Breaking seed dormancy of Astragalus penduliflorus Lam.

Abstract

<em>Astragalus penduliflorus</em> Lam. is an alpine-subalpine species. Several isolated populations occur in Europe: in the Alps, Pyrenees, Carpathians and in central Sweden. <em>Astragalus penduliflorus</em> is considered as critically endangered species in Poland, growing only at the locality in the Smytnia Valley, in the Western Tatra Mountains. The population is at risk, due to the limited reproduction caused by law rate of seed germination, periodically shortened vegetation period that prevent seed development and gnawing the aerial plant parts by deer. The aim of the study was to explain the reason for the poor germination of <em>A. penduliflorus</em> seeds. As a result of mechanical scarification, 100% of <em>A. penduliflorus</em> seeds germinated, which proved that these seeds are characterized by a water-impermeable seed coat, which classified them as hard seeds that go through physical dormancy. Results obtained in this work can be used for effective reproduction and active conservation of threatened <em>A. penduliflorus</em>.

In Vitro Culture as a Tool for Studying Plant Developmental Processes at the Physiological Level in Poland

“…e populations of many plant species are endangered owing to the limited potential of generative reproduction and low rate of seed germination. Previously, the positive effect of scarification on A. penduliflorus seed germination was demonstrated, thereby confirming that the seeds show physical dormancy owing to the impermeable seed coat (K. Dziurka, Skrzypek, & Dubert, 2019). Breaking the seed dormancy of A. penduliflorus increased the seed germination rate up to 100%; however, nearly half of the seedlings were infected.…”

“…Breaking the seed dormancy of A. penduliflorus increased the seed germination rate up to 100%; however, nearly half of the seedlings were infected. erefore, to increase the number of healthy seedlings obtained, seeds should be disinfected, mechanically scarified, and germinated under in vitro conditions (K. Dziurka, Skrzypek, & Dubert, 2019). Healthy seedlings of A. penduliflorus were used as the starting material for in vitro culture.…”

Application of Horticultural and Tissue Culture Methods for Ex Situ Conservation of Endangered Primula farinosa L.

“…Seed germination behavior is an integral part of ex situ conservation, and understanding the germination requirements is the first step in the reinforcement of endangered plant species [e.g., Cerabolini et al, (2004); Cho et al, (2018); Dziurka et al, (2019)]. The ability of P. farinosa seeds to germinate, assessed in the germination test in consecutive years, was low, despite high (over 90%) seed viability confirmed in our previous work using tetrazolium (Gajewski et al, 2013).…”

Bazı Dormansi Kırma Uygulamalarının ve Sıcaklığın Sakız Geveni’nin (Astragalus gummifer Labill.) Tohum Canlılığı Üzerine Etkileri

“…Mechanical scarification has been applied to seeds of many Astragalus species. Mechanical scarification increased the germination rate of the seeds compared to the control in Astragalus peckii, Astragalus penduliflorus, Astragalus filipes, Astragalus siliquosus, Astragalus hamosus, Astragalus cicer, Astragalus fridae, Astragalus tribuloides, Astragalus bibullatus, and Astragalus contortuplicatus seeds and determined that mechanical scarification is an effective method for breaking dormancy (Miklas et al, 1987;Isavand et al, 2005;Eisvand et al, 2006;Fateh et al, 2006;Patane and Gresta, 2006;Arbabian et al, 2009;Albrecht et al, 2012;Molnár et al, 2015;Pearson, 2015;Jones et al, 2016;Schnadelbach et al, 2016;Siles et al, 2016;Statwick, 2016;Kildisheva et al, 2018;Dziurka et al, 2019).…”

“…It was determined that the application of sulfuric acid to Astragalus cicer and Astragalus hamosus seeds did not increase the germination rate of the seeds compared to the control, and it was not an effective method for breaking dormancy (Siles et al, 2016;Statwick, 2016). On the other hand, the application of sulfuric acid to the seeds of Astragalus vulnerariae, Astragalus adsurgens, Astragalus penduliflorus, Astragalus maritimus, Astragalus verrucosus, Astragalus adscendens, Astragalus podolobus, Astragalus siliquosus, Astragalus hamosus, Astragalus lehmannianus, Astragalus cyclophyllon, Astragalus armatus, Astragalus sinicus, Astragalus cicer, and Astragalus arpilobus determined that it is an effective method in breaking dormancy (Miklas et al, 1987;Kondo and Takeuchi, 2004;Isavand et al, 2005;Eisvand et al, 2006;Patane and Gresta, 2006;Keshtkar et al, 2008;Kim et al al., 2008;Bacchetta et al, 2011;Long et al, 2012;Abudurehman et al, 2014;Tavili et al, 2014;Dilaver et al, 2017;Kheloufi et al, 2018;Dziurka et al, 2019).…”