Use of lignocellulosic corn and rice wastes as substrates for oyster mushroom (Pleurotus ostreatus Jacq.) cultivation

Abstract

The accumulation of agronomic wastes has caused several environmental problems, such as air and soil contamination, and insect and pathogen proliferation, among others. To mitigate this, studies have evaluated the use of these wastes as substrates for the cultivation of Pleurotus mushrooms, a low-cost/nutritionally important crop. This study aimed to evaluate the use of corn stubble and rice straw as substrates for the in vitro production of oyster mushrooms (Pleurotus ostreatus Jacq.) and the productive characterization (biological efficiency, production rate, earliness, daily productive capacity) of four isolated strains. The strains PO/A01, PO/A02, PO/A03, and PO/A04 were grown in Potato-Dextrose-Agar medium until complete colonization. The experiment was evaluated under a completely randomized design with 6 replications. Subsequently, the corn stubble and rice straw were disinfected, inoculated, bagged, incubated, and subjected to induction-fructification. The productive period ended after three harvests. At this stage, the experiment was conducted under a completely randomized design with a 4 × 2 factorial arrangement with 8 replications. The P. ostreatus strains inoculated in the corn stubble, compared to rice straw, showed 93.93% biological efficiency and a 2.07% production rate, representing increases of 30% and 50%, respectively. The strains PO/A03 and PO/A04 showed higher biological efficiency and organic matter loss. The PO/A02 strain showed greater earliness, with approximately 10 days to harvest. This study concluded that the isolated strains of P. ostreatus allow for the efficient use of corn stubble and rice straw, and can contribute to the management of agronomic wastes. Novelty statement: In developing countries, production of edible mushrooms of the genus Pleurotus can be an efficient tool for alternative and eco-friendly agronomic waste management, allowing for highly nutritious food to be provided to the population through the bioconversion of lignocellulosic compounds. This system has a low production cost and the postharvest substrate, Spent Mushroom Substrate (SMS), can be used as organic fertilizer or animal feed. The aim of this experiment was to contribute to the technical and scientific information on the productive characterization of four Pleurotus ostreatus (Jacq.) strains occurring in the Peruvian region.

Olive and green tea leaves extract in Pleurotus ostreatus var. florida culture media: Effect on mycelial linear growth rate, diameter and growth induction index

“…In concurrence, the MLGR was significantly higher in T2 (culture media based on sorghum grains) in comparison with wheat and maize-based cultures (T1 and T3) (higher by 2.6 mm/day and 1.0 mm/day, respectively) (table 9). An earlier study found that the linear growth rate of P. ostreatus on wheat grains was around 3.5-4.0 mm/day at an inoculation temperature of 25°C [26] being lower by 40-46% lower than in the current study. An MGR of 31.5-38.6 mm was acknowledged after 8 days of inoculation on white and red sorghum grains [27] being much lower than in the current study (90 mm).…”

“…This may point the role of tea extract when combined with olive leaves one in the hastening of mycelial growth. Green tea extract contains valuable enzymes [26] that may have been liberated and thus helped in the decomposition of holocellulose compounds (found in olive and tea extracts) and the provision of needed energy for P. ostreatus mycelium growth [9,10].…”

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

“…Many reports have discussed the many purposes of oyster mushrooms through their cultivation on agro-wastes or agro-industrial wastes, which include mushroom production, and the biodegradation of agro-wastes, producing many bioactive compounds and creating a healthy environment for humans, as well as these mushrooms possessing pharmaceutical attributes for medical applications, such as anti-diabetic, anti-carcinogenic, anti-oxidative, and immune suppressor attributes (e.g., [11,12,61,[112][113][114]. Furthermore, oyster mushrooms have a great ability to convert the agro-wastes into bioenergy, bio-compost and biofertilizers [60,115].…”

Potential Enhancement in The Nutritional Value of Local Agro-Waste Through Cultivation of Pink Oyster Mushroom (Pleurotus djamor)

“…Previous research showed an increase in mycelium run when two or more substrates were combined (Baysal et al, 2003;Mane et al, 2007;Owaid et al, 2016). The growth rate of mycelium is affected by substrate chemical composition, plant structure, origin, particle size, and the presence of polyphenols (Jonathan et al, 2012;Zárate-Salazar et al, 2020).…”