Breeding of a long shelf-life strain for commercial cultivation by mono–mono crossing in Pleurotus eryngii

Factors affecting mushroom Pleurotus spp.

“…Sterilization is an important step for mushroom cultivation. Several studies have reported the use of heat treatment such as sterilization, as reported by Moonmoon et al, 2010, Kim et al, 2013, Mejía and Albertó, 2013 and Ryu et al (2015), wherein the cultivation packages were sterilized in an autoclave for 1, 1.40 and 2 h, respectively, at 121 °C 1.2 psi of pressure. Previous studies tested different sterilization techniques including hot water, autoclave, formalin and bavistin (Hussain et al, 2002).…”

“…According to Ahmed et al (2013), for the cultivation of P. high-king , P. ostreatu s and P. geesteranus , temperature of culture house was maintained at between 22 and 25 °C. Similarly, Kim et al (2013) also cultivated P eryngii , where incubation room temperature was maintained at 22–24 °C. According to Hoa and Wang (2015), the optimal temperature for both P. ostreatus and P. cystidiosus was found to be 28 °C.…”

Development of cultivation media for extending the shelf-life and improving yield of king oyster mushrooms (Pleurotus eryngii)

“…No significant correlation between hardness and shelf life was observed (Philippoussis et al, 2001a), it is consistent with our results. The hardness determined by previous method (Kim et al, 2013a) was no correlation with shelf life grades (data not shown). Thus, a modified method was adapted; texture was determined by measuring a compressibility force using a spherical (ball) probe not penetration (fracture) force using a dia probe.…”

“…Packaging under a passive modified atmosphere was reported to affect the sensory characteristics and shelf life of Lentinula edodes (Ares et al, 2006). Otherwise, breeding for long shelf life was performed in P. eryngii (Kim et al, 2013a). We sought basic solutions for producing mushrooms with a longer shelf life by adjusting the nutritional factors.…”

Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis) for Crossbreeding

“…Crossbreeding plays an important role in mushroom breeding [ 6 , 7 ], but it is seldom done with Bailinggu. Conventional procedures of mushroom crossbreeding include monokaryon isolation and identification from dikaryotic strains, determination of their mating types, cross-mating two monokaryotic mycelia, microscopic observation of clamp connections at the contact line and hybrid offspring detection [ 2 , 6 , 7 , 8 ]. However, these conventional methods are troublesome and time consuming.…”