Breast Cancer Cell Cultures on Electrospun Poly(ε-Caprolactone) as a Potential Tool for Preclinical Studies on Anticancer Treatments

Bazzolo, Bianca; Sieni, Elisabetta; Zamuner, Annj; Roso, Martina; Russo, Teresa; Gloria, Antonio; Dettin, Monica; Conconi, Maria Teresa

doi:10.3390/bioengineering8010001

Cited by 15 publications

(12 citation statements)

References 59 publications

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“…Compared to the 2D culture, a decrease in drug sensitivity to doxorubicin and electroporation/bleomycin was also observed in the 3D scaffold model. The possible reason for a reduction in sensitivity might be the abundant CSC population or ECM presence ( 62 ). The synthetic polymers, PCL and PLA, were capable of mimicking the structural properties of native ECM, but they failed in providing the biochemical signals needed for cell-ECM communication.…”

Section: Fibrous Scaffolds As In Vitro Cancer Modelsmentioning

confidence: 99%

Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update

2021

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The lack of traditional cancer treatments has resulted in an increased need for new clinical techniques. Standard two-dimensional (2D) models used to validate drug efficacy and screening have a low in vitro-in vivo translation potential. Recreating the in vivo tumor microenvironment at the three-dimensional (3D) level is essential to resolve these limitations in the 2D culture and improve therapy results. The physical and mechanical environments of 3D culture allow cancer cells to expand in a heterogeneous manner, adopt different phenotypes, gene and protein profiles, and develop metastatic potential and drug resistance similar to human tumors. The current application of 3D scaffold culture systems based on synthetic polymers or selected extracellular matrix components promotes signalling, survival, and cancer cell proliferation. This review will focus on the recent advancement of numerous 3D-based scaffold models for cancer tissue engineering, which will increase the predictive ability of preclinical studies and significantly improve clinical translation.

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Section: Fibrous Scaffolds As In Vitro Cancer Modelsmentioning

confidence: 99%

Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update

2021

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“…Several studies have demonstrated the efficacy of hydrophobic materials such as ethyl cellulose, polycaprolactone (PCL), poly (lactic-co-glycolic acid), and polylactic acid in sustaining drug release ( Sánchez-Romate et al, 2020 ; Ahmadi et al, 2022 ; Wang S. et al, 2022 ; Dodda et al, 2022 ; Lee et al, 2022 ; Vlachopoulos et al, 2022 ). PCL, a hydrophobic material processing good biocompatibility, is extensively involved in the preparation of biomedical materials ( Jiménez-Suárez et al, 2020 ; Bazzolo et al, 2021 ; Sánchez-Romate et al, 2021 ; Baghali et al, 2022 ; Setia Budi et al, 2022 ; Shahverdi et al, 2022 ). In the treatment of osteoporosis, simvastatin (SIM) has been exploited for inducing bone regeneration ( Castro et al, 2018 ; Rezk et al, 2020 ; Karanikola et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Electrospun core–sheath PCL nanofibers loaded with nHA and simvastatin and their potential bone regeneration applications

Qian

Liu

Chen

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Drugs and biocompatible nanoparticles have raised significant potential in advancing the bone regeneration. Electrospinning technology enables the full realization of the value of drugs and nanoparticles.Methods: In this study, we have successfully fabricated core–sheath nanofibers solely composed of polycaprolactone (PCL) polymer. Simvastatin (SIM) was confined to the core of the nanofibers while nanohydroxyapatite (nHA) was loaded on the nanofiber surface.Results: All the prepared nanofibers exhibited a cylindrical micromorphology, and the core–sheath structure was exploited using a Transmission Electron Microscope. X-ray pattern results indicated that SIM was in an amorphous state within nanofibers, while Fourier Transform InfraRed spectroscopy showed excellent chemical compatibility among SIM, nHA, and PCL. The actual loading of nHA within the nanofiber was determined by a thermogravimetric test due to the high melting point of nHA. Core–sheath nanofibers could release SIM for 672 h, which was attributed to the core–sheath structure. Furthermore, nanofibers loaded with SIM or nHA had a positive impact on cell proliferation, with the core–sheath nanofibers displaying the most favorable cell proliferation behavior.Discussion: Such a synergistic facilitation strategy based on materials and nanostructure may encourage researchers to exploit new biomedical materials in future.

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“…Designing and developing pro-angiogenic electrospun PCL nanofibers and evaluating their usefulness in various tissue engineering and regenerative medicine settings is an active field of research [ 24 ]. Bazzolo et al [ 25 ] conducted breast cancer cell cultures on electrospun PCL as a potential tool for preclinical studies on anticancer treatments. Various electrospun nanofibers made of natural and synthetic polymers are often used to incorporate biomolecules and peptides to improve the physico-chemical/mechanical and biological properties of PCL fibers.…”

Section: Introductionmentioning

confidence: 99%

“…The study by Dettin M et al [ 27 ] investigates the influence of increasing concentrations self-assembling peptides (SAP) with PCL on the physico-chemical, mechanical and biological properties of PCL fibers. The study found that, as compared with bare PCL, SAP enrichment increased the number of metabolic active h-osteoblast cells, fostered the expression of specific osteoblast-related mRNA transcripts, and guided calcium deposition, revealing the potential application of PCL-SAP scaffolds for the maintenance of osteoblast phenotype [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Polycaprolactone Electrospun Nanofiber-Composites for Artificial Skin Based on Dermal Fibroblast Culture

et al. 2022

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The study’s aim was to develop a dermal equivalent scaffold that can mimic the architecture and biological performance of the human dermis. Poly ε-caprolactone (PCL) electrospun nanofiber material (ENF) was assembled with polyethylene glycol diacrylate (PEGDA), sodium alginate (SA) and type I collagen (CG1) to develop three groups of dermal equivalent scaffolds. These scaffolds were named PEGDA-PCL, SA-PCL and CG1-PCL. Scanning electron microscopy (SEM) images of cell-free scaffolds’ top and cross-sectional surface were collected and analyzed to examine internal morphology, specifically the adhesiveness of PCL fibers with the different scaffolds. Human dermal fibroblasts were cultured on each of the scaffolds. Cell viability studies including cell adhesion, cell differentiation and stress fiber production were conducted on each scaffold. Furthermore, the architectural integrity of each scaffold was verified by degradation analysis for 2 weeks by soaking each scaffold in phosphate-buffered saline (PBS) solution. Finally, we conducted rheological characteristics of each scaffold. Based on our results from the above analysis, the study concluded that CG1-PCL is best suitable for the dermal equivalent model and has potential to be used as a graft for skin repair.

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Breast Cancer Cell Cultures on Electrospun Poly(ε-Caprolactone) as a Potential Tool for Preclinical Studies on Anticancer Treatments

Cited by 15 publications

References 59 publications

Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update

Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update

Electrospun core–sheath PCL nanofibers loaded with nHA and simvastatin and their potential bone regeneration applications

Evaluation of Polycaprolactone Electrospun Nanofiber-Composites for Artificial Skin Based on Dermal Fibroblast Culture

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