Brain acetylcholinesterase, malondialdehyde and reduced glutathione as biomarkers of continuous exposure of tench, Tinca tinca, to carbofuran or deltamethrin

This work presents the study of the effects of acute exposure to various concentrations of copper from 0.03 to 0.59 mol⋅L −1 on the metabolic activities (nitrogen and phosphorus), protein levels, catalase (CAT) enzyme activities, and the biological response malondialdehyde (MDA) in the mollusks Perna perna and Mytilus galloprovincialis. The concentrations above 0.88 mol⋅L −1 have proven to be lethal to P. perna. The results show slight disturbances of metabolism as a result of pollutant and a significant correlation between metal contamination and ammonia nitrogen levels, resulting in an increase in the latter after 48 h of M. galloprovincialis exposure to 0.59 mol⋅L −1 of copper concentrations. CAT was rapidly induced even by low concentrations of copper; MDA was increased only with low concentrations of copper in P. perna.

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“…They can be used to measure the interaction between a biological system and a chemical physical or biological environmental agent [18,19].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Effects in the BivalvePerna pernaandMytilus galloprovincialis: Impact on the Environment due to Contamination by Copper

Errahmani

Zouaoui

Bendjoudi

2014

Journal of Marine Biology

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“…Furthermore, once a week, water samples (V=250 mL) were taken from each tank and subjected to high-performance liquid chromatography (HPLC) analysis in order to assess the effective concentrations of the pesticide. Standard HPLC technique with acetonitrile and water as mobile phase and a Nucleosyl 120 C18 column was applied, according to the method previously described for other carbamate pesticides (11).…”

Section: Experimental Designmentioning

confidence: 99%

“…Comparison of the values measured in the exposed fish indicate that brain AChE activity was significantly lower on day 4 as compared to day 15 ( Figure 1). Several studies reported a similarity between the AChE activity of exposed fish and controls after an initial period of inhibition (11,40). Reactivations of the carbamate-mediated anticholinesterase effect, or even an enzyme superproduction as a response to inhibition, have been reported as cause for that phenomenon (41).…”

Section: Figure 1 Levels Of Catalase (Cat) Glutathione-s-transferasementioning

confidence: 99%

“…Moreover, Bretaud et al (40) reported a significant inhibition of both brain and muscle AChE activity in the goldfish (Carassius auratus) after 48 h of exposure to 500 mg L -1 of carbofuran. The same pesticide also caused AChE activity inhibition in tench (Tinca tinca) exposed to 100 μg L -1 of carbofuran, whereas an exposure to deltamethrin did not affect AChE activity (11). Li et al (4) evaluated the acute toxicity of the pesticide methomyl on the topmouth gudgeon (Pseudorasbora parva).…”

Section: Figure 1 Levels Of Catalase (Cat) Glutathione-s-transferasementioning

confidence: 99%

“…Brought to you by | MIT Libraries Authenticated Download Date | 5/9/18 3:16 PM Moreover, many of these potentially toxic compounds or their metabolites have shown toxic effects related to oxidative stress in fish (4,(9)(10)(11). Cells have a variety of defence mechanisms to neutralize the harmful effects of free radicals (10).…”

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confidence: 99%

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Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Hernández‐Moreno

Resino

Flores

et al. 2014

Archives of Industrial Hygiene and Toxicology

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This study investigated the influence of the pesticide methomyl on different enzymatic activities in carp. The fish were exposed to a sub-lethal concentration (0.34 mg L -1 ) of methomyl for 15 days. On days 4 and 15, catalase (CAT) and glutathione-S-transferase (GST) activities were measured in the liver and gills. Acetylcholinesterase (AChE) activity in brain and muscle was also determined. Liver catalase activity slightly increased in exposed fish when compared to controls, but it was statistically significant only at the beginning of the experiment. No changes in CAT activity in the gills of exposed and control animals were observed (mean values were in the range 10.7-11.7 nmol min -1 per mg of protein). Liver GST activity was slightly inhibited in the exposed animals at the beginning of the study; however, it was significantly inhibited in the gills. Brain AChE activity was diminished throughout the experiment and significantly decreased after 96 h of exposure compared to controls (0.041 vs. 0.075 nmol min -1 per mg of protein; p<0.001). Our findings suggest that CAT, GST, and AChE are reliable biomarkers of effect after exposure to methomyl.

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Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.)

Radovanović

Nasia

Krizmanić

et al. 2017

Enviro Toxic and Chemistry

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Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.

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Brain acetylcholinesterase, malondialdehyde and reduced glutathione as biomarkers of continuous exposure of tench, Tinca tinca, to carbofuran or deltamethrin

Cited by 66 publications

References 67 publications

Metabolic Effects in the BivalvePerna pernaandMytilus galloprovincialis: Impact on the Environment due to Contamination by Copper

Metabolic Effects in the BivalvePerna pernaandMytilus galloprovincialis: Impact on the Environment due to Contamination by Copper

Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.)

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