Two studies were conducted to establish carinata meal as a partial replacement of conventional protein sources. Study I was conducted to determine the nutrient profile, nitrogen-corrected true metabolizable energy (
TME
n
), and amino acid (
AA
) digestibility of 2 groups: low glucosinolate carinata meal (
LGCM
) and high glucosinolate carinata meal (
HGCM
) using rooster assays. The LGCM contained 28 μmol/g glucosinolate, 11.5% moisture, 39.2% crude protein (
CP
), whereas the HGCM had 100 μmol/g glucosinolate, 10.1% moisture, 39.5% CP on as is basis. The precision-fed rooster assays were conducted to determine TME
n
and AA digestibility. The TME
n
levels of LGCM and HGCM were 1,814 and 1,690 kcal/kg on as is basis, respectively. Standardized digestibility for lysine, methionine, cysteine, threonine, and valine were 72, 88, 69, 75, and 79% for LGCM and 80, 89, 71, 76, and 80% for HGCM, respectively. Based on the nutrient profiles from study I, study II was conducted to evaluate the effects of LGCM and HGCM in broilers. A total of 504 one-day-old Cobb500 male broiler chickens were randomly divided into 42 battery cages with 6 replicates of 12 birds per cage. The seven dietary treatments were control diet, 3 inclusion levels of LGCM (4, 8, and 12%), and 3 of HGCM (4, 8, and12%) in a corn-SBM based diet fed for 21 d. No significant differences in BW, BWG, and FI were observed except for significantly lower BWG in 12% HGCM group compared to control for 14-21 days (
P
< 0.05). The FCR for 12% HGCM increased significantly compared to 4 and 8% of both LGCM and HGCM groups during wk 3 (14–21 d). Based on these studies, carinata meal could be recommended to partially replace conventional feed ingredients at a rate of 12% when LGCM is used and 8% when HGCM is used with no deleterious effects on growth performance, gut histology, and tight junction proteins.