Bovine serum albumin adsorption to mica surfaces

Ellipsometric Study of Bovine Serum Albumin Adsorbed onto Ti/TiO2 Electrodes

“…By ellipsometry, however, Lassen and Malmsten (22) found that human serum albumin (HSA) adsorbs, leading to an adsorbed layer thickness of 17 Ϯ 3 nm under certain conditions, which is twice the size of the native form of the molecules in solution. A high layer thickness was also found for BSA adsorbed on mica surfaces as deduced from surface force measurements (30). In their study of adsorption of ovalbumin (OA) on metallic titanium, Ivarsson et al (28) reported that the protein formed a compact layer of about 1.2 nm thickness, which is smaller than the normal size of the OA molecule.…”

Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study

“…Further, the surface coverage value obtained for 2,4-D-OVA by SPR angle measurements is highly comparable with those reported for adsorption of albumins at liquid-air or solid-liquid interfaces by means of X-ray photoelectron spectroscopy, neutron reflection and ellipsometry measurements. Surface coverage values for adsorption of ovalbumin and bovine serum albumin were shown to vary from 1.8-5.0 mg/m 2 (180-500 ng/cm 2 ) depending on the ionic strength, pH and the nature of the interface [46][47][48][49], with high surface coverage values of 4.0-5.0 mg/m 2 (400-500 ng/cm 2 ) for adsorption on solid surfaces [48,49]. All these observations imply that the 2,4-D-OVA layer fabricated on SPR sensor chip by simple physical adsorption is a compact, closely-packed monomolecular layer of the conjugate.…”

Inlet protein aggregation: a new mechanism for lubricating film formation with model synovial fluids

“…The test fluid was 25 per cent w/w BS solution. The spectrum shows intense absorbance bands at~1650 and 1535 cm 21 that are characteristic of protein amide I (n C=O ) and II (n C-N , d N-H ) band vibrations. The IR reflection spectra confirm the deposits are proteins; however, the analysis can also provide insights as to the molecular conformation.…”

“…The formation of a precursor monolayer might then be followed by multilayer formation [22,24]. Proteins are also known to aggregate in solution [22], under shear [22], at surfaces [21], and after heat treatment [22,23], and these structures may be involved in the multilayer formation. The agglomerate can be large; for example, Santos et al [22] reported hydrodynamic radii of 3 nm for native species and 110-160 nm for aggregates.…”