Bovine subclinical mastitis-associated methicillin-resistant Staphylococcus aureus, selective genotyping and antimicrobial susceptibility profile of the isolates in Kurdistan province of Iran

Abstract

Background and Objectives: Staphylococcus aureus is frequently involved in bovine subclinical mastitis worldwide. Besides, the methicillin-resistant S. aureus (MRSA) carrier state of animals is a matter of worrisome. This study aimed to evaluate the frequency of MRSA, discriminatory geno-analysis and antibiotic resistance scheme of the strains isolated from bovine subclinical mastitis in Kurdistan province of Iran.
 Materials and Methods: A total of 283 samples were collected and analyzed for S. aureus phenotypically and molecularly. SCCmec and coa types, and pvl gene were evaluated using polymerase chain reaction (PCR). Finally, the restriction fragment length polymorphism (RFLP) patterns of coa types and the antimicrobial susceptibility profile of the isolates were assessed.
 Results: Among the 95 isolates of S. aureus, 11 (11.57%) strains were recognized as MRSA. Six, one, and four SCCmec types represented for IVa, IVc, and V were determined, respectively, among which an individual IVa and V determinant harboured pvl gene. Restriction digestion products of 490 bp, 680 bp, and 730 bp of coa bands were generated. Tobramycin, mupirocin, fusidic acid, clindamycin, and chloramphenicol were the most effective drugs against the MRSA isolates.
 Conclusion: The detrimental involvement of S. aureus in bovine subclinical mastitis is proved herein. Besides, the contribution of MRSA and potential contamination of milk and dairy products with the bacterium may impose a serious public health risk. This demands serious and long-lasting efforts to control the infection. The results may be effective in the implementation of accurate controlling strategies.

Lactobacillus reuteri inhibits Staphylococcus aureus‐induced mastitis by regulating oxytocin releasing and gut microbiota in mice

“…Therefore, antibiotics have been considered to be the first choice in the treatment of this disease for centuries. However, the already available antibiotics for mastitis treatment are limited due to growing bacterial resistance and public health restrictions 1,2 . Therefore, it is particularly important to propose new effective preparations and methods for the prevention and control of mastitis.…”

Antibiograms, multidrug resistance, and milk-related parameters of bacteria isolated from milk of dairy cattle in Phatthalung, Thailand

“…Moreover, reduction in antibiotic use has been promoted globally [ 13 ], and antimicrobial resistance, particularly antibiotic resistance (ABR), is a global concern [ 14 ]. Remarkably, ABR in mastitis-causing bacteria is a source of concern [ 15 ]. The main cause of ABR in the dairy industry is the incorrect use of antibiotic doses, and ABR in mastitis medication has been identified as an important aspect of One Health Practice [ 16 ].…”

Multiple roles of LncRNA-BMNCR on cell proliferation and apoptosis by targeting miR-145/CBFB axis in BMECs

“… 2018 ), moreover, long-term antibiotic treatment could exacerbate the resistance of pathogenic bacteria and increase the hidden dangers caused by drug residues (Ngaywa et al. 2019 ; Khazaie and Ahmadi 2021 ). With the development of the next-generation sequencing technology, long noncoding RNAs (lncRNAs) are gradually recognized as an important epigenetic regulation method, and has been gradually used in the field of prevention and treatment of dairy cow mastitis.…”