Breakthroughs in microbiology made possible with organoids

Ramírez-Flores, Carlos J; Knoll, Laura J.

doi:10.1371/journal.ppat.1010080

Cited by 9 publications

(5 citation statements)

References 34 publications

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“…They have reported the 3D biomimetic liver models can be appropriate model and can found its promising biomedical applications and to study liver steatohepatitis. 33,34 The 3D gastric organoids application could be very helpful and can provide the key conversion from the mitotic spindle to the central spindle before anaphase onset. Also, gastric organoids provide the platform for infection caused by Helicobacter pylori.…”

Section: Application In Tissue Engineeringmentioning

confidence: 99%

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Organoids technology: an advanced meditate in tissue engineering and organ development

Shilpi,

Chimaniya,

Saini

et al. 2023

ATROA

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Organoids are in vitro miniaturized and simplified model systems of organs that have attracted a great deal of interest for cell therapy, personalized medicine, drug screening, and the modelling of tissue development and treatment of different illness. Despite significant progress in the cultivation of physiologically appropriate organoids, obstacles still stand in the way of practical applications. Organoid systems are difficult to translate in part because of the significant diversity of self-organizing growth and the limited experimental and analytical access. Since ancient times, efforts have been attempted repeatedly to create replicas of in vivo organs from their tissues or cells. Organoid technology as a whole has only recently begun to systematically develop and has been demonstrated to be crucial in tissue engineering. Induced and retrieved stem cells from a variety of organs have the ability to self-organize to create three dimensional structures that are physically and functionally equivalent to their in vivo counterparts. These organoid models offer a strong foundation for understanding disease modeling, drug candidate screening, and development mechanisms. In this review it has been describe the developments to produce different organoids of tissues as well as their shortcomings and potential applications in tissue engineering.

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Section: Application In Tissue Engineeringmentioning

confidence: 99%

“…Also, gastric organoids provide the platform for infection caused by Helicobacter pylori. [34][35][36]…”

Section: Application In Tissue Engineeringmentioning

confidence: 99%

Organoids technology: an advanced meditate in tissue engineering and organ development

Shilpi,

Chimaniya,

Saini

et al. 2023

ATROA

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“…1). Mouse and human enteroids have enabled important discoveries in infectious disease research, including studies of Apicomplexan parasites (Heo et al 2018, Wilke et al 2019, Ramírez-Flores & Knoll 2021. While feline enteroids have been used for infectious disease research by our lab and others, they remain difficult to work with because they undergo early growth arrest and cell death (Powell & Behnke 2017, Tekes et al 2020.…”

Section: Introductionmentioning

confidence: 99%

Fibroblasts enhance the growth and survival of adult feline small intestinal organoids

Hryckowian,

Studener,

Knoll

2024

Preprint

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Intestinal organoids are important cell culture models that complement live animal studies of intestinal biology. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth and rapid senescence. We introduce a method of co-culturing adult feline small intestinal organoids with growth inhibited human foreskin fibroblast feeder cells to enhance organoid proliferation and survival. With feeder cells, feline jejunal and ileal organoids survived at least nine months in culture until cryopreservation. Fibroblast supplementation also increased the maximum size of cat and mouse intestinal organoids, making them easier for microinjection. The increased proliferation, longevity, and size make fibroblast-supplementation of feline small intestinal organoids a significant improvement on current methods. These methods have high potential to reduce the number of cats used for research and may be applicable for intestinal cells from other animals that are difficult to culture.

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“…Organoids infected with pathogens are accustomed to perceiving the infection and unwellness progression in human‐like tissues. Recently, “Intelligent” organoids developed by researchers at the University of Bristol were specifically used to demonstrate the interaction with microbes (Ramírez‐Flores & Knoll, 2021). Organoids can be programmed and tuned with artificial sequence circuits that enable the assessment of gene expression upon microorganism infection.…”

Section: Introductionmentioning

confidence: 99%

“…Organoids can be programmed and tuned with artificial sequence circuits that enable the assessment of gene expression upon microorganism infection. The mechanism of interactions between microorganisms and organoid cells has been investigated to resolve varied queries regarding gut microbes' pathophysiology which would help to assess drug efficacy in totally different microbial populations (Fujii et al, 2018; Ramírez‐Flores & Knoll, 2021).…”

Section: Introductionmentioning

confidence: 99%

3D printed human organoids: High throughput system for drug screening and testing in current COVID‐19 pandemic

Parihar

Pandita

Khan

2022

Biotech & Bioengineering

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In the current pandemic, scenario the world is facing a huge shortage of effective drugs and other prophylactic medicine to treat patients which created havoc in several countries with poor resources. With limited demand and supply of effective drugs, researchers rushed to repurpose the existing approved drugs for the treatment of COVID‐19. The process of drug screening and testing is very costly and requires several steps for validation and treatment efficacy evaluation ranging from in‐vitro to in‐vivo setups. After these steps, a clinical trial is mandatory for the evaluation of treatment efficacy and side effects in humans. These processes enhance the overall cost and sometimes the lead molecule show adverse effects in humans and the trial ends up in the final stages. Recently with the advent of three‐dimensional (3D) organoid culture which mimics the human tissue exactly the process of drug screening and testing can be done in a faster and cost‐effective manner. Further 3D organoids prepared from stems cells taken from individuals can be beneficial for personalized drug therapy which could save millions of lives. This review discussed approaches and techniques for the synthesis of 3D‐printed human organoids for drug screening. The key findings of the usage of organoids for personalized medicine for the treatment of COVID‐19 have been discussed. In the end, the key challenges for the wide applicability of human organoids for drug screening with prospects of future orientation have been included.

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Breakthroughs in microbiology made possible with organoids

Cited by 9 publications

References 34 publications

Organoids technology: an advanced meditate in tissue engineering and organ development

Organoids technology: an advanced meditate in tissue engineering and organ development

Fibroblasts enhance the growth and survival of adult feline small intestinal organoids

3D printed human organoids: High throughput system for drug screening and testing in current COVID‐19 pandemic

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