Bovine Sertoli Cells Colonize and Form Tubules in Murine Hosts Following Transplantation and Grafting Procedures

Zhang, Zhen; Hill, Jonathan R.; Holland, Michael; Kurihara, Yasuyuki; Loveland, Kate

doi:10.2164/jandrol.107.004465

Cited by 41 publications

(28 citation statements)

References 50 publications

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“…Our observation of the ability of Sertoli cells from young rats to form de novo tubule-like structures in the testicular interstitium is consistent with the previous reports of the development of testicular tubular structures when immature rodent, porcine, or bovine testicular cells, as pellets or following re-aggregation in culture, were implanted under the back skin or testis tunica albuginea of nude mice (Gassei et al 2006, Honaramooz et al 2007, Kita et al 2007, Zhang et al 2008.…”

Section: Discussionsupporting

confidence: 91%

“…Furthermore, although donor Sertoli cells often colonized at a high density, the number of spermatogonia in the tubules still appeared to be quite low. Similar to our present report, we found earlier that bovine Sertoli cells transplanted into nude mice either within the testis tubules or in the interstitium of the testis did not support bovine spermatogonial differentiation (Zhang et al 2008). Although others have observed that niches formed in mouse seminiferous tubules by transplantation of mouse Sertoli cells (Shinohara et al 2003) and in de novo formed tubules from testicular cell suspensions from both mice and rats transplanted subcutaneously (Kita et al 2007) did support spermatogenesis, this was only observed in a few tubules.…”

Section: Discussionsupporting

confidence: 84%

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Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis

et al. 2009

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Irradiation of rat testes leads to the failure to support differentiation of the surviving spermatogonia due to damage of the somatic environment. To determine the involvement of Sertoli cells in this somatic damage, we transplanted seminiferous tubule cells from normal immature GFP-transgenic rats into the testes of irradiated rats. The donor Sertoli cells colonized and developed in the host testes. In many seminiferous tubules, the donor Sertoli cells formed abnormal spherical structures in the lumen, but in some tubules they formed a normal-appearing epithelium, but with only isolated spermatogonia, on the basement membrane. When the donor cells were injected into the interstitial region of the testis, they formed tubule-like structures containing Sertoli cells and occasional isolated spermatogonia, both of donor origin. Surprisingly, in host tubules adjacent to these newly formed donor-cell tubules or adjacent to the endogenous tubules with abnormal donor Sertoli-cell structures, endogenous spermatogonia differentiated to the spermatocyte or even to spermatid stages. Around these newly donor cell-formed tubules and the host tubules with abnormal donor Sertoli-cell structures, many cells including macrophages, which perhaps represented chronic inflammation, accumulated in the interstitium. We conclude that the donor Sertoli cells that colonized the seminiferous tubules did not directly support recovery of spermatogenesis. Instead, the colonizing Sertoli cells acted indirectly on the interstitium to stimulate localized differentiation of endogenous spermatogonia.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 84%

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis

et al. 2009

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“…The PGP 9.5 positive cells were undifferentiated germ cells located in the basal compartment (Fig. 2a, b) [14,16].…”

Section: Discussionmentioning

confidence: 96%

“…Antibodies against PGP9.5 have been used for immunohistochemical detection of SSCs in several species such as: mouse [15], bovine [16], ram [14], humane [17] porcine [18]. This protein is readily observed in both the cytoplasm and nucleus of gonocytes and spermatogonia in bovine testes.…”

Section: Discussionmentioning

confidence: 99%

“…Antibody against this protein has been used for immunohistochemical detection of mouse [15], bovine [16], ram [14], human [17] and porcine [18] spermatogonia. Respecting to the lack of information on goat type A spermatogonial markers and the inadequate information regarding to the isolation, identification, and culture of goat SSCs, and considering the application of transfected SSCs as an efficient tool in production of transgenic animals, the present study was aimed to investigate the identification of prepubertal goat SSCs using polyclonal antibodies against PGP9.5 and c-kit molecular markers.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

Heidari

Rahmati-Ahmadabadi

Akhondi

et al. 2012

J Assist Reprod Genet

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Introduction Presently the techniques for making transgenic animals are cumbersome, required costly instruments and trained man-power. The ability of spermatogonial stem cells (SSCs) to integrate foreign genes has provided the opportunity for developing alternate methods for generation of transgenic animals. One of the big challenges in this field is development of the methods to identify and purify donor SSCs by antibody mediated cell sorting. Purpose The present study was aimed to identify goat subpopulations of SSCs using polyclonal antibodies against PGP9.5 and c-kit molecular markers as well as the growth characteristics of SSCs during short term culture. Methods One month old goats' testicular samples were subjected for immunohistochemical and immunocytochemical evaluations. The enzymatically isolated SSCs were cultured in DMEM plus FCS supplemented with (treatment) or without (control) growth factors (GDNF, LIF, FGF, and EGF) for 2 weeks. At the end of culture the morphological characteristics of SSCs colonies and immunocytochemical staining were evaluated. Results The number and size of colonies in treatment groups were significantly (P<0.01) higher than corresponding values in controls. The presence of PGP 9.5 and c-kit antigens was confirmed in immunocytochemical evaluation. In immunocytochemical evaluation, the proportion of c-kit and PGP9.5 positive cells were significantly (P<0.001) higher in control and treatment groups, respectively. Conclusions The presence of PGP9.5 and c-kit antigens was confirmed in goat SSCs. Moreover, culture medium supplementation with growth factors could effectively retain the undifferentiation status of SSCs, reflected as a higher population of PGP9.5 positive cells, after short term culture.

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Cell microarrays for the screening of factors that allow the enrichment of bovine testicular cells

et al. 2010

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Cell microarrays can serve as high-throughput platforms for the screening of a diverse range of biologically active factors and biomaterials that can induce desired cellular responses such as attachment, proliferation, or differentiation. Here, we demonstrate that surface-engineered microarrays can be used for the screening and identification of factors that allow the enrichment and isolation of rare cells from tissue-derived heterogeneous cell populations. In particular, we have focused on the enrichment of bovine testicular cells including type A spermatogonia and Sertoli cells. Microarray slides were coated with a copolymer synthesized from poly(ethylene glycol) methacrylate and glycidyl methacrylate to enable both the prevention of cell attachment between printed spots and the covalent anchoring of various factors such as antibodies, lectins, growth factors, extracellular matrix proteins, and synthetic macromolecules on printed spots. Microarrays were incubated with mixed cell populations from freshly isolated bovine testicular tissue. Overall, cell attachment was evaluated using CellTracker TM staining, whereas differential attachment of testicular cells was determined by immunohistochemistry staining with Plzf and vimentin antibodies as markers for type A spermatogonia and Sertoli cells, respectively. The results indicate that various surface immobilized factors, but in particular Dolichos biflorus lectin, allowed the enrichment of Plzf positive cells. Furthermore, Pisum sativum lectin, concanavalin A, collagen type IV, and vitronectin were identified as suitable negative selection factors. To our best knowledge, this work is the first to demonstrate the utility of surface engineered cell-based microarrays for the identification of factors that allow the selective capture of rare cells from tissue isolated heterogeneous mixtures. '

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Bovine Sertoli Cells Colonize and Form Tubules in Murine Hosts Following Transplantation and Grafting Procedures

Cited by 41 publications

References 50 publications

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis

Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

Cell microarrays for the screening of factors that allow the enrichment of bovine testicular cells

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