Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater

Fang, Mingxu; Zhang, Wenwu; Zhang, Yanzhou; Tan, Hai-Qin; Zhang, Xin-Qi; Wu, Min; Zhu, Xuemei

doi:10.1099/ijs.0.034660-0

Cited by 58 publications

(37 citation statements)

References 25 publications

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“…Respiratory quinones were detected following the method of Fang et al (2012). The respiratory quinones were Q-8 (95.3 %) and Q-9 (3.6 %), which were similar to those of the species P. xiamenensis, P. arabiensis and P. lipolytica (Zhao et al, 2014;Matsuyama et al, 2013;Xu et al, 2010).…”

Section: The Genome Of Rzy34mentioning

confidence: 80%

Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond

Tian

Wang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-negative, motile, oxidase-and catalase-positive, non-spore-forming, non-pigmented, aerobic bacterium, designated rzy34 T , was isolated from the sediment of a pond containing farmed Fenneropenaeus chinensis Rizhao, China. The strain was able to grow at pH 6-10 (optimum pH 7), 20-40 C (optimum 30 C) and in the presence of 1.0-6.0 % NaCl (optimum 1.0-2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the nearest relative of strain rzy34T was Pseudoalteromonas xiamenensis Y2

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Section: The Genome Of Rzy34mentioning

confidence: 80%

Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond

Tian

Wang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…The polar lipids were extracted, separated on silica gel 60 F 254 plates (10 by 10 cm; Merck), and further analyzed as described previously (52,53). Isoprenoid quinones were analyzed using reversed-phase high-performance liquid chromatography (HPLC) as described previously (54).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci

Sun

Zhang

et al. 2016

Appl Environ Microbiol

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eThe members of the phylum Bacteroidetes are recognized as some of the most important specialists for the degradation of polysaccharides. However, in contrast to research on Bacteroidetes in the human gut, research on polysaccharide degradation by marine Bacteroidetes is still rare. The genus Algibacter belongs to the Flavobacteriaceae family of the Bacteroidetes, and most species in this genus are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae. In this work, a novel brown-seaweed-degrading strain designated HZ22 was isolated from the surface of a brown seaweed (Laminaria japonica). On the basis of its physiological, chemotaxonomic, and genotypic characteristics, it is proposed that strain HZ22 represents a novel species in the genus Algibacter with the proposed name Algibacter alginolytica sp. nov. The genome of strain HZ22, the type strain of this species, harbors 3,371 coding sequences (CDSs) and 255 carbohydrate-active enzymes (CAZymes), including 104 glycoside hydrolases (GHs) and 18 polysaccharide lyases (PLs); this appears to be the highest proportion of CAZymes (ϳ7.5%) among the reported strains in the class Flavobacteria. Seventeen polysaccharide utilization loci (PUL) are predicted to be specific for marine polysaccharides, especially algal polysaccharides from red, green, and brown seaweeds. In particular, PUL N is predicted to be specific for alginate. Taking these findings together with the results of assays of crude alginate lyases, we prove that strain HZ22 T can completely degrade alginate. This work reveals that strain HZ22 T has good potential for the degradation of algal polysaccharides and that the structure and related mechanism of PUL in strain HZ22T are worth further research. Members of the phylum Bacteroidetes, formerly also known as the Cytophaga-Flavobacteria-Bacteroides cluster, constitute one of the major groups of marine heterotrophic bacterioplankton (1, 2). They have been found in various marine habitats, including coastal sediments (3), coastal waters (4, 5), hydrothermal vents (6, 7), and open ocean waters (8-10). In previous studies, marine Bacteroidetes have been reported as important contributors to the utilization of biopolymers such as polysaccharides and proteins (2,(11)(12)(13)(14). As a result, marine Bacteroidetes are assumed to play an important role in the degradation of algae. Marine phytoplankton have been estimated to be responsible for about 50% of global net primary production (15). Polysaccharides constitute a substantial fraction of the primary production from marine phytoplankton. Algae can be an important source of polysaccharides. Brown seaweeds, a traditional and plentiful mariculture product in East Asia, make up a large proportion of the total biomass of algae and synthesize a wide variety of compounds, such as alginate, fucoidan, laminarin, and mannitol (16). Among these compounds, alginate has been assumed to be a potential source for bioethanol production (17-19).The genus Algibacter belongs...

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“…Concentrated sulfuric acid and 5 % ethanolic molybdatophosphoric acid were used to reveal total lipids, ninhydrin for aminolipids, a-naphthol for glycolipids and Zinzadze's reagent for phospholipids (Fang et al, 2012). The results suggested that the major polar lipids of strain NH-JN4 T were diphosphatidylglycerol, phosphatidylglycerol and a diversity of glycolipids (Fig.…”

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confidence: 98%

Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment

Zhang

Fang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China A novel anaerobic bacterium, designated NH-JN4T was isolated from a sediment sample collected in the South China Sea. Cells were Gram-stain-positive, spore-forming, peritrichous and rod-shaped (0.5-1.2¾2.2-7 mm). The temperature and pH ranges for growth were 22-42 6C and pH 6.0-8.5. Optimal growth occurred at 34-38 6C and pH 6.5-7.0. The NaCl concentration range for growth was 0.5-6 % (w/v) with an optimum of 2.5 %. Catalase and oxidase were not produced. Substrates which could be utilized were peptone, tryptone, yeast extract, beef extract and glycine. Main fermentation products from PYG medium were formate, acetate, butyrate and ethanol. Strain NH-JN4 T could utilize sodium sulfite as an electron acceptor.No respiratory quinone was detected. The predominant fatty acids were anteiso-C 15 : 0 , C 16 : 0 , iso-C 15 : 0 , anteiso-C 17 : 0 and C 16 : 0 DMA. (Jung et al., 2010) and the species were divided into 19 cluster groups according to their 16S rRNA gene sequences by Collins et al. (1994). Some species of this genus were isolated from sediments (Jung et al., 2010;Kim et al., 2006Kim et al., , 2007Lee et al., 2007). Here, we describe an obligatory anaerobic, spore-forming and Gram-stainpositive strain, which represents a novel species of a new genus belonging to the family Clostridiaceae.Strain NH-JN4 T was isolated with CJW medium from a sediment sample of the South China Sea (118 u 679 E 21u 959 N), collected by the Second Institute of Oceanography, State Oceanic Administration of China in 2010. CJW medium contained (l 21 distilled water) 1.0 g Na 2 SO 4 , 30.0 g NaCl, 5.0 g MgCl 2 . 6H 2 O, 0.7 g KCl, 1.0 g CaCl 2 . 2H 2 O, 0.5 g NH 4 Cl, 0.4 g K 2 HPO 4 , 0.4 g KH 2 PO 4 , 2.0 g NaHCO 3 , 2.0 mg Fe(NH 4 ) 2 (SO 4 ) 2 , 3.0 g yeast extract (Difco), 4.0 g tryptone (Difco), 0.6 g Lcysteine, 0.001 g resazurin, pH 6.8. To make PYG medium 10.0 g glucose, 7.0 g yeast extract, 1.0 g tryptone and 5.0 g peptone were added to CJW medium. To make solid medium, 1.5 % agar was added. Sediment was dispersed and suspended in sterile CJW medium. The clear liquid of the upper layer was inoculated onto plates anaerobically and cultivated at 37 u C in the anaerobic chamber (Bugbox; Ruskinn) until colonies appeared. The Hungate roll-tube technique (Hungate, 1969;Bryant, 1972) was used to Abbreviation: FAME, fatty acid methyl ester.

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Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater

Cited by 58 publications

References 25 publications

Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond

Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci

Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment

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