Brazilian Propolis: physicochemical properties, plant origin and antibacterial activity on periodontopathogens

Abstract

Propolis samples collected in the dry and rainy seasons, from an experimental apiary located in a cerrado vegetation region in Brazil were used in this study. Microscopic analysis showed the presence of 31 pollen types, secretory hairs (genus Baccharis) and fragments of plant epidermis. The oxidation rates and the wax content of the samples after physicochemical analyses were in agreement with the Cuban Guideline NRAG 870-88. A high performance liquid chromatography analysis showed a similar pattern of chromatograms, characterized by the presence of ten phenolic compounds. There was no significant difference in the pro fi le of phenolic compounds and also in the total flavonoid concentration in propolis samples collected in different seasons. Antibacterial assays were performed by the method of dilution of an ethanol extract of propolis (EEP) in agar (v/v%) and showed that all 16 A. actinomycetemcomitans strains tested were inhibited by propolis concentrations of 0.1% to 0.25%, and did not grow at all at 0.5%. The growth inhibition of six Fusobacterium spp. and 16 black-pigmented anaerobes was observed at concentrations of 0.05% to 0.1%, and no growth was observed at 0.25%. There was no effect of seasonality on the inhibitory activity of propolis. The antibiotics tetracycline and meropenem were used as positive controls.

Pollen types and levels of total phenolic compounds in propolis produced by Apis mellifera L. (Apidae) in an area of the Semiarid Region of Bahia, Brazil

“…This fact led some authors to consider the species of this genus from the family Myrtaceae as one of the possible sources of Brazilian propolis (Bastos et al 2000, Santos et al 2003, Freitas et al 2010. Eucalyptus L'Hér.…”

Perfis físico-químico e cromatográfico de amostras de própolis produzidas nas microrregiões de Franca (SP) e Passos (MG), Brasil

“…Várias substâncias presentes na própolis provêm de fl ores, ramos, brotos, exsudatos e de outras partes do tecido vegetal. Estas substâncias podem ser modifi cadas na colméia pela adição de secreções salivares (Santos et al, 2003). Estudos realizados mostram que mais de trezentas substâncias foram identifi cadas em amostras de própolis, com predominância de fl avonóides (fl avonas, fl avonóis, fl avanonas), das quais destacam-se: galangina, crisina, tectocrisina, pinocembrina, campferol e quercetina, bem como os aldeídos aromáticos (vanilina e isovanilina), cumarinas, ácidos fenólicos (ácido caféico, ferúlico, cinâmico e cumárico), ácidos orgânicos (ácido benzóico) e alguns oligoelementos, tais como: alumínio, vanádio, ferro, cálcio, silício, manganês, estrôncio, e vitaminas B1, B2, B6, e C (Bankova et al, 1998;Bankova et al, 2000;Marcucci et al, 2001).…”

Possible Cardioprotective Action of Pomegranate Juice Punica Granatum and Propolis Against Myocardial Infarction Induced in Rats.

“…Propolis considered as anti-tumor, anti-bacterial, anti-fungal, anti-viral, anti-inflammatory anti-oxidant, anti-cancer and immune-stimulant (Santos et al, 2003;Banskota et al, 2001;Valente et al,2011;Sforcin 2007). The aim of current to evaluate the protective effect of propolis extract, PJ or a combined with propolis extract against incidence of MI in rats.…”