Brassica napus possesses enhanced antioxidant capacity via heterologous expression of anthocyanin pathway gene transcription factors

Nie,; Zhao, Zhiping; Chen, G. P.; Zhang, B.; Ye, M.; Hu, Zongli

doi:10.1134/s1021443713010044

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…Anthocyanins are one of the largest and most important groups of water-soluble pigments in most fruits. Berries, as colored fruits, are highly chemoprotective because of their bioactive anthocyanins [22,23]. Different anthocyanins offer different antioxidant capacity.…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells

et al. 2014

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Blueberry fruits have a wide range of health benefits because of their abundant anthocyanins, which are natural antioxidants. The purpose of this study was to investigate the inhibitory effect of blueberry's two main anthocyanins (malvidin-3-glucoside and malvidin-3-galactoside) on inflammatory response in endothelial cells. These two malvidin glycosides could inhibit tumor necrosis factor-alpha (TNF-α) induced increases of monocyte chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) production both in the protein and mRNA levels in a concentration-dependent manner. Mv-3-glc at the concentration of 1 μM could inhibit 35.9% increased MCP-1, 54.4% ICAM-1, and 44.7% VCAM-1 protein in supernatant, as well as 9.88% MCP-1 and 48.6% ICAM-1 mRNA expression (p < 0.05). In addition, they could decrease IκBα degradation (Mv-3-glc, Mv-3-gal, and their mixture at the concentration of 50 μM had the inhibition rate of 84.8%, 75.3%, and 43.2%, respectively, p < 0.01) and block the nuclear translocation of p65, which suggested their anti-inflammation mechanism was mediated by the nuclear factor-kappa B (NF-κB) OPEN ACCESSMolecules 2014, 19 12828 pathway. In general malvidin-3-glucoside had better anti-inflammatory effect than malvidin-3-galactoside. These results indicated that blueberry is good resource of anti-inflammatory anthocyanins, which can be promising molecules for the development of nutraceuticals to prevent chronic inflammation in many diseases.

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Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells

et al. 2014

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“…Anthocyanins from fruits and vegetables were reported to have anti-inflammatory, antioxidant, anti-cancer, and anti-proliferative properties (Aqil et al, 2012;Nie. et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Colored fruits, particularly berries, are highly chemoprotective because anthocyanins exhibiting potential antioxidant, anti-cancer, anti-proliferative, and anti-inflammatory activities (Aqil et al, 2012;Nie. et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of Malvidin on TNF-α-induced inflammatory response in endothelial cells

Huang

Wang

Liu

et al. 2014

European Journal of Pharmacology

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Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

et al. 2020

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It is indicated that malvidin has anti‐inflammatory and antioxidant effects on various cells, although the function of malvidin in preventing inflammatory reactions caused by lipopolysaccharide (LPS) in peripheral blood mononuclear cells (PBMCs) is still not known. The objective of this study was to examine the impact of malvidin on inflammatory responses and oxidative stress in PBMCs as caused by LPS. The present findings showed that LPS significantly increased the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA and protein release from PBMCs 22 hr after treatments. It was also revealed that increased levels in cytokine expression coincided with increased phosphorylation of JNK, P65‐NF‐κB, and IKKα/IKKβ. Also, the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA induced by LPS as well as secretion of protein in PBMC has been significantly decreased by pretreatment of malvidin. Importantly, pretreatment of the cells with malvidin completely abrogated the phosphorylation of P65‐NF‐κB, JNK, and IKKα/IKKβ in LPS treated cells. Malvidin protection against LPS‐induced inflammation was coupled with a decline in the levels of nitric oxide metabolite and malondialdehyde, along with an increase in the ferric reducing antioxidant power, total thiol activity, and also superoxide dismutase and glutathione peroxidase activity. In accordance with this finding, malvidin may represent a promising therapeutic agent for the prevention of inflammation in PBMCs.

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Brassica napus possesses enhanced antioxidant capacity via heterologous expression of anthocyanin pathway gene transcription factors

Cited by 6 publications

References 27 publications

Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells

Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells

Inhibitory effect of Malvidin on TNF-α-induced inflammatory response in endothelial cells

Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

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