Bovine serum albumin-directed synthesis of biocompatible CdSe quantum dots and bacteria labeling

Probing the interaction of flower-like CdSe nanostructure particles targeted to bovine serum albumin using spectroscopic techniques

“…Given the potential applications of CdSe in labels and probes, it is important to evaluate its influence on biomolecules. Though the interaction of CdSe QDs with biomolecules has been already studied clearly [23][24][25], there were few studies about the interaction of other morphologies of CdSe NP with BSA. Therefore, the influence of flower-like CdSe nanostructure particles on protein in real cells can be simulated in this study choosing BSA as a model.…”

Bio-templated CdSe quantum dots green synthesis in the functional protein, lysozyme, and biological activity investigation

“…According to the previous literatures [22,23,27,32], the appropriate chelating time between Lyz and Cd 2þ was important to achieve desirable results. Thus, a solution containing a suitable concentration of Cd 2þ and a 14.4 mg ml À1 aqueous solution of Lyz (5 ml) were mixed with vigorous stirring at room temperature, and then the mixed reaction solution must be kept static for 6 h under the same condition.…”

“…Furthermore, proteins may provide the possibility to produce materials with highly specific or multiple functions [26]. Compared to the numbers of studies in biomimetic syntheses of gold, silver, and platinum, protein-guided formation of QDs, is relatively underexplored [22,25]. QDs have been successfully prepared by using common protein [22e25,27] e.g., BSA.…”

Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis

“…Biomolecules were successfully functionalized on the QDs to detect bacteria. Briefly, TGA [73] and bovine serum albumin (BSA) [74] capped CdSe QDs were used as fluorescent probes for rapid detecting Escherichia coli and Staphylococcus aureus . The TGA functionalized CdSe QDs showed high degree of interaction with the amino group of bacterial cell membranes of E. coli and S. aureus and detected the bacteria at 10 2 –10 7 CFU/mL.…”