Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs)

Song, Keming; Osborn, T. C.; Williams, Paul H.

doi:10.1007/bf00265606

Cited by 460 publications

(166 citation statements)

References 25 publications

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“…The first work by Song et al [6], based on twelve nuclear and one chloroplastic RFLP probes, suggested that there were two main lineages, one including B. oleracea, B. rapa and the Raphanus genus, the other with B. nigra and the Sinapis genus. This finding was confirmed by Warwick and Black [7] by restriction maps of cp-DNAs.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics to establish genetic relationships in the Brassicaceae family

et al. 2001

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The comparisons of the same proteome (aerial part of etiolated seedlings) in different species and genus of the plant family Brassicaceae (cabbages, mustards, rapes, radishes and Arabidopsis) was undertaken to establish genetic proximities between them. Genetic distances were calculated on the basis of common and distinct spots. The construction of phenetic trees and factorial correspondence analysis that were performed are in very good agreement with our actual knowledge of the taxonomy of the Brassicaceae. The position of the tetraploids, for instance, is in between the positions of their constitutive genome donor representatives. Comparative proteomics may be helpful to shorten the transfer between model and agronomic target species.

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Section: Introductionmentioning

confidence: 99%

Comparative proteomics to establish genetic relationships in the Brassicaceae family

et al. 2001

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“…Analysis of restriction fragment length polymorphisms (RFLPs), especially nuclear RFLPs, provides a useful tool to detect genetic variation among closely related populations and to establish phylogenetic relationships (Figdore et al, 1988;Song et al, 1988aSong et al, , 1988bSong et al, , 1990. A few accessions of Portuguese coles were studied using nuclear RFLPs (Song et al, 1988b(Song et al, , 1990, and the preliminary results suggested that Portuguese tronchuda cabbage and kale may have evolutionary positions between common cabbage and primitive kale.…”

Section: Introductionmentioning

confidence: 99%

Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs

et al. 1991

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RFLPs were used to study the genetic relationships of 31 Portuguese coles (Brassica oleracea L.) representing the different ecotypes or landraces grown in Portugal. Other cole crops such as Jersey kale, Chinese kales, common cabbages, broccolis and cauliflower, two nine-chromosome wild species, and one accession of turnip also were included to determine the evolutionary relationships of Portuguese coles to other related coles. Principal coordinates and cluster analyses were conducted using a package of computer programs and RFLP data from 55 nuclear DNA probes, detecting 291 polymorphic restriction fragments. The results showed that the 48 accessions clustered into five groups: (a) a dispersed group with turnip and the wild brassicas; (b) Chinese kales; (c) broccolis and cauliflower; (d) a disperse group including Algarve and Jersey kales, thousand head kale, cabbages, and savoy cabbage; (e) a large and compact group containing all Portuguese tronchuda cabbages and kales. This unique and closely related group containing Portuguese tronchuda cabbages and kales could be further divided into five subgroups corresponding to the major areas of cultivation. The phenetic groupings of Portuguese tronchuda cabbages and kales based on RFLP data corresponded more with their geographic origins (collecting sites) than with their morphological similarities. A mechanism involving geographic isolation and frequent intercrossing within local areas is proposed to explain genetic relationships among Portuguese tronchuda cabbages and kales.

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“…A search in the EMBL databank did not reveal homology with any other known sequence. The high homology with B. campestris and B. oleracea is supportive of the recent molecular taxonomic classification of Brassica species based on RFLPs [9].…”

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confidence: 55%

A repetitive sequence from Diplotaxis erucoides is highly homologous to that of Brassica campestris and B. oleracea

Harbinder

Lakshmikumaran

1990

Plant Mol Biol

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A tandemly repeated DNA family of Diplotaxis erucoides, a wild relative of Brassica species, has been identified. This repetitive family consists of tandem arrays of a 176 bp repeat unit. This family of highly repeated DNA sequences is also present in many other crucifers [6]. The basic monomeric unit of the tandem repeat of crucifers is 175-180 bp in length except in Sisyumbrium irio [4] and B. nigra [5]. This report deals with the isolation and sequencing of the 176 bp tandem repeated DNA sequences of D. erucoides.DNA from D. erucoides was partially digested with Hind III and ligated to pUC-19 and M13mpl9 vectors. The single-stranded DNA obtained from the recombinant phages was sequenced [7] using 35S-dATP on wedge-shaped 4~ polyacrylamide gels.The T-track reaction indicated the presence of this repeat in 7 out of 12 clones. The complete sequence of three of the repeats and their consensus sequence, showing the different restriction sites is shown in Fig. 1. The repeat has a G + C content of 39~o. The D. erucoides repeat shows 96 ~o homology to B. campestris [7], 94~o to B. oleracea [ 1], 7 2~ to Sinapis alba [2] and 70~o to Raphanus sativus [3] while its homology with S. irio [4] and B. nigra [5] is less than 40~o. These comparisons were done using the algorithm of Wilbur and Lipman [10]. A search in the EMBL databank did not reveal homology with any other known sequence. The high homology with B. campestris and B. oleracea is supportive of the recent molecular taxonomic classification of Brassica species based on RFLPs [9].The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number X16586.

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Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs)

Cited by 460 publications

References 25 publications

Comparative proteomics to establish genetic relationships in the Brassicaceae family

Comparative proteomics to establish genetic relationships in the Brassicaceae family

Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs

A repetitive sequence from Diplotaxis erucoides is highly homologous to that of Brassica campestris and B. oleracea

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