Brassica carinata CIL1 mediates extracellular ROS production during auxin- and ABA-regulated lateral root development

Gibson, Shawn; Conway, Alix J.; Zheng, Zhifu; Uchacz, Tina; Taylor, Janet L.; Todd, Christopher D.

doi:10.1007/s12374-011-0328-4

Cited by 9 publications

(7 citation statements)

References 93 publications

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“…Decreases in lateral root development were observed when expression of these genes was eliminated. Similar to the effects of reducing Brassica carinata CIL1, an orthologue of AIR12, root development in response to the hormones auxin and abscisic acid was altered in the mutants compared to control plants (Gibson et al 2012). The hormone treatments used act through discrete and separate pathways; however, the response to both hormones was attenuated in mutants.…”

Section: Discussion Germination and Root Growthmentioning

confidence: 85%

“…On medium containing 2,4-D however, root growth matched that of wild type seedlings. Despite the appearance of an auxin deficiency, the response of air12 and cil1 seedlings (Gibson et al 2012) to synthetic auxin did not match any described model, though it is worth noting that these studies utilized synthetic hormones, resulting in a plant response that may differ compared to the response to endogenous hormones.…”

Section: Discussion Germination and Root Growthmentioning

confidence: 94%

“…In addition, these proteins can be cleaved from the anchor by phospholipase C (Borner et al 2003), a protein often produced following an ABA-mediated abiotic stress response (Hunt et al 2003). BcCIL1 is released to the apoplast following osmotic shock (Gibson et al 2012) as is AIR12 (data not shown). The air12 seeds germinated faster than controls on all abiotic stress treatments examined (Fig.…”

Section: Discussion Germination and Root Growthmentioning

confidence: 99%

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Arabidopsis AIR12 influences root development

Gibson

Todd

2015

Physiol Mol Biol Plants

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Arabidopsis AUXIN INDUCED IN ROOTS (AIR 12) is a predicted to encode a glycosylphosphatidylinositol tail anchored protein. It has been associated with extracellular redox processes, but little is known about its physiological role. An air12 mutant line demonstrated increased germination rates in the presence of a range of abiotic stress factors and hormones, but not in the presence of ABA. Disruption of AIR12 also affected primary and lateral root development and was linked to changes in root catalase activity and superoxide production. We suggest AIR12 is an extracellular constituent linking both hormone and reactive oxygen signaling in plants.

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Section: Discussion Germination and Root Growthmentioning

confidence: 85%

Section: Discussion Germination and Root Growthmentioning

confidence: 94%

Section: Discussion Germination and Root Growthmentioning

confidence: 99%

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Arabidopsis AIR12 influences root development

Gibson

Todd

2015

Physiol Mol Biol Plants

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“…The relative rapid expression of AGPs under exogenous phytohormone treatments suggested that they may function in maintaining the organism's homeostasis rather than solely being structural components. In plants, sugar levels reflect the plant's physiological conditions and sugar signaling is closely associated with multiple phytohormone signaling cascades and abiotic stress signaling (Smeekens, 2000; Gibson, 2005; Rolland et al, 2006). Interestingly, sugar can induce ABA in the sugar-ABA signaling cascade (Laby et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Genomic, Molecular Evolution, and Expression Analysis of Genes Encoding Putative Classical AGPs, Lysine-Rich AGPs, and AG Peptides in Brassica rapa

Han¹,

Dong²,

Cui³

et al. 2017

Front. Plant Sci.

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Arabinogalactan proteins (AGPs) belong to a class of Pro/Hyp-rich glycoproteins and are some of the most complex types of macromolecules found in plants. In the economically important plant species, Brassica rapa, only chimeric AGPs have been identified to date. This has significantly limited our understanding of the functional roles of AGPs in this plant. In this study, 64 AGPs were identified in the genome of B. rapa, including 33 classical AGPs, 28 AG peptides and three lys-rich AGPs. Syntenic gene analysis between B. rapa and A. thaliana suggested that the whole genome triplication event dominated the expansion of the AGP gene family in B. rapa. This resulted in a high retained proportion of the AGP family in the B. rapa genome, especially in the least fractionated subgenome. Phylogenetic and motif analysis classified the classical AGPs into six clades and three orphan genes, and the AG peptides into three clades and five orphan genes. Classical AGPs has a faster rate of molecular evolution than AG peptides revealed by estimation of molecular evolution rates. However, no significant differences were observed between classical AGPs and lys-rich AGPs. Under control conditions and in response to phytohormones treatment, a complete expression profiling experiment has identified five anther-specific AGPs and quite a number of AGPs responding to abscisic acid, methyl jasmonate and/or gibberellin. In this study, we presented a bioinformatics approach to identify important types of AGPs. Moreover, the association between their function and their protein structure, as well as the evolution and the expression of AGP genes were investigated, which might provide fundamental information for revealing the roles of AGPs in B. rapa.

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“…The coding gene air12 (for auxin induced in root cultures) is early expressed during auxin-induced lateral root formation in Arabidopsis (Laskowski et al 2002). CIL1, a Brassica carinata ortholog of A. thaliana AIR12, has been shown to be involved in lateral root development and has been suggested to mediate hormone-regulated ROS production (Gibson et al 2012). It has been reported recently that an air12 mutant line shows higher germination rate and reduced growth of lateral roots under abiotic stresses (Gibson and Todd 2015).…”

Section: Introductionmentioning

confidence: 99%

Role of the NAD(P)H quinone oxidoreductase NQR and the cytochrome b AIR12 in controlling superoxide generation at the plasma membrane

Biniek

Heyno

Kruk

et al. 2016

Planta

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The quinone reductase NQR and the b-type cytochrome AIR12 of the plasma membrane are important for the control of reactive oxygen species in the apoplast. AIR12 and NQR are two proteins attached to the plant plasma membrane which may be important for generating and controlling levels of reactive oxygen species in the apoplast. AIR12 (Auxin Induced in Root culture) is a single gene of Arabidopsis that codes for a mono-heme cytochrome b. The NADPH quinone oxidoreductase NQR is a two-electron-transferring flavoenzyme that contributes to the generation of O in isolated plasma membranes. A. thaliana double knockout plants of both NQR and AIR12 generated more O and germinated faster than the single mutant affected in AIR12. To test whether NQR and AIR12 are able to interact functionally, recombinant purified proteins were added to plasma membranes isolated from soybean hypocotyls. In vitro NADH-dependent O production at the plasma membrane in the presence of NQR was reduced upon addition of AIR12. Electron donation from semi-reduced menadione to AIR12 was shown to take place. Biochemical analysis showed that purified plasma membrane from soybean hypocotyls or roots contained phylloquinone and menaquinone-4 as redox carriers. This is the first report on the occurrence of menaquinone-4 in eukaryotic photosynthetic organisms. We propose that NQR and AIR12 interact via the quinone, allowing an electron transfer from cytosolic NAD(P)H to apoplastic monodehydroascorbate and control thereby the level of reactive oxygen production and the redox state of the apoplast.

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Brassica carinata CIL1 mediates extracellular ROS production during auxin- and ABA-regulated lateral root development

Abstract: /npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at

Cited by 9 publications

References 93 publications

Arabidopsis AIR12 influences root development

Arabidopsis AIR12 influences root development

Genomic, Molecular Evolution, and Expression Analysis of Genes Encoding Putative Classical AGPs, Lysine-Rich AGPs, and AG Peptides in Brassica rapa

Role of the NAD(P)H quinone oxidoreductase NQR and the cytochrome b AIR12 in controlling superoxide generation at the plasma membrane

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