BRCA1 is localized in ductal secretions of normal breast tissues: Detection by antibodies raised against the amino terminus of BRCA1 (K-18)

Bernard‐Gallon, Dominique; Latour, Monique Peffault De; Oliveira, Frédéric De; Rio, Pascale; Hızel, Candan; Favy, D; Bignon, Yves‐Jean

doi:10.1002/(sici)1097-0215(19980831)77:5<803::aid-ijc21>3.0.co;2-0

Cited by 10 publications

(4 citation statements)

References 8 publications

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“…2,3 BRCA1 has been variously localised to the nucleus of normal breast epithelial cells, 4 ± 8 to cylinders derived from cytoplasmic structures which anastamose through the nucleus 9 and to the secretory apparatus 10 and associated secretions. 11,12 In addition, it has been reported to both remain in the nucleus 7 and to be present in the cytoplasm of breast cancer cell 4 ± 6 (reviewed in 13,14 ). Some or all of these observations may be reconciled by the hypothesis that BRCA1 transcripts are differentially spliced, 15 ± 17 and that the different mature proteins are directed to specific subcellular sites.…”

Section: Introductionmentioning

confidence: 99%

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

O'Connell

Martin

2000

Cell Death Differ

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Brca1 mRNA was detectable in female mouse mammary gland tissue from adult virgins, during pregnancy and early lactation. It was associated with phases of mammary epithelial cell proliferation and differentiation but the pattern of Brca1 expression was dissociable from that of a true differentiation marker, b-casein, by virtue of its significant expression in the virgin gland and termination of its expression in early lactation. In a primary cell culture model, association of a laminin-rich extracellular matrix (ECM) with mammary epithelial cells was required for cell survival and cell differentiation and suppressed Brca1 expression in these cells. ECM-association may significantly contribute to the final restriction in Brca1 expression in the lactating gland in vivo. Interestingly, our results suggest that mammary epithelial cells undergo apoptosis both when expressing and when not expressing Brca1, depending on whether the dying cell populations had been terminally differentiated or not. Cell Death and Differentiation (2000) 7, 360 ± 367.

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Section: Introductionmentioning

confidence: 99%

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

O'Connell

Martin

2000

Cell Death Differ

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“…The specificity of the polyclonal antibody against BRCA1 (K-18) has been demonstrated elsewhere for BRCA1 [6,7], ascertained by Western blotting, and the 220 kDa band corresponding to BRCA1 was detected in both HBL-100 and MCF-7 breast cell lines. Moreover, other major bands appeared around 100 kDa in the two cell lines, which may correspond to different variants to BRCA1 [7,8].…”

Section: Resultsmentioning

confidence: 95%

“…Moreover, other major bands appeared around 100 kDa in the two cell lines, which may correspond to different variants to BRCA1 [7,8]. …”

Section: Resultsmentioning

confidence: 99%

BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

Bernard‐Gallon

Déchelotte²,

Vissac

et al. 2000

Breast Cancer Res

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BRCA1 and BRCA2 breast cancer susceptibility genes encode proteins, the normal cellular functions of which are complex and multiple, and germ-line mutations in individuals predispose both to breast and to ovarian cancer. There is nevertheless substantial evidence linking BRCA1 and BRCA2 to homologous recombination and DNA repair, to transcriptional control and to tissue proliferation. There is controversy regarding the localization of BRCA1 and BRCA2 proteins to either nucleus or cytoplasm and whether the expression is present in premeiotic germ cells or can still be expressed in mitotic spermatogonia. We report herein an immunohistochemical study of BRCA1 and BRCA2 distribution in a rather unsual tissue (an ovotestis), which addresses this issue.

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“…A patient suffering from apocrine metaplasia also presented apical cytoplasmic staining and apocrine secretion staining in the lumen of the tubes with K-18 antibodies (Bernard-Gallon et al, 1998). Coene et al (1997) reported localization of BRCA1 in the perinuclear compartment of the endoplasmic reticulum-Golgi complex and in tubes invaginating the nucleus in breast tissues.…”

Section: Brca1 and Brca2 Proteins Are Expressed In Milk Fat Globulesmentioning

confidence: 99%

BRCA1 and BRCA2 proteins are expressed in milk fat globules

et al. 1999

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BRCA1 is localized in ductal secretions of normal breast tissues: Detection by antibodies raised against the amino terminus of BRCA1 (K-18)

Cited by 10 publications

References 8 publications

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

BRCA1 and BRCA2 proteins are expressed in milk fat globules

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