Development of cultivation media for extending the shelf-life and improving yield of king oyster mushrooms (Pleurotus eryngii)

Ryu, Jae-San; Kim, Min Keun; Im, Chak Han; Shin, Pyung-Gyun

doi:10.1016/j.scienta.2015.07.005

Cited by 37 publications

(27 citation statements)

References 31 publications

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“…According to Bellettini et al [38], among the most important factors are the https://doi.org/10.1007/s42452-020-03720-z substrates used, the carbon-nitrogen ratio (C/N) (which should be within the range of 25-100/1), pH, particle size, and quantity of inoculum. The balance between the C/N ratio in the substrate is important to promote the proper mycelial development of the mushrooms because the total carbon is composed of recalcitrant cellulose and hemicellulose [27]. In a study by Li et al [39], for example, it was observed that the low C/N ratio (excess of nitrogen) in stems of perilla slows the formation of basidiocarps.…”

Section: Discussionmentioning

confidence: 99%

“…Agronomic wastes from rice and corn are important lignocellulosic sources of C and N, with a C/N ratio greater than 30. This allows these materials to be used as substrates in the cultivation of P. ostreatus, owing to its specialization in the decomposition of substrates [9,27]. This study aimed to provide evidence that the most abundant substrates in Peru, corn stubble and rice straw, can also be used in the production of the edible mushroom P. ostreatus, which can provide a valuable food while contributing to the management and recycling of agronomic wastes.…”

Section: Introductionmentioning

confidence: 99%

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Use of lignocellulosic corn and rice wastes as substrates for oyster mushroom (Pleurotus ostreatus Jacq.) cultivation

et al. 2020

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The accumulation of agronomic wastes has caused several environmental problems, such as air and soil contamination, and insect and pathogen proliferation, among others. To mitigate this, studies have evaluated the use of these wastes as substrates for the cultivation of Pleurotus mushrooms, a low-cost/nutritionally important crop. This study aimed to evaluate the use of corn stubble and rice straw as substrates for the in vitro production of oyster mushrooms (Pleurotus ostreatus Jacq.) and the productive characterization (biological efficiency, production rate, earliness, daily productive capacity) of four isolated strains. The strains PO/A01, PO/A02, PO/A03, and PO/A04 were grown in Potato-Dextrose-Agar medium until complete colonization. The experiment was evaluated under a completely randomized design with 6 replications. Subsequently, the corn stubble and rice straw were disinfected, inoculated, bagged, incubated, and subjected to induction-fructification. The productive period ended after three harvests. At this stage, the experiment was conducted under a completely randomized design with a 4 × 2 factorial arrangement with 8 replications. The P. ostreatus strains inoculated in the corn stubble, compared to rice straw, showed 93.93% biological efficiency and a 2.07% production rate, representing increases of 30% and 50%, respectively. The strains PO/A03 and PO/A04 showed higher biological efficiency and organic matter loss. The PO/A02 strain showed greater earliness, with approximately 10 days to harvest. This study concluded that the isolated strains of P. ostreatus allow for the efficient use of corn stubble and rice straw, and can contribute to the management of agronomic wastes. Novelty statement: In developing countries, production of edible mushrooms of the genus Pleurotus can be an efficient tool for alternative and eco-friendly agronomic waste management, allowing for highly nutritious food to be provided to the population through the bioconversion of lignocellulosic compounds. This system has a low production cost and the postharvest substrate, Spent Mushroom Substrate (SMS), can be used as organic fertilizer or animal feed. The aim of this experiment was to contribute to the technical and scientific information on the productive characterization of four Pleurotus ostreatus (Jacq.) strains occurring in the Peruvian region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of lignocellulosic corn and rice wastes as substrates for oyster mushroom (Pleurotus ostreatus Jacq.) cultivation

et al. 2020

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“…Generally, mushroom polysaccharides are prepared from the fruiting bodies, mycelia and/or submerged fermentation broth [ 4 – 6 ]. In most cases, mushroom fruiting bodies grow on the solid substrates including crop by-products such as cottonseed hulls [ 7 ] and corn distiller’s waste [ 8 , 9 ] for 2–4 month cultivation. Mycelia or fermentation broth is a promising alternative to meet the increasing demand on the commercial mushroom products with shorter culture period, consistent product quality and independent of seasonality [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc

et al. 2018

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BackgroundMushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation. Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production. Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.ResultsAddition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia. Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc. Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.ConclusionOur findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.

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“…The suspensions were filtered through a nylon membrane (40 mm; Millipore, Billerica, MA, USA) and resuspended to about 1.4 Â 10 8 conidia per ml, as determined by direct counting using a hemocytometer. Pleurotus The fructification of P. eryngii KNT2312 was analyzed as reported previously [22]. At the end of the mycelial run (35 days at 25 C), the old spawn and top layer of substrate ($1 cm) were removed by scraping to induce fruiting.…”

Section: Pathogenicity Testmentioning

confidence: 99%

“…The control culture was inoculated with distilled water. The fruiting bodies were harvested before the pileus had opened completely to determine various phenotypic traits, including the yield, pileus, and stipe, using calipers (Mitutoyo, Tokyo, Japan) and a balance as described previously [22]. The earliness (days to harvest after removing old medium) was also measured.…”

Section: Pathogenicity Testmentioning

confidence: 99%

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

Lee¹,

Jung²,

Hong

et al. 2020

Mycobiology

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In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1-a (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt).

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Development of cultivation media for extending the shelf-life and improving yield of king oyster mushrooms (Pleurotus eryngii)

Cited by 37 publications

References 31 publications

Use of lignocellulosic corn and rice wastes as substrates for oyster mushroom (Pleurotus ostreatus Jacq.) cultivation

Use of lignocellulosic corn and rice wastes as substrates for oyster mushroom (Pleurotus ostreatus Jacq.) cultivation

Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

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