Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010

Badaracco, Alejandra; Garaicoechea, Lorena; Rodriguez, D.; Uriarte, Enrique Leopoldo Louge; Odeón, Anselmo; Bilbao, G.; Galarza, R.; Abdala, Alejandro Ariel; Férnandez, Francisco Tomás González; Parreño, Viviana

doi:10.1016/j.vetmic.2011.12.011

Cited by 45 publications

(137 citation statements)

References 25 publications

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“…), and viruses (rotavirus and coronavirus) (Oliveira Filho et al 2007, Stipp et al 2009, Izzo et al 2011, Blanchard 2012. Bovine group A rotavirus (BoRVA) is one the most important viral agents of neonatal diarrhea in calves worldwide , Badaracco et al 2012, Midgley et al 2012. The rotavirus belongs to the Reoviridae family and Rotavirus genus, and is composed of a triple-layered protein capsid.…”

Section: Introductionmentioning

confidence: 99%

Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

2014

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The episodes of diarrhea caused by neonatal bovine rotavirus group A (BoRVA) constitute one of the major health problems in the calf rearing worldwide. The main G (VP7) and P (VP4) genotypes of BoRVA strains involved in the etiology of diarrhea in calves are G6P[1], G10P[11], G6P[5], and G8P[1]. However, less frequently, other G and P genotypes have been described in BoRVA strains identified in diarrheic fecal samples of calves. This study describes the identification and molecular characterization of an emerging genotype (G6P[11]) in BoRVA strains involved in the etiology of a diarrhea outbreak in beef calves in a cattle herd of high production in extensive management system. The diarrhea outbreak, which showed high morbidity (60%) and lethality (7%) rates, occurred in calves (n= 384) Nelore (Bos indicus) up to 30-day-old from the State of Mato Grosso do Sul, Brazil. BoRVA was identified in 80% (16/20) of the fecal samples analyzed by polyacrylamide gel electrophoresis (PAGE) technique. In all PAGE-positive fecal samples were amplified products with 1,062-bp and 876-bp in the RT-PCR assays for VP7 (G type) and VP4 (VP8*) (P type) of BoRVA, respectively. The nucleotide sequence analysis of VP7 and VP4 genes of four wild-type BoRVA strains showed G6-III P[11]-III genotype/lineage. The G6P[11] genotype has been described in RVA strains of human and animal hosts, however, in calves this genotype was only identified in some cross-sectional studies and not as a single cause of diarrhea outbreaks in calves with high morbidity and lethality rates as described in this study. The monitoring of the G and P genotypes of BoRVA strains involved in diarrhea outbreaks in calves is important for both animal and public health by allowing the identification of the most frequent genotypes, the characterization of novel genotypes and to identify reassortments with genotypes described in animal and human hosts. The results of this study show the importance of the monitoring of the genotypes of BoRVA strains involved in episodes of bovine neonatal diarrhea as for characterization of frequency of occurrence and pathogenic potential of uncommon genotypes as for monitoring of the emergency of different BoRVA genotypes not included in commercial vaccines.

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Section: Introductionmentioning

confidence: 99%

Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

2014

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“…Rotavirus (RV) infections are the leading cause of neonatal diarrhea and are worldwide in distribution causing high morbidity and mortality in humans, animals and birds [1,4,14,21]. Among all serogroups, rotaviruses belonging to group A are predominant cause of severe gastroenteritis [4,26].…”

Section: Introductionmentioning

confidence: 99%

“…Among all serogroups, rotaviruses belonging to group A are predominant cause of severe gastroenteritis [4,26]. This non enveloped virus of family Reoviridae, having a double-stranded RNA (dsRNA) genome of 11 segments can exchange (reassort) genes [6,7,12] and the mechanism is very often in a setting where close proximity among human and animal exhibit, thus, aids for genetic reassortment [3].…”

Section: Introductionmentioning

confidence: 99%

Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India

et al. 2013

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Rotavirus (RV) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. Human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. Therefore, the present study was designed to explore RV incidence in a single approach from human and animal neonates sharing similar environment. Altogether, 200 diarrheal samples from children (50), piglets (80) and calves (70) were collected during the year of 2010-2012 from various locality, farms and hospitals, initially screened through monoclonal antibody based enzyme immunoassay followed by RNA-PAGE and VP7 gene amplification by Reverse transcription PCR. The overall prevalence of rotavirus was found to be 41.5 % (83/200) where maximum numbers of positive cases were found in piglets (46.3 %) followed by human (40 %) and cow (37.1 %). Majority of samples demonstrated characteristic group A rotavirus (RVA) electropherotype of 4:2:3:2 pattern. Moreover, RNA profiles of seven samples from piglets and calves revealed variation in the migration pattern of class II, III and class IV segments. The study, for the first time from the valley, detected 43.7 % of neonatal RVA positive cases from human and animal sharing similar setting. The variation in RNA migration pattern in seven cases signifies tentative cases of gene re-assortment that warrant further evaluation.

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“…The common bovine rotavirus strains are G6, G8 and G10. However, recent epidemiological studies in developing countries have shown increasing diversity of bovine rotaviruses (Minakshi et al, 2011 Badaracco et al, 2012;Malik et al, 2013Malik et al, , 2014. In addition, unusual G types, P types and G-P combinations have also been reported (Manuja et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Bovine group A rotavirus (BoRVA) is the main cause of diarrhea in neonatal calves causing severe economic loss due to morbidity and mortality in calves (Badaracco et al, 2012). The rotavirus genome consists of 11 segments of double-stranded RNA (dsRNA).…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of an Emerging G3P[3] Rotavirus Genotype in Buffalo Calves, India

Deswal¹,

Prasad²,

Ranjan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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The present study describes characterization of a novel rotavirus (B29) isolated from a buffalo calf causing severe diarrhea. It was confirmed by VP4 and VP7 gene based RT-PCR and in sequence analysis, the VP4 gene showed up to 99.3% sequence identity at nucleotide as well as amino acid levels with P[3] rotavirus genotypes. Similarly, VP7 gene showed a maximum nucleotide and amino acid identity of 99.1 and 98.7%, respectively with G3 genotypes of group A rotavirus (RVA) from several host species and places. The phylogenetic analysis of VP4 gene also revealed close relatedness with other P[3] genotype of rotaviruses from bovine, goat, canine and feline origin. Similarly, VP7 gene revealed close relation with several G3 rotaviruses from bovine and equine origin. The buffalo rotavirus isolate B29 was genotyped as G3P [3]. This is the first report of rotavirus isolate with a G3P[3] genotypic constellation in buffalo calvesfrom this region of India. The detection of buffalo rotavirus of G3P[3] genotypic combination reveals an unexpected epidemiological situation and diversity of bovine rotaviruses in India.

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Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010

Cited by 45 publications

References 25 publications

Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India

Genetic Characterization of an Emerging G3P[3] Rotavirus Genotype in Buffalo Calves, India

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