Brazilian red propolis exhibits antiparasitic properties in vitro and reduces worm burden and egg production in an mouse model harboring either early or chronic Schistosoma mansoni infection

Silva, Marcos P.; Silva, Thiago M.; Mengarda, Ana C.; Salvadori, M. C.; Teixeira, F. S.; Alencar, Severino Matias de; Filho, Givelton C. Luz; Bueno‐Silva, Bruno; Moraes, Josué de

doi:10.1016/j.jep.2020.113387

Cited by 36 publications

(27 citation statements)

References 53 publications

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“…Other factors reported to influence the dissimilarity of the physicochemical characteristics of the bee products are the vegetation surrounding the beehive, collection time, soil diversity, geoclimatic conditions or seasons in the collection area, the bee species, and particular flora living at the harvesting location [226][227][228]. Variations in the concentration of effective bee products are also predominantly affected by the type and origins of parasites used in the experiments as well as the preparation method [198,229,230]. There is a wide range of the extraction method applied to obtain, for example, propolis extracts ranging from conventional separation technique using organic solvent such as ethanol to a more sophisticated one such as a supercritical fluid extraction method [231].…”

Section: Antiparasitic Potential Of Bee Productsmentioning

confidence: 99%

Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

et al. 2021

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Bee products have long been used in traditional healing practices to treat many types of disorders, including cancer and microbial-related diseases. Indeed, several chemical compounds found in bee products have been demonstrated to display anticancer, antibacterial, antiviral, and antiparasitic properties. With the improvement of research tools and in view of recent advances related to bee products, this review aims to provide broad yet detailed insight into the pharmaceutical prospects of bee products such as honey, propolis, bee pollen, royal jelly, bee bread, beeswax, and bee venom, in the domain of cancer and infectious disease management. Available literature confirms the efficacy of these bee products in the alleviation of cancer progression, inhibition of bacterial and viral proliferation, and mitigation of parasitic-related symptoms. With such potentials, bioactive components isolated from the bee products can be used as an alternative approach in the long-run effort to improve humans’ health at a personal and community level.

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Section: Antiparasitic Potential Of Bee Productsmentioning

confidence: 99%

Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

et al. 2021

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“…Thus, our data suggest that amiodarone should have antischistosomal properties under its current antiarrhythmic drug regimen. Although the amiodarone is commonly used in clinical practice, the risk of adverse effects acts as a limiting factor to its utilization [34][35][36][37][38][39][40][41][42][43][44][45][46][47]. As community-wide treatment would be expected at least in most endemic areas for schistosomiasis, this would be a problem.…”

Section: Discussionmentioning

confidence: 99%

“…For in vivo efficacy studies, mice were each infected subcutaneously with 80 S. mansoni cercariae. Animals were then randomly divided into experimental groups (five mice per group), and amiodarone was tested using a single oral dose (400 mg/kg) or once daily for 5 consecutive days (100 mg/kg/day) 21 days post-infection (immature stage, early infection) or 49 days post-infection (adult stage, chronic infection) [ 15 , 41 ]. For comparison, praziquantel was administered at 400 mg/kg to groups of five S. mansoni -infected animals in the same period.…”

Section: Methodsmentioning

confidence: 99%

Antiparasitic Properties of Cardiovascular Agents against Human Intravascular Parasite Schistosoma mansoni

et al. 2021

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The intravascular parasitic worm Schistosoma mansoni is a causative agent of schistosomiasis, a disease of great global public health significance. Praziquantel is the only drug available to treat schistosomiasis and there is an urgent demand for new anthelmintic agents. Adopting a phenotypic drug screening strategy, here, we evaluated the antiparasitic properties of 46 commercially available cardiovascular drugs against S. mansoni. From these screenings, we found that amiodarone, telmisartan, propafenone, methyldopa, and doxazosin affected the viability of schistosomes in vitro, with effective concentrations of 50% (EC50) and 90% (EC90) values ranging from 8 to 50 µM. These results were further supported by scanning electron microscopy analysis. Subsequently, the most effective drug (amiodarone) was further tested in a murine model of schistosomiasis for both early and chronic S. mansoni infections using a single oral dose of 400 mg/kg or 100 mg/kg daily for five consecutive days. Amiodarone had a low efficacy in chronic infection, with the worm and egg burden reduction ranging from 10 to 30%. In contrast, amiodarone caused a significant reduction in worm and egg burden in early infection (>50%). Comparatively, treatment with amiodarone is more effective in early infection than praziquantel, demonstrating the potential role of this cardiovascular drug as an antischistosomal agent.

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“…Brazilian red propolis demonstrated strong cytotoxic potential in vitro , suggesting a potential therapeutic alternative for treatment against Chagas disease ( Dantas Silva et al, 2017 ), schistosomiasis ( Silva et al, 2021 ), and some types of cancer ( da Silva Frozza et al, 2013 ; Dantas Silva et al, 2017 ; Banzato et al, 2020 ). These properties are related to propolis complex chemical composition, including formononetin, isoliquiritigenin, liquiritigenin, and biochanin A, as described by da Silva Frozza et al (2013) .…”

Section: Introductionmentioning

confidence: 99%

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

et al. 2021

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Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

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Brazilian red propolis exhibits antiparasitic properties in vitro and reduces worm burden and egg production in an mouse model harboring either early or chronic Schistosoma mansoni infection

Cited by 36 publications

References 53 publications

Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

Antiparasitic Properties of Cardiovascular Agents against Human Intravascular Parasite Schistosoma mansoni

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

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