Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection

Almerı́a, S.; Castellà, Joaquim; Ferrer, D.; Ortuño, Anna; Estrada-Peña, Agustı́n; Gutiérrez, Josefina

doi:10.1016/s0304-4017(01)00464-2

Cited by 122 publications

(49 citation statements)

References 21 publications

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“…The morphological and biometrical parameters such as shape and site location of parasite in any infected erythrocyte have been considered for differential diagnosis (19)(20).…”

Section: Geimsa Stainingmentioning

confidence: 99%

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Molecular identification of ovine Babesia spp. in north of Iran

et al. 2013

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Background: Babesia is a blood-tissue parasite, which is transmitted by hard ticks from Ixodidae family. The parasite is the cause of babesiosis among small ruminants, cattle, human, dogs and other animals. Babesia is one of the main fatal factors among livestock in endemic regions such as Iran. The aim of this study was to identify Babesia spp infection using microscopic and molecular methods among small ruminants in Mazandaran and Golestan provinces, northern Iran, during 2011-2012. Materials and Methods:In this study, a total of 220 sheep and goats were selected from 22 flocks in different regions of these provinces and blood samples were taken from their ears. The samples were transferred to the laboratory. Then thick and thin smears were prepared, stained with Geimsa and examined under light microscope. Standard PCR and semi nested-PCR was performed to differentiate genus of Theileria and Babesia, also identify the species of Babesia. Results: From a total of 220 blood samples (160 sheep and 60 goats), 34 cases (15.4 %) showed Babesia infection using microscopic examination. Whereas, 11 cases (5%) were found positive for Babesia spp using standard PCR. Also, two positive cases were showed mixed infection with Theileria spp. In addition, two microscopic negative samples were positive by PCR assay.Using semi nested-PCR, Babesia ovis (n=10) and B. motasi (n=1) were detected. Conclusion: Our results shows ovine babesiosis is common in the Northern provinces of Iran. Moreover, Babesia ovis is the main causative agent of ovine babesiosis in northern Iran. The relatively high prevalence of Babesia infection in sheep and goats indicates the epizootic stability status of babesiosis in the northern part of Iran.

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“…The morphological and biometrical parameters such as shape and site location of parasite in any infected erythrocyte have been considered for differential diagnosis (19)(20).…”

Section: Geimsa Stainingmentioning

confidence: 99%

“…Babesia and theileria genomic DNA was extracted from suspected sheep and goat blood samples as described previously (19). Phenol chloroform isoamylalcohol method used for extracting DNA.…”

Section: Dna Extractionmentioning

confidence: 99%

Molecular identification of ovine Babesia spp. in north of Iran

et al. 2013

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“…The use of alternative techniques, such as DNA amplification methods, has become necessary to detect and identify Theileria infections effectively and has been reported in numerous recent studies. Molecular techniques are more sensitive and specific than other traditional diagnostic methods (d 'Oliveira et al 1995;Almeria et al 2001). Although many analyses were previously performed with the ticks' salivary gland smears stained with Methyl-green-pyronin or Feulgen staining, but the transmitter agent remained unanswered.…”

Section: Introductionmentioning

confidence: 99%

Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran

Yamchi

Tavassoli

2014

J Parasit Dis

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Tropical theileriosis is a progressive bovine lymphoproliferative disease caused by the intracellular protozoan parasite Theileria annulata. In this study 138 blood samples and 289 ticks were collected and examined from cattle that belonged to 10 randomly selected flocks. The Tbs-S/Tbs-A primer set was used for PCR amplification of Theileria spp. and the Ta-S/Tbs-A specific primer set was used in semi-nested PCR technique for detection of T. annulata. Blood smears of each case were examined by Giemsa staining method. The semi-nested PCR accurately revealed 22 (15.94 %) positive samples; whereas Giemsa staining method could detect 15 (10.86 %) out of 138 blood samples. The examination of 289 ticks by semi-nested PCR revealed that, 32.86 % of Hyalomma anatulicum anatulicum, 26.47 % of Hyalomma anatulicum excavatum and 22.42 % of Hyalomma asiaticum asiaticum, were infected with T. annulata. The results suggest that H. anatulicum anatolicum may play a major role in transmission of T. annulata infection in Iran. The results indicated that the Giemsa staining method, having low sensitivity, while the semi-nested PCR technique can be used as a gold standard method for this purpose.

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“…et al, 1993;Smeenk et al, 2000;Almeria et al, 2001;Oliveira-Sequeira et al, 2005;Liu et al, 2007;Martins et al, 2008;Sibeko et al, 2008). These techniques, however, rely on the availability of expensive equipment such as thermocyclers.…”

Section: Introductionmentioning

confidence: 99%

“…Although microscopic examination of thin blood smears has traditionally been used for the diagnosis of acute Babesia infections, it remains challenging to diagnose carrier animals using microscopic examinations due to the low sensitivity of the technique (Almeria et al, 2001). Several serological methods are available, but disadvantages include the occurrence of cross-reactions and the lack of discrimination between previous exposure and current infections (Wagner et al, 1992).…”

Section: Introductionmentioning

confidence: 99%