Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense

Zilli, Jerri Édson; Baraúna, Alexandre Cardoso; Silva, Krisle da; Meyer, Sofie E. De; Farias, Eliane N. C.; Kaminski, Paulo Emílio; Costa, Ismaele Breckenfeld da; Ardley, Julie; Willems, Anne; Camacho, Natália N.; Dourado, Fernanda dos S.; O’Hara, G.W.

doi:10.1099/ijs.0.065458-0

Cited by 42 publications

(10 citation statements)

References 39 publications

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“…Furthermore, our data suggest that in the northeast of Brazil, B. neotropicale -like strains are the major symbionts of cowpea in soils with no previous cowpea cultivation. These bacteria were first isolated from the neotropical leguminous tree Centrolobium paraense and proven to effectively nodulate cowpea (Zilli et al, 2014). Interestingly, sequences of other rhizobial groups more rarely isolated from cowpea nodules, e.g., Microvirga vignae (Radl et al, 2014), were in very low abundance in the libraries and, hence, not detectable after the data filtering.…”

Section: Discussionmentioning

confidence: 99%

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

et al. 2017

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Many studies have been pointing to a high diversity of bacteria associated to legume root nodules. Even though most of these bacteria do not form nodules with legumes themselves, it was shown that they might enter infection threads when co-inoculated with rhizobial strains. The aim of this work was to describe the diversity of bacterial communities associated with cowpea (Vigna unguiculata L. Walp) root nodules using 16S rRNA gene amplicon sequencing, regarding the factors plant genotype and soil type. As expected, Bradyrhizobium was the most abundant genus of the detected genera. Furthermore, we found a high bacterial diversity associated to cowpea nodules; OTUs related to the genera Enterobacter, Chryseobacterium, Sphingobacterium, and unclassified Enterobacteriacea were the most abundant. The presence of these groups was significantly influenced by the soil type and, to a lesser extent, plant genotype. Interestingly, OTUs assigned to Chryseobacterium were highly abundant, particularly in samples obtained from an Ultisol soil. We confirmed their presence in root nodules and assessed their diversity using a target isolation approach. Though their functional role still needs to be addressed, we postulate that Chryseobacterium strains might help cowpea plant to cope with salt stress in semi-arid regions.

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Section: Discussionmentioning

confidence: 99%

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

et al. 2017

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“…The DNA G + C content estimated through the software JSpecies of strain BR 10245 T was 62.5 mol% and BR 10303 T was 62.4 mol%, both of which are within the range for Bradyrhizobium (Kuykendall et al 1992;Ramírez-Bahena et al 2009;Zilli et al 2014).…”

Section: Ani and Dna G + C Contentmentioning

confidence: 59%

“…In addition, the Bradyrhizobium strains showed a distinct phylogenetic position from currently described species. Further polyphasic analysis confirmed the presence of a novel species, and B. neotropicale was described (Zilli et al 2014). Although this new species belongs to sub-clade II within the Bradyrhizobium genus according to 16S rRNA gene sequencing, housekeeping genes (dnaK, glnII, recA, and rpoB) sequences confirmed that it is affiliated to sub-clade I (Zilli et al 2014).…”

Section: Introductionmentioning

confidence: 76%

Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil

et al. 2017

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Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity. Multi-locus sequence analysis (MLSA) with dnaK, glnII, recA, and rpoB confirmed B. neotropicale BR 10247 as the closest type strain for the group I and B. elkanii USDA 76 and B. pachyrhizi PAC 48 for group II. Average Nucleotide Identity (ANI) differentiated group I from the B. neotropicale BR 10247 (79.6%) and group II from B. elkanii USDA 76 and B. pachyrhizi PAC 48 (88.1% and 87.9%, respectively). Fatty acid profiles [majority C and Summed feature 8 (18:1ω6c/18:1ω7c) for both groups], DNA G + C content, and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae, and Phaseoleae. Based on the presented data, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed, with BR 10245 (=HAMBI 3597) and BR 10303 (=HAMBI 3602) as the respective-type strains.

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“…icense (Durán et al, 2014a,b), B. manausense (Silva et al, 2014a), B. ingae (Silva et al, 2014b), B. valentinum (Durán et al, 2014a,b), B. neotropicale (Zilli et al, 2014), B. ottawaense (Yu et al, 2014), B. erythrophlei and B. ferriligni (Yao et al, 2015). The number of described novel species has increased in the past two years along with the use of appropriate molecular techniques, such as housekeeping gene analysis (Willems et al, 2001;Martens et al, 2008;Rivas et al, 2009), as well as the study of new host plants and unexplored geographic regions.…”

Section: Introductionmentioning

confidence: 98%

“…These authors applied phenotypic (SDS-PAGE of total proteins) and genotypic methods (AFLP, IGS PCR-RFLP and Rep-PCR) that showed also high interspecific variability. In tropical regions, only recently the analysis of housekeeping genes sequences has been applied, allowing for the reliable proposal of new species (Silva et al, 2014a, b;Zilli et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic efficiency and genetic diversity of soybean bradyrhizobia in Brazilian soils

Ribeiro

Santos

Costa

et al. 2015

Agriculture, Ecosystems & Environment

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Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense

Cited by 42 publications

References 39 publications

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil

Symbiotic efficiency and genetic diversity of soybean bradyrhizobia in Brazilian soils

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