Brain‐derived neurotrophic factor prevents the endothelial barrier dysfunction induced by <scp>interleukin</scp>‐1β and <scp>tumor necrosis factor</scp>‐α

Matsuda, Shinji; Fujita, Tsuyoshi; Kajiya, Mikihito; Kashiwai, K.; Takeda, Katsuhiro; Shiba, Hideki; Kurihara, Hidemi

doi:10.1111/jre.12226

Cited by 17 publications

(9 citation statements)

References 61 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An in vitro study indicated that the addition of BDNF could attenuate apoptosis induced by TNF-α and IFN-γ in enteric glial cells (EGCs) (Steinkamp et al 2012). In human vascular endothelial cells, BDNF maintains the integrity of blood vessel walls and avoids vascular leakage by protecting cells from apoptosis in response to inflammatory mediators (Matsuda et al 2015, Takeda et al 2013). In addition, BDNF is highly expressed in colorectal cancer compared with non-tumor tissues, and increased expression of BDNF can inhibit spontaneous cell apoptosis in tumorigenesis (Brunetto de Farias et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice

Zhao

Zhang

Qi³

et al. 2018

Physiol Res

View full text Add to dashboard Cite

We aimed to investigate the effects of brain-derived neurotrophic factor (BDNF) on apoptosis of intestinal epithelial cells (IECs) and alterations of intestinal barrier integrity using BDNF knock-out mice model. Colonic tissues from BDNF +/+ mice and BDNF +/mice were prepared for this study. The integrity of colonic mucosa was evaluated by measuring trans-mucosa electrical resistance and tissue conductance in Ussing chamber. The colonic epithelial structure was analyzed by transmission electron microscopy.Apoptosis involvement was determined with TUNEL staining, active caspase-3 immunostaining and Western blotting for the protein expression of active caspase-3, Bax and Bcl-2. The expression levels and distribution of tight junction proteins were evaluated by immunohistochemistry or Western blots. Compared with BDNF +/+ mice, BDNF +/mice displayed impaired integrity and ultrastructure alterations in their colonic mucosa, which was characterized by diminished microvilli, mitochondrial swelling and epithelial cells apoptosis. Altered intestinal barrier function was linked to excessive apoptosis of IECs demonstrated by the higher proportion of TUNEL-positive apoptotic cells and enhanced caspase activities in BDNF +/mice. Increased expression of Bax and claudin-2 proteins and reduced Bcl-2 and tight junction proteins (occludin, ZO-1 and claudin-1) expression were also detected in the colonic mucosa of BDNF +/mice. BDNF may play a role in the maintenance of intestinal barrier integrity via its anti-apoptotic properties.

show abstract

Section: Introductionmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice

Zhao

Zhang

Qi³

et al. 2018

Physiol Res

View full text Add to dashboard Cite

show abstract

“…21 BDNF modulates cadherin expression and shedding 30,31 and interacts with tumor necrosis factor-α. 32,33 Both cadherin 34 and tumor necrosis-α 35-37 contribute to atrial structural remodeling in human and animal studies, and therefore changes in BDNF may promote development of a substrate vulnerable to AF. We hypothesized that BDNF is associated with incident AF, possibly through modulation of endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Serum brain-derived neurotrophic factor and risk of atrial fibrillation

Rahman

Himali

Yin

et al. 2017

American Heart Journal

View full text Add to dashboard Cite

Background and objective Brain-derived neurotrophic factor (BDNF) is expressed by endothelial cells and can affect cardiovascular function. We examined if serum BDNF was associated with risk of incident atrial fibrillation (AF) in the Framingham Heart Study. Methods We studied individuals without an AF diagnosis at baseline from the Framingham Original and Offspring cohorts. We used age- and sex-adjusted, and multivariable-adjusted Cox proportional hazard regression models to examine the association of serum BDNF concentrations with 10-year risk of incident AF. Results We studied 3457 participants (mean age 65±11 years, 58% women). During follow-up 395 participants developed AF. In unadjusted analysis, higher mean serum BDNF concentration was associated with lower incidence of AF (hazard ratio 0.89 per SD, 95% confidence interval 0.80 to 0.99). In multivariable-adjusted analyses, serum BDNF concentration was not significantly associated with incident AF (hazard ratio 0.98 per SD, 95% confidence interval 0.88 to 1.09). Compared with the lowest quartile, BDNF levels in the other quartiles were not associated with risk of AF in multivariable-adjusted analyses. No interactions between sex or age with serum BDNF concentrations and risk of AF were found. Conclusions In our prospective, community-based sample we did not find a statistically significant association of serum BDNF levels with risk of incident AF.

show abstract

“…Studies report that in human minor salivary glands, as claudin‐1 and claudin‐4 increase, ZO‐1 and occludin decrease [11]. Additionally, the expression of the epithelial tight junction proteins ZO‐1, occludin, claudin‐1 and claudin‐2 can be regulated by brain‐derived neurotrophic factor (BDNF) [12]. BDNF, a member of the neurotrophin polypeptide family, is abundantly and widely expressed in the nervous system [13].…”

Section: Introductionmentioning

confidence: 99%

Brain‐derived neurotrophic factor preserves intestinal mucosal barrier function and alters gut microbiota in mice

Cai

Zhang

2018

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

The intestinal mucosal barrier (IMB) enables the intestine to provide adequate containment of luminal microorganisms and molecules while preserving the ability to absorb nutrients. In this study, we explored the effect of brain-derived neurotrophic factor (BDNF) on IMB function and gut microbiota in mice. BDNF gene knock-out mice (the BDNF group) and wild-type mice (the BDNF group) were selected. The gut microbiota of these mice was analyzed by denaturing gradient gel electrophoresis (DGGE) assay. The ultrastructure of the ileum and the colonic epithelium obtained from decapitated mice were observed by transmission electron microscopy. The protein expression of epithelial tight junction proteins, zonula occludens-1 (ZO-1) and occludin was detected by immunohistochemistry staining. The protein expression of claudin-1 and claudin-2 was determined by Western blotting. The DGGE band patterns of gut microbiota in the BDNF group were significantly different from that in the BDNF group, which indicated that the BDNF expression alters the gut microbiota in mice. Compared with the BDNF group, the BDNF group presented no significant difference in the ultrastructure of ileal epithelium; however, a significant difference was observed in the colonic epithelial barrier, manifested by decreased microvilli, widening intercellular space and bacterial invasion. Compared with the BDNF group, the expression of ZO-1 and occludin in the BDNF group was significantly decreased. The expression of claudin-1 in the BDNF group was significantly reduced, while the expression of claudin-2 was elevated. These findings indicate that BDNF preserves IMB function and modulates gut microbiota in mice.

show abstract

Brain‐derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin‐1β and tumor necrosis factor‐α

Abstract: BDNF played an important role in inhibiting endothelial barrier dysfunction, which suggests that it may assist in enhancing periodontal tissue regeneration.

Cited by 17 publications

References 61 publications

Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice

Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice

Serum brain-derived neurotrophic factor and risk of atrial fibrillation

Brain‐derived neurotrophic factor preserves intestinal mucosal barrier function and alters gut microbiota in mice

Contact Info

Product

Resources

About