE significantly increased only at diestrus. Levels of high density lipoprotein, low density lipoprotein and total cholesterol elevated during the follicular phase (estrus) and declined during the luteal phase. In conclusion, oxidant/antioxidant status and lipid profile were affected by cyclic changes. Moreover, antioxidant defense system showed adaptive response to increased oxidative activities by occurring parallel increases and it may indicate that there is a dynamic balance between oxidant and antioxidant status during the estrous cycle in healthy cows. Keywords: Cow, Estrous cycle, Lipid peroxide, Lipid profile, Total antioxidant status, Total oxidant status Sütçü İneklerde Östrus Siklusunun Oksidan ve Antioksidan Parametreler Üzerine Etkisi ÖzetReaktif oksijen türlerinin üreme fonksiyonları üzerinde önemli rolleri vardır. Sağlıklı süt sığırlarında reaktif oksijen türleri ve infertilite arasındaki ilişkiyi kapsamlı bir şekilde değerlendirmek için östrus siklusu boyunca reaktif oksijen türü seviyelerindeki değişimlerin bilinmesi gerekir. Bu çalışmada düzenli siklus gösteren 25 adet sağlıklı Holştayn ırkı sütçü inek kullanıldı. Östrus siklusları ovsynch protokolü ile senkronize edildi. Östrus siklusunun proöstrus, östrus, metaöstrus ve diöstrus dönemlerinde kan plazmasında oksidan parametreler [lipit hidroperoksit (LOOH), total oksidan seviye (TOS), oksidatif stres indeksi (OSI)], antioksidan parametreler [total antioksidan kapasite (TAS), total serbest sülfhidril gruplar (SH), seruloplazmin (CP), paraoksonaz-1 (PON1), arilesteraz (ARE), ürik asit (UA)], lipit profili ve progesteron seviyesi ölçüldü. Foliküler dönemle karşılaştırıldığında luteal dönemdeki plazma oksidan (LOOH, TOS, OSI) ve antioksidan (TAS, SH, UA) seviyelerinin önemli derecede düştüğü görüldü. Ayrıca TAS ve TOS değerleri arasında önemli bir pozitif korelasyon vardı. Paraoksonaz-1 ve ARE aktiviteleri sadece diöstrus ...

Kısa Süreli PRID Uygulanan Düvelerde Bazı Oksidatif Stres Parametrelerinin Değerlendirilmesi

“…For example, Aksu et al [22] conducted a study on cows in which the use of CIDR in the synchronization protocol resulted in higher MDA levels than in those cows for which the devices were not used. Other studies conducted on cattle found that TAC and TOC levels were higher during the period of the cycle which had high progesterone levels than they were during the estrus period [3,23] . It has also been reported that estrogen and progesterone can affect OS parameters in the body [2] .…”

“…Aydilek et al [3] conducted a study in which they attempted to measure TAC and TOC levels at various points of the reproductive cycle in cows. The study identified an increase in both factors during the estrus period.…”

“…The body consists of about 1-2% ROM, which play a role in many body activities [1] . These metabolites are found in low concentrations and play a role in functions such as protein phosphorylation, cell maturation, apoptosis, oocyte maturation, steroidogenesis, cell immunity, ovulation, implantation, blastocyst formation, luteolysis, acrosome reactions, fertilization and luteal maintenance during pregnancy [1][2][3][4] . However, when ROM is produced in large amounts in an organism, it causes damage to lipids, protein and DNA in the cell, resulting in the loss of cell functions.…”

Evaluation of some oxidative-stress and antioxidant markers in goats during estrous cycle under Egyptian environmental conditions

“…The highest RT was in phase IV, while the lowest was in phase II, and there was a significant interaction between estrus phases and hot conditions to exert this significant amendment. The estrous cycle is related to many physiological, metabolic, and hormonal variations (Aydilek et al, 2014), leading to diverse changes according to the estrus stage. The rise in RT during phase IV compared with phase II is explained by the corpus luteum that is fully functional during this stage and the increase in blood concentration of some hormones.…”

The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

“…Oxidative and antioxidative parameters were measured in the tissue samples using the Aeroset automated analyzer (Abbott, IL, USA) and a spectrophotometer (Cecil 3000, Cambridge, UK). The lipid hydroperoxide level (LOOH) was evaluated by the fluorometric method based on the reaction between malondialdehyde (MDA) and thiobarbituric acid [18]. All analyses were made in the Biochemistry Laboratory of the Faculty of Medicine at Harran University.…”